ABSTRACT
Objective To set up a new method for fast determining and identifying Xintong Oral Liquid by acousto-optic tunable filter-near infrared(AOTF-NIR)spectroscopy.Methods Identifying method by NIR spectroscopy combined with principal component analysis(PCA),and determining model of qualitative and quantitative analyses based on PLS1 algorithm.Results The XT-C identifying model could be used to identify Xintong Oral Liquid,the determining model of qualitative and quantitative analyses with better accuracy,RMSEP of the models for puerarin was 0.137 1,the determination coefficients was R2=0.984 5.The correlation coefficient of the true value and predication value from validation was r2=0.996 4.The average recovery of the predication set was 101.9%.RSD for precision was 1.98%,RSD for stability was 1.57%.Conclusion The method is a quick and simple assay technique with low cost,which is able to be used in the qualitative and quantitive analyses on Xintong Oral Liquid.
ABSTRACT
AIM:A new method to rapidly non-destructive quality control Compound Danshen Tablet was developed by portable acousto-optic tunable filter (AOTF)-near infrared (NIR) spectroscopy. METHODS:A portable AOTF-NIR spectrometer was used to test 215 different batches of Compound Danshen Tablet collected from fifty-six pharmaceutical factories. The samples were analyzed by the Unscrambler program using principal component analysis(PCA). Quantitative analytical models invoking the partial least squares (PLS) algorithm simultaneously predicted the contents of four major constituents in Compound Danshen Tablet:salvianolic acid B,tanshinoneⅡA,water extract and alcohol extract. RESULTS:The qualitative analysis models accurately identified the differences between Compound Danshen Tablet and other reference substances. The results of external validation showed by the significant t-test that no significant difference between predictive and the chemical value,RSD was equal to 0. 990 9,0. 991 3,0. 964 0 and 1. 73% ,2. 08% ,3. 32% ,1. 32% ,respectively. CONCLUSION:The method of rapid non-destructive analyzing Compound Danshen Tablet is explored.