ABSTRACT
Objective To investigate the correlation between atlE gene and biofilm formation of Staphylococcus epidermidis . Methods 64 strains of clinically isolated Staphylococcus epidermidis in our hospital from June 2015 to June 2016 were collected . The biofilm formation test was used to detect bacterial biofilm .PCR was use to amplify atlE gene .Then the correlation between the atlE gene with biofilm formation was analyzed .Results 24 strains of biofilm positive bacterium were detected ,the detection rate was 37 .5% ;31 strains of atlE gene was detected ,the detection rate was 48 .4% ;atlE gene was significantly correlated to biofilm formation(P＜0 .05) .Conclusion Staphylococcus epidermidis has the ability to form biofilm ;atlE gene has a relation with biofilm formation of Staphylococcus epidermidis .
ABSTRACT
O biofilme tem sido considerado o principal fator de virulência dos Staphylococcus epidermidis. A presença do operon ica e de outros genes incluindo atlE e aap, tem sido sugerida como importante para a sua formação. Nosso trabalho teve como objetivo estudar a expressão de biofilme, detectar a presença dos genes icaAD, atlE e aap e determinar a susceptibilidade a antimicrobianos em cepas de S epidermidis resistentes à meticilina (MRSE) obtidas de pacietnes hospitalizados no Hospital Antonio Pedro (HUAP...Nossos dados demonstraram ainda uma correlação entre a característica de forte produçao de biofilme e a presença concomitante dos genes icaAD, atlE e aap. Finalmente, cepas de MRSE multirresistentes estavam mais associadas ao padrão aderente, sugerindo que o ambiente do biofilme possa favorecer as transferências de genes de resistência aos antimicrobianos.