ABSTRACT
Objective To obserVe the clinical efficacy and safety of yiqing caPsule in the treatment of acne with concomitant constiPation. Methods A randomized controlled method was used. One hundred and forty_fiVe Patients with acne and concomitant constiPation were randomized into treatment grouP (n=75) and control grouP (n=70). Patients in the treatment grouP receiVed yiqing caPsule orally and comPound tretinoin gel toPically, while Patients in the control grouP receiVed toPical comPound tretinoin gel only. Both grouPs were treated for 3 weeks. Results The theraPeutic effect of the treatment grouP was much better than that of control grouP (P<0. 01). No obVious adVerse reactions were obserVed in either grouP. In the treatment grouP,77.4%of Patients were relieVed from constiPation,the ratio being much higher than the control grouP (P<0.01). The chance of acne relief was remarkably higher in Patients whose constiPation were relieVed (P<0. 01). The SPearman correlation index between acne relief and constiPation imProVement was 0. 699 (P<0. 01). Conclusion Combination theraPy of yiqing caPsule and comPound tretinoin gel is effectiVe in the treatment of acne with no significant adVerse reactions. The constiPation imProVement from yiqing caPsule can contribute to the efficacy towards acne.
ABSTRACT
OBJECTIVE: To study the bacteriostatic action of Yiqing capsule against propionibacterium acnes and staphylococcus epidermidis in vitro.METHODS: Propionibacterium acnes and staphylococcus epidermidis were cultured by microplating method and routine plating,and the bacteriostatic action of different concentration of Yiqing capsule and its main components against propionibacterium acnes and staphylococcus epidermidis were determined by measuring their MICs.RESULTS: The MICs of Yiqing capsule and Scutellaria baicalensis extractum against propionibacterium acnes and staphylococcus epidermidis were 0.98 mg?mL-1 and 1 mg?mL-1,respectively,whereas the MICs of Radix et Rhizoma Rhei and Rhizoma Coptidis Scutellaria extractum against propionibacterium acnes and staphylococcus epidermidis were 1.96 mg?mL-1 and 1 mg?mL-1,respectively.CONCLUSION: Yiqing capsule showed satisfactory bacteriostatic action against the major pathogenic bacteria of acne.
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AIM: The multiple effective components in Yiqing Capsule(Radix et Rhizoma Rhei,Rhizoma Coptidis,Radix Scutellariae) were analyzed by RP-HPLC. METHODS: A column of Kromasil C_(18)(250 mm?4.6 mm,5 ?m)was used.The mobile phase was composed of A 0.5% triethylamine(pH3.0,containing 5 mmol/L SDS),B methanol and acetonitrile(50∶50) with gradient elution.The flow rate was 1.0 mL/min with the detection wavelength at 254 nm. RESULTS: In the linear range the correlation coefficients ranged from 0.999 4 to(0.999 9).The average recoveries were 95.2%-102.2% with corresponding RSD of 0.8%-2.0%,respectively. CONCLUSION: The method is rapid,accurate and reliable.It can be used to control the quality of Yiqing Capsule.
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AIM: To establish a HPLC for determination of baicalin and emodin in Yiqing Capsule(Radix Scutellarial, Radix et Rhizoma Rhei). METHODS:A Kromasil C_(18) Column was used. The mobile phase was methanol -0.2% phosphoric acid for gradient elution. Baicalin and emodin were determined by dual wavelength, ?_1=315 nm, ?_2=266 nm. RESULTS:The linear range of baicalin was within 64.40-322.00 ?g?mL~(-1) (r=(0.999 8)). The average recovery was 99.96%,RSD was 1.57%(n=9). The linear range of emodin was within( 0.82)-13.20 ?g?mL~(-1)(r=0.999 9).The average revovery was 98.90%, RSD was 1.25%(n=9). CONCLUSION: This method has good repeatability and flexibility. It can be used for quality control in production of Yiqing Capsule. (Nan
ABSTRACT
AIM: To determine the contents of baicalin, berberine, rhein in Yiqing Capsule(Radix et Rhizoma Rhei, Radix Scutellariae, Rhizoma Coptidis) by HPLC. METHODS: The HPLC system consisted of Kromasil C 18 column (150 mm?4.6 mm, 5 ?m), 0.02 mol?L -1 phosphoric acid (TEA adjusted to pH=6.82)-acetonitrile(40∶11) mixture as mobile phase, detection wavelength at 254 nm, flow rate of 1.0 mL?min -1. RESULTS: The calibration curves of three ingredients were linear within the range of 3.666-73.32 mg?L -1(r=0.9998, baicalin), 0.3112-15.56 mg?L -1(r=0.9998, rhein), 1.221-50.04 mg?L -1(r=0.9999,berberine), respectively. The average recovery of baicalin is 102.3%(RSD=2.5%), berberine for 101.8%(RSD=1.8%), and rhein for 98.3%(RSD=2.9%). CONCLUSIONS: The method is rapid, sensitive and accurate.