ABSTRACT
Objective:To explore the therapeutic effect of adipose-derived stem cells (ADSC) on long-wave UV damage in mouse skin in order to provide ideas for the treatment of skin photodamage.Methods:The inguinal and perirenal adipose tissues of C57BL/6 mice were extracted and processed to obtain mouse ADSCs, and the surface markers, adipogenic and osteogenic differentiation capabilities were identified. The mouse photoaging model was irradiated with the SS-03AB UV illuminator, the total UVB dose was 9.45 J/cm 2, and the total UVA dose was 94.5 J/cm 2. Experimental mice (72 in total) were divided into normal group, model group, DMEM (medium) group and ADSC group, each with 18 mice. In the normal group and model group, the materials were taken two weeks after the end of irradiation. After irradiation, the ADSC group was given a subcutaneous injection of 200 μl ADSC suspension, and the DMEM group was given 200 μl of serum-free medium for treatment, and the materials were taken for pathological staining after 2 weeks. The experimental data was processed by analysis of variance. This study was carried out from August 2018 to July 2019 in the First Affiliated Hospital of Zhengzhou University. Results:The extracted cells were identified as adipose-derived stem cells. HE staining showed that the inflammatory cell infiltration in the ADSC group was significantly reduced compared with the DMEM group ( t=20.649, P<0.001) and the normal group ( t=16.147, P<0.001), and the thickness of the dermis layer was significantly increased. Masson staining showed collagen fibers were arranged neatly and the density increased significantly after ADSC treatment. Conclusions:Subcutaneous injection of ADSC can reduce inflammation, promote collagen tissue proliferation, increase the thickness of the dermis, effectively resist inflammatory damage and collagen breakdown caused by UVB.
ABSTRACT
[Objective]To investigate the methods of preparing acellular cartilaginous matrix(ACM) and constructing the tissue engineered cartilage with adipose-derived stem cells(ADSCs)-seeded acellular cartilaginous matrix in vitro.[Method]The ADSCs were isolated from adult New Zealand albino rabbits by collagenase,cultured and amplified in vitro.Fresh cartilage isolated from adult New Zealand albino rabbits were freeze-dried for twelve hours and then treated with Triton X-100,Dnase and RNase so as to obtain the ACM.After sterilized with ultra-violet,the ADSCs were seeded in the acellular cartilaginous matrix at a final density of 2?107/L and cultured in chondrogenic differentiation medium for two weeks in order to construct tissue engineered cartilage.Histology,immunohistochemistry and transmission electron microscope(TEM) were applied to examine the fresh ACM and tissue engineered cartilage.[Result]The test of hematoxylin-eosin(HE) staining and TEM showed no cellular structure in the ACM with only recesses left by removed cells.The ACM had suitable interval porosity and aperture size.After integrated with ADSCs,cells migrated into the ACM and adhered to the surface of material and grew well.After cultured in chondrogenic differentiation medium for two weeks,immunohistochemical staining of type Ⅱ collagen showed part of the cells in the material had enhanced expression of type Ⅱ collagen.[Conclusion]ACM can be used as scaffold material in cartilage tissue engineering.If it was seeded with ADSCs and cultured in chondrogenic differentiation medium,ACM can be used to construct cartilage tissue successfully.