ABSTRACT
Objective The current study was aimed to establish an electrically amygdala kindling model of refractory epilepsy in Sprague-Dawley rats, and to study their changes of electrocorticagram ( ECoG ) .Materials and methods Male Sprague-Dawley rats were used in the experiment.Two-polar electrode was implanted into the right amygdala stereotactically.Two weeks after the surgery, electrical stimulations were given to elicit the grade 4 seizure in all animals with the fast kindling protocol.Rekindling was administrated after the first kindling.The after discharge threshold ( ADT) and the number of stimulus were reassessed in the two kindling protocols.ECoG and the behavior of the animals were recorded during the whole experiment.Result The changes of ECoG and behavior: Animals showed stage 1 -3 seizure when the ADT was assessed.While during the kindling period, the animals showed generalized convulsion with stage 4-5 seizure.ECoG showed sharp waves, spike waves, sharp-slow waves and spike-slow waves.Statistical analysis showed that the ADT was not significantly increased at two weeks after kindling ( from 83.33 ±22.29μA to 84.17 ±16.76μA, P=0.923 ) .The number of stimulus given to elicit the stage 4 convulsion was not significantly increased as well ( from 4.41 ±2.27 to 5.58 ±3.96, P=0.231 ) .Conclusions Rapid kindling model of epilepsy in rats is an effective epilepsy model, which is stable for 2 weeks.
ABSTRACT
The purpose of this study was to evaluate the effect of adenosine A2A receptor antagonist ZM241385 on amygdala-kindled seizures and its roles in epileptogenesis.Electrodes were implanted into the right amygdala of male adult Wistar rats.Kindling was accomplished by using stimulus strength of 500 μA applied daily to the amygdala until 10 consecutive stage 5 seizues were induced.Then effect of ZM241385 was studied in fully kindled rats after intracerebroventricular administration of the drug.In addition,the effect on kindling progression was evaluated through ZM241385 injection before daily stimulation.In all experiments,behavioral changes in the rats in response to ZM241385 were monitored closely.The results showed that,in fully amygdala-kindled rats,ZM241385 (0.001-.1 nmol/L) decreased afterdischage duration (ADD),motor seizure duration (MSD),stage 5 duration (S5D) and seizure duration (SD),but only the effect on ADD was dose-dependent.The doses of 0.001-0.1 nmol/L had no influence on stage 4 latency (S4L) and seizure stage (SS).The dosages of 0.0001 and 1 nmol/L of ZM241385 did not exert any effect on all seizure parameters.In contrast to the results in fully amygdala-kindled rats,ZM241385 (0.001-0.1 nmol/L) had minimal or no effects on the progression of amygdala-kindled seizures.We are led to the conclusion that although ZM241385 had no influence on the progression of amygdala-kindled seizures,it had potent anti-convulsant profile and little adverse effects at the dosage of 0.001-0.1 nmol/L,suggesting that the agent is effective against the amygdala-kindled seizures.
ABSTRACT
Objective To investigate the effect of low frequency electric deep brain stimulation on amygdale kindling in rats.Methods The amygdale kinkling model of rats was established by operation on the brain.The effects of low frequency deep brain electric stimulation used alone or in combination with anti-epilepsy drugs were ob- served in terms of severity of seizure attack reflected by Racine's scale and afterdischarge duration recorded in electro- encephalogram.Results Fifteen minutes of low frequency electric stimulation at 1 Hz and 100 to 350?A effective- ly inhibited amygdale kindling as demonstrated by a significant decrease of afterdischarge duration,and decreased the severity of seizure attack (P
ABSTRACT
AIM To investigate the role and mechanism of a novel antiepileptic drug topiramate on amygdala kindling in rats. METHODS The effects and mechanism of topiramate on kindling were examined by the establishment of amygdala kindling model and combination with other drugs. The influence of topiramate on seizures induced by semicarbazide hydrochloride(SCZ) was also observed. RESULTS Topiramate (50～200 mg?kg -1 ,ig) dose-dependently inhibited the seizure severity in amygdala kindling ( P