ABSTRACT
OBJECTIVE:To determine the contents of Icariin,Psoralen and Isopsoralen in Yishenling granule by HPLC. METHODS: The chromatographic separation was performed on Phenomenex luna C18 column (250 mm?4.6 mm, 5?m) with mobile phase consisted of acetonitrile-0.1% acetic acid solution (gradient elution) at a flow rate of 1.0 mL?min-1.The UV detection wavelength was set at 310 nm, and the column temperature was 20 ℃. RESULTS: Icariin,Psoralen and Isopsoralen in Yishenling granules were well-separated with good linearity within their respective concentration range. And the average recoveries for the three constituents were all within 99%～101% with RSD all less than 1.20%. CONCLUSION: The method is simple, feasible and reproducible, and it can be used for the quality control of Yishenling granule.