ABSTRACT
Objective: To evaluate the protective effect of chlorogenic acid (CGA) on carbon tetrachloride (CCl4)-induced liver injury of rats. Methods: The anti-oxidative activity of CGA was investigated with several established in vitro systems. The hepatoprotective activity of CGA against CCl4-induced acute liver injury in rats was studied. The levels of alanine aminotranferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin (TB) were measured. The histopathological examination was carried out to supplement the biochemical results. Results: CGA possessed strong anti-oxidative ability in vitro. The CCl4-induced liver toxicity experiment showed that the rats pretreated with CGA (300 or 500 mg/kg) had lower levels of ALT, AST, ALP, and TB than those of the CCl4-treated group. These data were supplemented with histopathological examination of rat liver sections. CGA did not show any mortality at the dose up to 5000 mg/kg. Conclusion: CGA could protect the liver against CCl4-induced oxidative damage in rats, and the possible mechanism of the activity may be due to its free radical-scavenging and anti-oxidative activity. © 2014 Tianjin Press of Chinese Herbal Medicines.
ABSTRACT
El saltapericos es un juego pirotécnico a base de fósforo inorgánico, cuyo uso está prohibido porque causa daño hepático agudo. Se reporta un caso de intoxicación severa en una niña sobreviviente, quien ingirió una dosis potencialmente letal y recibió asistencia médica tardía. El protocolo terapéutico que se siguió en el presente caso clínico, permitió el logro de una evolución satisfactoria; este tratamiento consistió en descontaminación interna con agua oxigenada y aceite mineral, exsanguinotransfusión y fármacos hipoamonemizantes.
Saltapericos is a pyrotechnic firework containing inorganic phosphorus whose use is banned since causes acute liver damage. A case of severe poisoning is reported in a girl, who consumed a potentially lethal dose and received late medical care. The therapeutic protocol followed in the present clinical case led to a positive outcome; this treatment consisted of internal decontamination with hydrogen peroxide and mineral oil, exchange transfusion and hypoammonemic drugs.
Subject(s)
Humans , Female , Infant , Dehydration/diagnosis , Abdominal Pain/diagnosis , Liver Failure, Acute/physiopathology , Phosphorus/poisoning , Vomiting/diagnosis , Child Care , Endosonography/methods , Hepatomegaly/etiologyABSTRACT
Objective To observe the protective effects of a mixture for protecting liver and supplementing stomach (保肝益胃合剂) on rat acute liver damage induced by carbon tetrachloride (CCl_4). Methods The model of rat acute liver damage was established by injection of CCl_4 2 ml/kg into the abdominal cavity.The rat models were treated respectively by the mixture for protecting liver and supplementing stomach 30 g?kg~(-1)?d~(-1),the polyene phosphatide acid radical choline capsule [Yi Shanfu (易善复), 180 mg?kg~(-1)?d~(-1)],the glycyrrhizic acid diaminogen capsule [Gan Lixin (甘利欣),30 mg?kg~(-1)?d~(-1)] infused into the stomach.The activities of serum alanine transaminase (ALT) and aspartate transaminase (AST) were detected.In the mean time,the liver pathological changes were observed,the degree of liver cell necrosis was evaluated,and the rat mortality was noted in various groups of treatment.Results The values of ALT,AST and the score of liver cell necrosis in the group treated with the mixture for protecting liver and supplementing stomach [(1.168?1.066) kU/L,(1.845?2.212) kU/L,(0.56?0.53) score] were significantly lower than those in the model group [(4.982?3.502) kU/L,(7.030?3.616) kU/L, (1.38?0.92) scores],and all the differences being statistically significant (all P