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Objective: To observe the influence of β peptide dimmer modified by PEG on anti-metastasis activity. Methods: Cell culture plates were coated with fibronectin (FN) as extracellular matrix (ECM). The influence of β 2 and β 2-PEG peptides on the adhesion of tumor cells to FN were observed. The effects of β 2 and β 2-PEG peptides on migration and invasion ability of tumor cells in reconstituted basement membrane were measured by using Transwell Boyden and Matrigel method. Results: Compared with negative control, β 2 and β 2-PEG peptide significantly inhibited the adhesion of SMMC-7721 and HCCLM 6 tumor cells to FN in a time- and dose-dependent manner (P < 0.05). The inhibitory effects of β 2-PEG were stronger than β 2 peptides (P < 0.05). The mobility and invasion of HCCLM 6 and SMMC-7721 cells were obviously inhibited by β 2 peptide and β 2-PEG (P < 0.05). For HCCLM 6 cells, β 2 peptide and β 2-PEG inhibited cell migration were 54.6% and 56.3% and suppressed cell invasion were 36. 8% and 46.6%, respectively. For SMMC-7721 cells, the migration inhibitory rate were 43.6% and 45.7% and invasion inhibitory rate was 33.6% and 35.9% by β 2 and β 2-PEG peptide, respectively. The difference were not significant before and after PEGylation. Conclusions: The β 2 and β 2-PEG peptides specifically inhibite the adhesion of tumor cells to FN in a time- and dose-dependent manner. The anti-adhesion effects are enhanced after PEG modification. The β 2 and β 2-PEG peptides obviously suppress migration and invasion of the two kinds of tumor cells. The difference is not significant before and after PEG modification.
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Objective To observe the expression of leukocyte-function-associated antigen-1(LFA-1)immunoreaction in children with febrile seizures(FS),and explore the neuroimmunomodulation mechanisms in the pathogenesis of FS.Methods Adopting flow cytometry(FCM),the levels of LFA-1 contained in blood serum and peripheral blood mononuclear cells(PBMC)of 60 cases [simple FS(SFS)30 cases;complex FS(CFS)30 cases] with febrile convulsion were analyzed,and compared with those in a normal group(30 cases).Out of 60 children with FS group,the LFA-1 mRNA in 20 cases with SFS and 20 cases with CFS was analyzed,and LFA-1 mRNA in19 health children taken out from control group(30 cases)was analyzed.The real-time PCR was used to detect the expression of PBMC LFA-1 mRNA.Results The expression of LFA-1 in the surface of PBMC of the 3 groups,the highest LFA-1 level was in the SFS group(50.89?21.36),the lowest LFA-1 level was in the CFS group(34.35?11.45),and control group was(41.39?16.30).Significant differences were found in 3 groups(Pa
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Aim To investigate the regulatory effects of Iptakalim hydrochloride(Ipt) on excess expression of monocyte chemoattractant protein-1(MCP-1), intercellular cell adhesive molecule type-1(ICAM-1),vascular cell adhesive molecule1-1(VCAM-1) mRNA in bovine aortic endothelial cells cultured with high concentration D-glucose.Methods The endothelial cells were divided into three groups: control,model and Ipt group.The MCP-1,ICAM-1 and VCAM-1 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR).Results Compared with control group,MCP-1,ICAM-1 and VCAM-1 mRNA expres sion of model group all increased after treatments with 25 mmol?L~(-1) D-glucose for 16 h,respectively.Compare with model group,the excess expression of MCP-1,ICAM-1 mRNA was blocked by 1～10 ?mol?L~(-1) Ipt added for 20 h in advance,respectively but no significant effect was found in the expression of VCAM-1 mRNA.Conclusion High concentration of D-glucose induced excess expression of MCP-1,ICAM-1 and VCAM-1 mRNA,respectively.The excess expression of MCP-1,ICAM-1mRNA is inhibited by Ipt.
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Aim To study the effects of resveratrol(Res)on the release of soluble adhesion molecules from human umbilical vein endothelial cells(HUVECs)induced by N-formyl-methionyl-leucyl-phenylal-anine(fMLP)and the adhesion between neutrophils and HUVECs.Methods The effects of Res on neutrophils adhesion to human umbilical endothelial cell(HUVECs)triggered by fMLP were examined.The soluble intercellular cell adhesive molecule-1(ICAM-1),the soluble vascular cell adhesive molecule-1(VCAM-1)and E-selectin release from fMLP(10 ?mol?L-1)stimulated HUVECs were determined by ELISA kits.The neutrophils isolated from human vein blood were loaded with Fluo-3,a fluorescent indicator,to detect intracellular free calcium concentration([Ca2+]i),and CLA was used as a chemiluminescent indicator to determine superoxide production in neutrophils.Results Res(1~50 ?mol?L-1)significantly inhibited neutrophil adhesion to fMLP-stimulated HUVECs and also obviously downregulated the levels of ICAM-1,VCAM-1 and E-selectin in supernatant of HUVECs culture stimulated by fMLP in the dose-dependent pattern.Res also suppressed fMLP-activated superoxide generation and[Ca2+]i increase in neutrophils.Conclusions Res involved in neutrophil adhesion to HUVECs intermediated by cell adhesive molecules expression trigged by[Ca2+]i and superoxide production in neutrophils,which means a lot to prevent inflammatory diseases.