ABSTRACT
Objective To observe the dynamic changes of adrenomedulin(ADM) and calcitonin-gene-relat-ed peptide(CGRP) at different time before,during and after laparoscopic operation for treating CO2 pneumoperitone-urn. Methods 47 cases undergoing laparoscopic operation in our hospital were selected. Plasma ADM and CGRP levels were determined by RIA before anesthesia(T0),10 min before (T1) and 10 min (T2) ,30 min (T3),60 min (T4),and 30 min (TS) after CO2 pneumoperitoneum was treated. Results There was statistical significance in ADM and CGRP level at the different time points before and after CO2 pneumoperitoneum (F=9.686 and 49.804, P<0.01). There was no significance in ADM and CGRP level at TO and T1 (P>0.05);ADM level would decrease at T2 (P<0.05) but CGRP level decrease slowly with no remarkable change as compared with that before CO2 pneumoperitoneum(P>0.05). With time going on, ADM and CGRP level at T3 and T4 was remarkably lower than that at T1 (P<0.01);ADM and CGRP level at T5 would come to that at T1. Conclusions CO2 pneumoperitoneum during laparoscopic operation is the major factor causing decreased ADM and CGRP level;The decrease of ADM and CGRP level will influence the cardiac endocrine function,so CO2 pneumoperitoneum operation should be taken seri-ously when the patients have heart disease and cryptorrhea.
ABSTRACT
Adrenomedulin is an angiogenesis peptide that was found recently.It plays a critical role as an autocrine/paracrine tumor cell survival factor.Recently,adrenomedulin has been proved that it is overex- pressed in tumor,such as breast cancer.It may have a significant role in tumor progression and differentiation by stimulating mitogenic activities,inhibiting immune response,inhibiting apoptosis and enhancing angiogen- esis.We may realize targeted therapy tumor by intervening adrenomedulin,its receptor,and the signal con- duction pathway.
ABSTRACT
AIM:To observe the neuroprotection effects exogenous adrenomedullin(AM) against hypoxic-ischemia reperfusion damage(HIRBD) in neonatal rat models.METHODS:Forty neonatal rats were randomly divided into Control Group(n=8,with sham operation),Hypoxic-ischemia Reperfusion(HIR) Group and Intervention Group(n=8,wth intraperitoneal AM injection 0,6 and 12 h after making model).All rats were decapitated at 48 hours after reperfusion,and the cerebral slices were made at the 2 mm level in front of the interaural line.The pathobiological changes of brain tissue were observed by the method of HE dye in light microscope,and the levels of nitrogen monoxide synthase(NOS),glutathion(GSH) and myeloperoxidase(MPO) in neonatal rats cerebral tissue were determined by using chromatometry.RESULTS:Hypoxic-ischemia reperfusion could induce obviously cerebral pathological change which could be improved by AM.The brain tissue levels of cNOS and GSH in AM 0 and 6 h Group were significantly higher than those of HIR Group(P