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Background: Cellular glycosylation changes are associatedwith different types of neoplastic transformation. Fucose is adeoxyhexose sugar that the body requires for optimal functionsof cell to cell communications and which plays a role in severalbiological events. Fucose has been considered to play asignificant role in cancer and its spread. Alpha L Fucosidase(ALF) is an exoglycosidase involved in the hydrolyticdegradation of fucose containing components of glycoproteins,glycolipids and oligosaccharides. The significance of thisenzyme in human catabolism is implied by geneticneurovisceral storage disease. Altered levels of ALF has beenreported in the plasma/serum of patients with oral cancer.Aims: To investigate the clinical usefulness of serum fucoseand α-L-fucosidase in diagnosing oral pre-cancer and cancerand study the variations of the levels of both metabolites innormal, precancerous and cancerous conditions (Squamouscell carcinoma).Methodology: The study group comprised of 87 samples of(age range: 20-70 years): control samples – healthy individualswithout any systemic illness (n =20), clinically andhistopathologically diagnosed cases of leukoplakia (n=16) andoral submucous fibrosis (n=16) and oral squamous cellcarcinoma (n=35) respectively. 2ml blood was collected byvenipuncture from every subject after informed consent, serumwas separated and checked for fucose and fucosidase byspectrophotometric analysis.Results: The Normal value range of fucose is 8.3 to 9.5 mg/ dland that of fucosidase is 22.8 ± 7.1 U/L. There is an increasein the value range of fucose and fucosidase in the tissues ofpotentially malignant disorders and Squamous cell Carcinoma.
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Objective To explore the four tumor markers of alpha-fetoprotein ( AFP ), α-L-fucosidase( AFU), carbohydrate antigen 199 ( CA199) and carcinoembryonic antigen ( CEA) and their combined use for primary hepatocellular carcinoma (HCC) diagnosis and treatment value. Methods From February 2016 to August 2018,92 patients with primary hepatocellular carcinoma (HCC group),79 patients with benign liver disease (chronic hepatitis and cirrhosis group) and 99 healthy adults (control group) were selected as subjects. The serum levels of four tumor markers in different populations were compared. Results The serum levels of four tumor markers ( AFP ( 192. 4 ± 89. 3) μg／L、AFU( 78. 6 ± 25. 8) U／L、CA199 (107. 2 ± 59. 5) U／mL、 CEA ( 37. 9 ± 14. 9) μg／L) were significantly higher than those of benign liver disease group(AFP( 17. 4 ± 6. 3) μg／L、AFU( 35. 4 ± 17. 2) U／L、CA199( 29. 3± 15. 2) U／mL、CEA( 4. 9 ±1. 7) μg／L) and normal people( AFP(4. 8±1. 1) μg／L、AFU(12. 2±3. 6) U／L、CA199( 6. 4± 2. 3) U／mL、CEA(1. 8±0. 4) μg／L) . There differences had significant ( all P＜0. 05) . The abnormal rate of single factor in hepatocellular carcinoma group ( AFP 84. 8%, AFU 52. 2%, CA199 41. 3%, CEA35. 9%) was significantly higher than that in benign liver disease group ( AFP 15. 2%, AFU 19. 0%, CA19916. 5%, CEA13. 9%) and normal group (AFP 4. 0%,AFU 5. 0%,CA199 3. 0%,CEA 6. 0% ug／L),the difference was statistically significant ( all P＜0. 05) . The highest sensitivity was AFP ( 84. 8%) and the highest specificity was AFP and CA199 (91. 0%). The sensitivity of combined detection was 94. 6% higher than that of single index ( AFP 84. 8%, AFU52. 2%, CA199 41. 3%, CEA35. 9%) . Conclusion The combined detection of AFP,AFU,CA199 and CEA can increase the sensitivity of diagnosis of hepatocellular carcinoma and reduce the rate of missed diagnosis, which will be beneficial to the early diagnosis and treatment of hepatocellular carcinoma.
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Objective To study the detection value of serum alpha -L -fucosidase(AFU)and alpha fetoprotein 3(AFP -L3)in primary hepatocellular carcinoma(HCC).Methods 100 patients with primary HCC were enrolled as HCC group,54 cases without HCC were selected as non HCC group,100 healthy persons were selected as healthy group.Serum AFU and AFP-L3 levels in three groups were determined.The serum AFU was measured by the chemical colorimetry,and the AFP-L3 level was determined by chemiluminescence immunoassay.Results The serum levels of AFU in HCC group,non HCC group and healthy group were (71.61 ±3.01)U/L,(11.06 ±2.15)U/L, (8.54 ±1.28)U/L.The serum levels of AFP-L3 in HCC group,non HCC group and healthy group were (30.14 ± 2.93)%,(3.27 ±0.83)%,(2.77 ±0.62)%.The serum levels of AFU and AFP-L3 in HCC group were signifi-cantly higher than those in non HCC group and healthy group (t=13.172,9.770,16.036,7.160,all P0.05).The positive rates of AFU were 87.00%,9.26%and 3.00%in HCC group, non HCC group and healthy group.The positive rate of AFU in HCC group was significantly higher than that in non HCC group and healthy group (χ2 =34.06,57.62,all P0.05).The positive rates of AFP-L3 were 89.00%,7.41%and 2.00%in HCC group,non HCC group and healthy group.The positive rate of AFP-L3 in HCC group was significantly higher than that in non HCC group and healthy group (χ2 =41.06,77.62,all P0.05 ).There were no significant differences in serum tumor diameter about AFU, AFP-L3(t=0.167,0.579,0.201,0.601,all P>0.05),and there were significant differences about TNM stage, lymph node metastasis,and tumor thrombus (t =6.156,9.105,10.161,8.120,9.770,12.821,all P<0.05). Conclusion Serum AFU and AFP -L3 can not only help the diagnosis of primary liver cancer,but also can determine the prognosis of patients.
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Objective To investigate the change of serum alpha-L-fucosidase(AFU)and its correlation with the blood glucose and lipid level in small for gestational age(SGA)fetuses. Methods 125 SGA fetuses and 128 fetuses in appropriate for gestational age(AGA)with wet lung were treated in our hospital and were investigated as case control study. The serum of AFU ,blood glucose and lipid were measured and compared within 24 hours after birth in these 2 groups. Results Comparing with the AGA infants ,the SGA babies had lower level of serum AFU,high density lipoprotein,apolipoprotein A and apolipoprotein B(P<0.05). The correlation analysis showed that the serum AFU level has positive correlation with blood glucose,total cholesterol,high density lipoprotein, low density lipoproteinand apolipoprotein A(P < 0.05),while it has negative correlation with serum triglycerides in SGA(P<0.05). Conclusions The SGA infants have lower level of serum AFU and lipid metabolic disorders after birth,and its serum AFU level has correlation with its blood glucose and lipid level.
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Objective Explore the clinical application values of Golgi Protein 73 ( GP73 ) , AFP variants (AFP-L3) , Alpha fetoprotein ( AFP) and α-l-Fucosidase ( AFU) detection in the diagnosis of hepatocellular carcinoma ( HCC) .Methods From January of 2013 to June of 2014, 84 case of HCC Patients( HCC group ) who presented at interventional department; 64 case of cirrhotic patients ( liver cirrhosis group ) , 86 case of chronic hepatitis patients ( chronic hepatitis group ) and 120 healthy people ( normal control group) were selected from Shanghai Tongren Hospital.GP73 was detected by the enzyme-linked immunosorbent assay (ELISA), AFP-L3 was isolated with ACSC, AFP and AFP-L3 were detected with ECLIA and calculated the percentage content of AFP-L3 ( AFP-L3%) , AFU was detected with enzyme kinetic method.Adopted the SPSS 19.0 statistical software for data analysis.The rank sum test was used in the multi group comparison;the chi square test was used in the comparison group.Results Serum levels of GP73, AFP-L3, AFP and AFU in HCC group were 202.1 μg/L, 9.5%, 68.3 μg/L, 33.2 μg/L respectively.Their difference from those of the normal control group(69.0 μg/L,2.5%,4.5 μg/L,24.2μg/L) was of statistical significance (U was 1126.59, 204.67,1247.68,564.08,respectively,all P 0.05) Sensitivity of GP73 and AFP in individual inspection was 95.24%, significantly higher than that of AFU, AFP-L3. Specificity of AFP-L3 was 94.81%respectively, with an accuracy of 85.88% respectively.Specificity and accuracy of the allied detection of GP73, AFP-L3, AFP and AFU for HCC diagnosis were 98.52% and 84.75% respectively.Conclusions The allied combination of serum GP73, AFP-L3, AFP and AFU makes up for the insufficient clinical applications of individual serum markers. It is of great clinical significance to improve the diagnosis of HCC.
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Objective To investigate the application of maternal serum α-L-fucosidase to the placental function monitoring. Methods Comparative analysis of the changes of serum α-L-fucosidase between 120 patients with advanced placental dysfunction, pregnant women with different stages of gestation and 80 healthy non-pregnant woman was performed,and the correlation of the se-rum α-L-fucosidase of 120 cases with advanced placental dysfunction with UE3 and HPL was analyzed.Results The level of serumα-L-fucosidase of patients with placental dysfunction was lower than that of normal pregnant women,and the difference was statisti-cally significant (P <0.05 ).The serum α-L-fucosidase was correlated with UE3 and HPL which were the indicators of placental function monitoring (r=0.534 and 0.587,P <0.05).Conclusion Serum α-L-fucosidase can serve as the conventional biochemical indicator for monitoring placental function.
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Objective To explore the correlation between the joint detection of cholinesterase(CHE),alpha-L-fucosidase(AFU) and alpha-fetoprotein(AFP)and primary hepatic cancer(PHC).Methods 56 cases of patients with PHC(PHC group),52 cases of patients with liver cirrhosis(LC group)and 60 cases of healthy individuals(control group)were enrolled in this study,and serum levels of CHE,AFU and AFP were detected and statistically analysed.Results Serum levels of CHE in patients with PHC were negatively correlated with levels of AFU and AFP(correlation coefficient was -0.889 and -0.797 respectively,P <0.05),the ser-um levels of CHE in patients with LC were also negatively correlated with levels of AFU and AFP (correlation coefficient was-0.598 and -0.653 respectively,P <0.05).The positive rates of joint detection of CHE,AFU and AFP in PHC group and LC group were higher than that in the control group,had statistically significant differences(P <0.05 ).Conclusion CHE,AFU and AFP are sensitive indicators of liver lesions,and the joint detection could provide reference value for diagnosis and monitoring pro-gression of PHC.
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Objective:To approach the prediction value of alpha-fetoprotein(AFP) and ?-L-fucosidase(AFU) during the hepatoma recurrence post of liver transplantation.Methods:Thirty-five patients with hepatoma recurrence underwent transplantation of liver were followed up and observed.The changes of AFP and AFU in blood serum during the hepatoma recurrence post-transplantation of liver were analyzed.Results:It was followed up 2 to 12 months,10 patients were lost.The average time was(5.75?2.46) months.The rest 25 patients were all suffered hepatoma recurrence.There were 12 in liver recurrence(48%) ,13 recurrence outside(lung 36%,bone 4%,portal vein cancer blot 4%,crinosity transfusion 8%) .Conclusion:The sensitivity and specificity of AFU is better than AFP in the hepatoma recurrence,AFU is a better blood serum index in following up the hepatoma recurrence post liver transplantation.
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Objective To study the diagnostic value of AFP,AFU and the combined detection of AFU and AFP in PHC.Methods To analyse the detection results of AFP,AFU and AFP and AFU from 80 PHC patients and 40 healthy controls by ROC curve.Results AFP and AFU were significantly different between them (P