ABSTRACT
ABSTRACT The present study was undertaken to verify the hypoglycemic potential of unripe and ripe fruit extracts of Musa sapientum by using various in-vitro techniques, namely glucose adsorption capacity, glucose diffusion, amylolysis kinetics and glucose transport across the yeast cells. The results revealed that the unripe and ripe fruit extracts of Musa sapientum adsorbed glucose and the adsorption of glucose increased remarkably with an increase in glucose concentration. There were no significant (p≤0.05) differences between their adsorption capacities. In the amylolysis kinetic experimental model the rate of glucose diffusion was found to be increased with time from 30 to 180 min and both extracts exhibited significant inhibitory effects on the movement of glucose into external solution across the dialysis membrane as compared to control. The plant extracts also promoted glucose uptake by the yeast cells and enhancement of glucose uptake was dependent on both the sample and glucose concentration. The hypoglycemic effect exhibited by the extracts was observed to be mediated by inhibiting α-amylase, inhibiting glucose diffusion by adsorbing glucose and by increasing glucose transport across the cell membranes as revealed by an in-vitro model of yeast cells.
Subject(s)
Musa sapientum/analysis , Hypoglycemic Agents/adverse effects , Plant Extracts/analysis , Diabetes Mellitus , Fruit/classification , GlucoseABSTRACT
Neste trabalho, avaliou-se a adição de células íntegras de levedura e seus derivados em dietas para juvenis de tilápia do Nilo. Foram utilizados 144 juvenis machos de tilápia (peso médio de 52,1g) distribuídos em 12 tanques de fibra de vidro (250L), em delineamento inteiramente casualizado, composto por quatro tratamentos e três repetições. Os peixes foram alimentados ad libitum, duas vezes ao dia durante 60 dias, com dietas isoproteicas (28 por cento PB) e isocalóricas (2.900kcal de ED kg-1) contendo levedura íntegra de cana-de-açúcar (LI), levedura autolisada (LA) e parede celular (PC) adicionados na proporção de 25 por cento da proteína bruta total, comparadas com uma dieta controle (CO), sem adição de levedura. Não foram observadas diferenças significativas para conversão alimentar aparente e taxa de eficiência protéica. No entanto, o ganho em peso foi melhor nos peixes alimentados com as dietas LA (114,70g) e PC (131,03g), assim como em relação à taxa de crescimento específico (LA=1,79 e PC=1,93 por cento), à proteína bruta no ganho de peso (LA=14,45 e PC=15,62 por cento) e ao conteúdo corporal proteico (LA=14,89 e PC=15,67g 100g-1). As frações, a parede celular e a levedura autolisada de cana-de-açúcar podem ser utilizadas em dietas para juvenis de tilápia.
This study has evaluated the effect of adding dried yeast and its by-products in diets for juveniles of Nile tilapia. It was used 144 juveniles of male tilapia (average weight of 52.1g) distributed in 12 fiberglass tanks (250L), in completely randomized design, composed by 4 treatments and 3 replicates. The fish were fed ad libitum, 2 times per the day during 60 days, with isoproteic (28 percent CP) and isocaloric diets (2.900kcal ED kg-1) with dried yeast of sugar cane (LI), disrupted yeast cells (LA) and yeast cell wall (PC) added in the ratio of 25 percent of the total crude protein, compared with a control diet (CO), without yeast addition. No significant differences were observed for feed conversion and proteic efficiency rate. However, the weight gain was better in the fish fed with diets LA (114.70g) and PC (131.03g), as well as in relation to the specific growth rate (LA=1.79 and PC=1.93 percent), crude protein in the weight gain (LA=14.45 and PC=15.62 percent) and body protein content (LA=14.89 and PC=15.67g 100g-1). The by-products, cellular wall and disrupted yeast cell of sugar can be used in diets for tilapia juveniles.
ABSTRACT
Mechanisms of UV-B-induced apoptotic regulation in yeast Saccharomyces cerevisiae were studied. The results showed that UV-B irradiation indeed inhibited the growth of yeast cells as well as induced extensive apoptosis during 96 h experiment period. However, survival of 96 h irradiated cells remained 10% while most control cells finally dead after re-growth under UV-B irradiation for 12 d. And by exposed to 0.01 mol/L or 0.1 mol/L H2O2 for 30 min, survival rate of 24 h irradiated cells were 3.0-fold or 3.2-fold than control, respectively. By to heat shock for 30 min or 60 min, survival rate of 24 h irradiated cells were 3.5-fold or 9.0-fold than control, respectively.