ABSTRACT
Objective To explorethe mechanism and down stream signaling pathways of ZNF185 in esophageal squamous cell carcinoma by transcriptome sequencing and bioinformatics analysis. Methods The expression of ZNF185 was knocked down by conducting shRNA transfection of esophageal squamous carcinoma cell lines,and the changes of mRNA expression were detected by transcriptome sequencing technology. The differential genes were screened based on sequencing data,and the differential gene-related functions and signaling pathways were analyzed by GO functional analysis and KEGG enrichment analysis. The interactions of differential proteins were analyzed by STRING,and the related protein-protein interaction(PPI) network was constructed to analyze the keynode proteins. Results There were 3859 differential genes between shZNF185 group and the control group,including 2112 genes with elevated expressionand 1747 genes with decreased expression. RNA splicing cell-matrix junction,local adhesion were significantly enriched in GO analysis. KEGG enrichment analysis indicated that ZNF185 might be related to HIF-1 signaling pathwayand p53 signaling pathway,and key proteins such as BMP2 and OAS2 were identified in PPI network. Conclusion The possible signaling pathways involved in the regulation of esophageal squamous carcinoma by ZNF185 werescreened out by transcriptome sequencing and bioinformatic analysis, which provide a newidea to elucidate the pathogenesis associated with esophageal squamous carcinoma.
ABSTRACT
Objective To explore the role of zinc finger protein (ZNF)185 in the proliferation of human glioma cells. Methods Human glioma tissues and tumor adjacent tissues were obtained from glioma patients diagnosed pathologically in Tangshan Gongren Hospital from January 2011 to December 2013. Total protein was extracted from different tissues. The ZNF185 expression was detected by Western-blot assay. Total RNA was extracted from tumor adjacent tissues. ZNF 185 cod?ing sequence was obtained by RT-PCR and inserted into pEGFPC2 plasmid to construct the ZNF185 expression vector. Li?pofactamine2000 was used to transfect the ZNF185 expression vector to human glioma cell SF767. pEGFPC2 blank vector transfected SF767 cells were used as control. Changes of cell cycle were analyzed by flow cytometry, and cell proliferation was analyzed by MTT assay. Results The expression of ZNF185 decreased significantly in human glioma tissues compared to that of tumor adjacent tissues(P<0.01). The over expression of ZNF185 in SF767 resulted in the increased proportion of cell cycle G0-G1phase, but decreased proportion of S phase(P<0.05). Furthermore, the cell proliferation was significantly inhibited in the ZNF185 over-expressed SF767 cells compared with that of blank vector transfected cells(P<0.05). Con?clusion ZNF185 plays an inhibitory role in cell proliferation of human brain glioma cells.