ABSTRACT
By studying on extraction technology of desertliving cistanche polysaccharides, it provided evidences for development and utilization of cistanche. Anthrone-sulfuric acid method was used in the measurement of desertliving cistanche polysaccharides. The yield of desertliving cistanche polysaccharides was used as index. Different types of extraction methods were compared. Then, the extraction process was optimized by orthogonal experiment. The results showed that the best extraction process was Tween-60 ultrasonic cooperated. The best extraction process was when the mass concentration of Tween-60 was 0.5%, solid-to-liquid ratio was 1:25, temperature was 60℃, and the extraction time was 30 min. The extraction yield of desertliving cistanche polysaccharides was 8.17%. It was concluded that this extraction technology was reasonable and reliable, which can be used in the development and utilization of cistanche.
ABSTRACT
OBJECTIVE:To compare the differences of two methods in the determination of Ganoderma or Ganoderma extract and the related products.METHODS:The content of Ganoderma in samples was determined by phenol-sulfuric acid method vs.anthrone-sulfuric acid colorimetric method,and the differences of the two methods were compared.RESULTS:The two methods showed similar determination results.The linear range of the standard Polysaccharide solution was 0.005～0.1 mg?mL-1(rphenol=0.999 4,ranthrone=0.999 7).CONCLUSION:Both of the two methods can be used to determinate the content of Ganoderma.
ABSTRACT
Objective:To optimize the condition of anthrone-sulfuric acid method to determine the polysaccharide isolated from Bletilla striata by central composite design/response surface methodology.Methods:Dextron T20 was a standard substance, independent variables were the ratio of the polysaccharide and anthrone-sulfuric acid,the time of color hydrolysis and the cooling time at room temperature,dependent variable was the UV absorption,nonlinear mathematic models were used to estimate the relationship between independent and dependent variables.Response surface methodology was used to optimize be condition of determination.Results:The optimum conditions of determination:the volume ratio of the polysaccharide and anthrone-sulfuric acid is 1:2;the time of color hydrolysis is 8.00 min;the cooling time at room temperature is 15.00min.On this condition, there was favorable correlation between the absorption of Dextron T20 and polysaccharide of Bletilla striata.Conclusions: Central composite design/response surface methodology can be used to evaluate the feasibility and applicable conditions of the anthrone-sulfuric acid method to determine the polysaccharide isolated from Bletilla striata using Dextron T20 as a standard substance.