ABSTRACT
Objective To study the role of interleukin-22 (IL-22) in murine asthmatic airway inflammation and airway models, and explore the mechanism of astragaioside (AS) Ⅳin the treatment of asthma.Methods Ovalbumin(OVA) was used as an allergen to sensitize and challenge the mice .Thirty-two female specific-free ( SPF) four-week BALB/c mice were randomly divided into 4 groups:control group , asthma group , budesonide treatment group ( BUD group ) and AS-Ⅳgroup.HE staining and AB-PAS were used to measure the inflammation scores and goblet cells hyperplasia , enzyme-linked immunosorbent assay(ELISA) was used to analyze IL-22 levels in bronchoalveolar lavage fluid (BALF), flow cytometry was performed to analyze the proportion of Th22 cells in spleen single cell suspension , and realtime-PCR was performed to analyze the IL-22 mRNA levels in lung tissue .Results The inflammation scores of asthma group were elevated compared with the control group(P<0.05).An overall reduction of asthmatic airway inflammation was observed in the BUD group and AS-Ⅳgroup by the end of the trial .IL-22 levels in BALF and the proportion of Th22 cells in spleen single cell suspension were significantly increased after treatment in BUD and AS-Ⅳ groups(P<0.01), while the mRNA levels of IL-22 were obviously decreased(P<0.01).Conclusion The increase of IL-22 can induce airway inflammation of asthma . AS-Ⅳcan reduce Th22 cell differentiation and the expression of IL-22, thereby inhibiting the development of airway inflammation of asthma.
ABSTRACT
Objective To study the role of interleukin-22 (IL-22) in murine asthmatic airway inflammation and airway models, and explore the mechanism of astragaioside (AS) Ⅳin the treatment of asthma.Methods Ovalbumin(OVA) was used as an allergen to sensitize and challenge the mice .Thirty-two female specific-free ( SPF) four-week BALB/c mice were randomly divided into 4 groups:control group , asthma group , budesonide treatment group ( BUD group ) and AS-Ⅳgroup.HE staining and AB-PAS were used to measure the inflammation scores and goblet cells hyperplasia , enzyme-linked immunosorbent assay(ELISA) was used to analyze IL-22 levels in bronchoalveolar lavage fluid (BALF), flow cytometry was performed to analyze the proportion of Th22 cells in spleen single cell suspension , and realtime-PCR was performed to analyze the IL-22 mRNA levels in lung tissue .Results The inflammation scores of asthma group were elevated compared with the control group(P<0.05).An overall reduction of asthmatic airway inflammation was observed in the BUD group and AS-Ⅳgroup by the end of the trial .IL-22 levels in BALF and the proportion of Th22 cells in spleen single cell suspension were significantly increased after treatment in BUD and AS-Ⅳ groups(P<0.01), while the mRNA levels of IL-22 were obviously decreased(P<0.01).Conclusion The increase of IL-22 can induce airway inflammation of asthma . AS-Ⅳcan reduce Th22 cell differentiation and the expression of IL-22, thereby inhibiting the development of airway inflammation of asthma.
ABSTRACT
AIM: To examine the quality of Radix Astragali Injection from different manufacturers. METHODS: The HPLC-ELSD method for the determination of astragaloside Ⅳ in Radix Astragali Injection was established with hypersil ODS 2 C 18 column(250mm?4.6mm,5?m), column temperature at 40℃. The mobile phase was acetonitrile-water (36∶64) and flow rate was at 1.0mL?min -1. The evaporation light-scattering detector was used with its drift tube temperature at 100℃ and the gas flow rate: 3.0L?min -1. RESULTS: Within 2.4～12?g astragaioside Ⅳ had a good linearity. The average recovery was 101.6%, RSD=2.9%,n=5. By analyzing the samples of 10 batch of Radix Astragali Injection from 5 manufactuers, it showed there were significant difference in the contents of astragaloside Ⅳ. CONCLUSION: The method is simple and fast. The results are accurate and reproducible and can be used in the quality control of Radix Astragali Injection. The significant differences in the astragaloside content may influence clinical treatmem.