Antitumor effect of corosolic acid and it’s impact on CAM and YSM angiogenesis / 中国生化药物杂志

Objective To evaluate the antitumor effect of corosolic acid and its impact on CAM and YSM angiogenesis.Methods The effects of CRA on A549 proliferation was studied by MTT method in vitro.Tumor-bruden nude mice model were established by injecting A549 lung cancer cell marked with bioluminescent to nude mice,and the growth of tumor in mice were detected by IVIS small animal in vivo imaging system.Experiment of chicken embryos eggs are used to observe the role of drugs on CAMand YSMblood vessels. Results The value of IC50 of CRA for A549 in vitro was 26.8μg/mL.A tumor-burdened animal experiment results showed that the CRA to A549 solid tumor has a certain therapeutic effect.CAM and YSM blood vessels of chicken embryos eggs treated with CRA were decreased significantly than negative control group.Conclusion CRA has certain therapeutic effect for A549,which may be related to the inhibition of angiogenesis in tumor tissues.

Spinal bone mineral density related with YSM in Korean menopausal women

BACKGROUND: Estrogen deficiency after menopause is a major reason of bone loss in postmenopausal women. YSM (years since menopause) is negatively related with BMD in postmenopausal women. Bone loss after menopause is accelerated in early stage of menopause, and then decreases. There are some evidence that body weight can prevent bone loss in menopausal women. Therefore, we evaluated associations of the general characteristics and lumbar BMD of postmenopausal Korean women, and determined the rate of bone loss after menopause at 5-year interval. METHODS: BMD was measured by DEXA system and physical, anthropometric data including body fat were obtained in 1006 peri - and postmenopausal women. Pearsons correlation coefficient were made among pesical, anthropometric data and lumbar BMD. To determine the contributable factors for lumbar BMD, multiple regression analysis was done. RESULTS: 1) In Pearsons correlation analysis among body composition YSM and spinal BMD, YSM was strongly associated negatively with spinal BMD. 2) On the other hand, weight and BMI was associated positively with spinal BMD. 3) The percent difference in spinal BMD between perimenopause and 5-years after menopause was 15.3% 4) Years since menopause, body weight was revealed as an important factor that predicted spinal BMD by multiple stepwise regression analysis. CONCLUSIONS: We conclude that YSM and body weight were revealed as important factors that can predict spinal BMD in postmenopausal women.

Bone Mineral Density in Postmenopausal Women / 대한산부인과학회잡지

To examine the influence of the duration of menopause on bone loss, 60 healthy women, aged 34~74 years in 1995 were studied. After exclusion of past or present hormonal replacement therapy or past history of chronic disease, 60 women formed the final study population. Bone masses were measured at 3 sited of femur(femoral neck, Wards triangle and trochanter) and in the L2~L4 lumbar spine by Dual energy X-ray bone densitometry(DEXA). Correlation analysis and multiple linear regression analysis were used to analyze the relationships of gynecological variables(age, body mass index:BMI), and years since menopause(YSM) with bone mineral density(BMD) of lumbar spin and the three different sites of femur. The coefficients of correlation(r) of the bone mineral density(%) at the lumbar spine and the three different sites of femur with YSM were ranged -0.37~-0.48(p < 0.01). Only YSM was independently related to BMD(%) data at all sites, except Wards triangle by multiple linear regression analysis(p < 0.01). In conclusion, the menopause and the duration of menopause have a great effect on bone mineral loss.