Effect of triglitazone on the secretion of vascular active factors from endothelial cells stimulated by AngⅡ / 西安交通大学学报(医学版)

Objective To investigate the effect of PPAR ? ligand triglitazone on the secretion of ET-1and NO from endothelial cells stimulated by AngⅡ.Methods The cultured human umbilical vein endothelial cells(HUVECs) were treated with AngⅡand triglitazone.Chemiluminescence analysis was used to measure the concentration of NO,and radioimmunoassay method was used to detect the production of ET-1.Results The HUVECs were treated with 10?mol/L and 50?mol/L triglitazone.The concentration of ET-1 in cell culture supernatants decreased without statistical significance.The concentration of NO increased significantly compared with that of the control(P

Seperation of B-cell active factor from the bursa of fabricius of Peking Duck / 中国免疫学杂志

Bursa of Fabricius is a very important organ for B-eell development. We sot bursa extracts from 2-3 months old Peking Duck. A partially purified PFC-stimulating active factor has been seperated from the extracts by twice molecular sieve chromatography, Sephadex G-15 and Moselect G-10. The M. W. of the active factor is below 750 dal. The results of HPLC analysis of the factor show that its polarity is relatively strong, and it has more positive charges.The works of purifying and looking for a new assay are still going on.