ABSTRACT
Objective: To investigate the prototype components and metabolites of Zhidong Granule in rats plasma and urine by UPLC-Q-TOF-MS. Methods: The analysis was carried out on a Waters UPLC HSS T3 (2.1 mm × 150 mm, 1.8 μm) column, with the mobile phase of 0.1% formic acid solution (A)-acetonitrile (B) at a flowing rate of 0.3 mL/min, the column temperature was 35 ℃. Electrospray ionization (ESI) source was applied and operated in both positive and negative ion mode. Results: A total of 45 components, including 28 prototype components and 17 metabolites, were identified by comparing their retention time, ion fragmentation information with that of the blank biological samples, herb extract, and reference compounds. Conclusion: This experimental method is simple and reliable, which can provide theoretical basis for elucidating the bioactive components of Zhidong Granule.