Demineralized dentin matrix technique - a comparison of different demineralizing solutions

Abstract This study aimed to evaluate the microstructure formed after the chemical treatment of teeth, for the development of autogenous grafts from the demineralized dentin matrix (DDM) technique, in order to identify the most efficient demineralizing solution. The specimens, originating from the root and coronal portion, were submitted to ultrasonic cleaning and drying in an oven for 1h at 100 ºC. Then, the density was determined by Archimedes' principle for each specimen, using distilled water as immersion liquid. The samples were separated into five groups: Control group: negative control, Distilled water;EDTA group: positive control, trisodium EDTA; NaOCl group: 2.5% sodium hypochlorite; HCl-0.6M group: 0.6M hydrochloric acid; and H2O2/H2SO4 group: hydrogen peroxide and sulfuric acid. Each specimen was immersed for 1h in the corresponding group descaling solution at 60 ºC. Subsequently, the mass loss and density of the treated specimens were determined by Archimedes' principle. Ultimately, the specimens of each group were characterized by microtomography, Scanning Electron Microscopy, and Energy Dispersive Spectrometry X-ray (SEM-EDS). The results demonstrated that the H2O2/H2SO4 solution allowed the formation of interconnected micropores, suggesting better pore structures for application in scaffolds, when compared to the other studied solutions.

Resumo Este estudo teve como objetivo avaliar a microestrutura formada após o tratamento químico em dentes, para o desenvolvimento de enxertos autógenos a partir da técnica de matriz de dentina desmineralizada (DDM), a fim de identificar a solução desmineralizante mais eficiente. Os espécimes, provenientes da raiz e porção coronal, foram submetidos à limpeza ultrassônica e secagem em estufa por 1h a 100 ºC. Em seguida, a densidade foi determinada pelo princípio de Arquimedes para cada espécime, utilizando água destilada como líquido de imersão. As amostras foram separadas em cinco grupos: Controle: controle negativo, Água destilada; EDTA: controle positivo, EDTA trissódico; NaOCl: hipoclorito de sódio 2,5%; HCl-0.6M: ácido clorídrico 0,6M; e H2O2/H2SO4: peróxido de hidrogênio e ácido sulfúrico. Cada espécime foi imerso por 1h na solução descalcificante de grupo correspondente a 60 ºC. Posteriormente, a perda de massa e a densidade dos espécimes tratados foram determinadas pelo princípio de Arquimedes. Por fim, os espécimes de cada grupo foram caracterizados por microtomografia, microscopia eletrônica de varredura e espectrometria de energia dispersiva de raios-X (SEM-EDS). Os resultados demonstraram que a solução H2O2/H2SO4 permitiu a formação de microporos interligados, sugerindo melhores estruturas de poros para aplicação em scaffolds, quando comparada às demais soluções estudadas.

Therapeutic effect of sural neurocutaneous flap combined with antibiotic-impregnated calcium sulfate and autologous iliac bone graft of chronic calcaneal osteomyelitis / 国际外科学杂志

Objective:To analyze the therapeutic effect of sural neurocutaneous flap combined with antibiotic-impregnated calcium sulfate and autogenous iliac bone graft of chronic calcaneal osteomyelitis.Methods:A retrospective analysis was peformed in 29 patients with chronic calcaneal osteomyelitis treated with sural neurocutaneous flap combined with antibiotic-impregnated calcium sulfate and autogenous iliac bone graft in the Xi′an Honghui Hospital Affiliated to Xi′an Jiaotong University from April 2013 to January 2020. There were 19 males and 10 females, with the age of (45.38±12.85) years, ranged from 22 to 67 years. The course of disease was (16.00±6.96) months, ranged from 6 to 36 months. The skin defect area was (41.9±15.9) cm 2, ranged from 11.8 to 86.8 cm 2. The causes of injury: 18 cases of high fall, 6 cases of traffic accidents, 3 cases of heavy rolling, the remaining 2 cases were machine strangulation and sharp stab wounds. The inflammatory markers [white blood cell (WBC), erythrocyte sedimentation rate (ESR), procalcitonin (PCT), C reactive protein (CRP)] and bone healing time were recorded before operation, 2, 4, 8 weeks and 6 months after operation. During the follow-up period, the flap texture, survival were observed, and the ankle-posterior foot function recovery was evaluated by the American Association of Foot and Ankle Surgery (AOFAS) score were observed before and after the operation, and the incidence of complications were recorded. The measurement data were expressed as mean±standard deviation ( ± s), and the t-test was used for inter-group comparison; the levels of WBC, ESR, PCT and CRP at different time points before and after operation were compared by repeated measurement ANOVA, and the LSD t-test was used for pairwise comparison. Results:All the 29 patients were followed up for (14.51±6.10) months, ranged from 6 to 30 months. All the flaps survived without abrasion, ulceration, or skin protrusion, and all patients could walk normally with shoes. There were 28 cases of stage I bone healing, with an average of (5.87±2.07) months, ranged from 3 to 12 months. The inflammatory indexes was significantly decreased at different time points after operation ( P<0.05). There was no significant difference between 6 months and 8 weeks after operation ( P>0.05), while there was significant difference at other time points ( P<0.05). The ankle-posterior foot score of AOFAS at 6 months after treatment was significantly higher than that before treatment (83.44±7.93 vs 55.37±8.07), the differences was statistically significant ( P<0.05). The clinical efficacy of foot function recovery was excellent in 12 cases, good in 15 cases and fair in 2 cases among 29 patients .The excellent and good rate was 93.1% (27/29). One patient recurred 1 month after operation and was re-implanted with antibiotic-loaded calcium sulfate mixed autogenous iliac bone after debridement, no recurrence was found. The total complication rate was 31.0%, but there was no significant impact on the patient's life in the later period. All patients returned to daily life and work. Conclusion:The treatment of chronic calcaneal osteomyelitis with sural neurocutaneous flap combined with antibiotic-impregnated calcium sulfate and autogenous iliac bone graft can effectively control infection, reconstruct calcaneal and soft tissue structure, promote functional recovery of affected limb, and ultimately improve the patient′s quality of life.

Evaluation of clinical effect of 3D printing-aided autologous tooth transplantation for repairing tooth loss / 西安交通大学学报(医学版)

【Objective】 To evaluate the clinical effect of 3D printing technology in autogenous tooth transplantation for tooth loss. 【Methods】 From September 2017 to August 2021, 169 patients (176 teeth) were selected and analyzed regarding age, gender, recipient site, positioning of the donor tooth, transplantation time, and long-term follow-up. 【Results】 A total of 176 autogenous tooth transplants were completed, consisting of 133 cases of traditional autogenous tooth transplantation and 43 cases of autogenous tooth transplantation with 3D printing technology. The donor tooth separation time for autogenous tooth transplantation with 3D printing technology was significantly less than that for traditional autogenous tooth transplantation (P<0.000 1). A total of nine teeth were removed 3 months after the operation due to loosening(autogenous tooth transplantation failure), among which seven failed in traditional tooth transplantation and two failed in autologous tooth transplantation with 3D printing technology. The success rate of traditional tooth transplantation was 77% and the retention rate was 94.7%. The success rate of tooth transplantation with 3D printing technology was 88%, and the retention rate was 95.3%. 【Conclusion】 3D printed tooth donor model can greatly shorten the time of tooth donor, reduce the damage to the periodontal membrane cells of the transplanted teeth, and improve the success rate of tooth transplantation. It is worthy of broad promotion.

Analysis of the clinical effect of percutaneous pedicle screw fixation combined with transpedicular bone grafting in the treatment of thoracolumbar fracture / 中国骨伤

OBJECTIVE@#To investigate the clinical efficacy of percutaneous screw fixation combined with minimally invasive transpedicular bone grafting and non-bone grafting in the treatment of thoracolumbar fractures.@*METHODS@#From Janury 2021 to June 2022, 40 patients with thoracolumbar fracture were divided into the experimental group and the control group. There were 26 patients in the experimental group, including 21 males and 5 females with an aberage age of (47.3±12.3) years old, who underwent percutaneous pedicle screw fixation combined with transpedicular autogenous bone grafting. In the control group, 14 patients received percutaneous pedicle screw fixation only. including 7 makes and 7 females with an average age of (50.2±11.2) years old. The operative time, intraoperative blood loss, anterior height ratio of injured vertebrae, Cobb angle, visual analogue score (VAS), MacNab scores, loosening or broken of the implants. were compared and analyzed.@*RESULTS@#There was no significant difference in operation time, intraoperative blood loss, VAS and anterior height ratio of injured vertebrae between the two groups. Compared with the preoperative results, VAS and anterior height ratio of injured vertebrae were improved statistically(P<0.05). For Cobb angle of injured vertebra, there was no significant difference between the two groups before surgery (P=0.766). While at 1 week, 3 months and 12 months after surgery, there were statistically differences between the two groups (P values were 0.042, 0.007 and 0.039, respectively). The Cobb angle of injured vertebrae one year after operation was statistically decreased in both groups compared with that before surgery (P<0.001). One year after surgery, the excellent and good rate of Macnab scores was 96.15% in the experimental group and 92.86% in the control group, and there was no statistical differences between the two groups (P=0.648). There was one patient in the control group suffering superficial wound infection on the third day, which was cured by dressing change and anti-infection treatment. There were no postoperative screw loosening and broken in both groups.@*CONCLUSION@#The two surgical methods have the advantages of less trauma, less pain and quicker recovery, which can restore the height of the injured vertebra, reconstruct the spinal sequence and reduce the fracture of the vertebral body. Transpedicular autogenous bone grafting can increase the stability of the fractured vertebra and maintain the height of the vertebra better after surgery, thus reducing the possibility of complications such as kyphosis, screw loosening and broken.

Correlation study on the influencing factors of semitendinosus insertion location / 中国修复重建外科杂志

OBJECTIVE@#To investigate the relationship between the vertical distance from semitendinosus insertion to tibial plateau (S-T) and the physical characteristics of patients, in order to provide reference for incision design to expose the semitendinosus insertion.@*METHODS@#The patients with ligament injury who underwent primary anterior cruciate ligament reconstruction between January 2022 and December 2022 were selected as the research subjects. The patients' baseline data were collected, including age, gender, height, and body mass. During reconstruction operation, the S-T was measured. Considering the S-T as the dependent variable and baseline data as the independent variable, multiple linear regression analysis was used to establish a regression equation to determine the possible influencing factors of semitendinosus insertion location.@*RESULTS@#According to the selection standard, a total of 214 patients were enrolled, including 156 males and 58 females, aged (27±9) years (14-49 years), with a height of (174.7±6.8) cm (range, 160-196 cm) and a body mass of (73.43±12.35) kg (range, 53-105 kg). The S-T was (56.36±3.61) mm (range, 47-67 mm). The multiple linear regression analysis results showed that the height was positively correlated with S-T (β=0.407, SE=0.055, t=7.543, P<0.001); the regression equation was S-T=-14.701+0.407×height, R2=0.690.@*CONCLUSION@#There was a linear relationship between the height and semitendinosus insertion. The location of semitendinosus insertion estimated by the formula (S-T=-14.701+0.407×height) is reasonable, which provides a theoretical basis for rapid, accurate, and safe location of semitendinosus insertion and design of surgical incision in clinic.

Utilização de enxerto autógeno fixado por implantes / Use of autogenous graft fixed by implants / Uso de injerto autógeno fijado mediante implantes

OBJETIVO: Relatar um caso clínico em que foi utilizado enxerto autógeno na região anterior da maxila fixado com implantes. RELATO DE CASO: Paciente do gênero feminino, 46 anos, há 13 anos compareceu à Clínica de Implantodontia do Programa de Pós Graduação da UFPA tendo como queixa principal desconforto estético e dificuldade na mastigação, durante o exame clínico intraoral observou-se a ausência dos elementos 11,12, 21,22 e grande defeito ósseo em formato de U invertido. O tratamento foi dividido em duas etapas: na primeira foi realizada a correção do defeito ósseo em espessura com enxerto ósseo em bloco retirado da região mentoniana, e na segunda etapa foi realizada a correção em altura com enxerto ósseo em bloco retirado do ramo mandibular, na fixação do referido enxerto instalou-se simultaneamente os implantes. Após 13 anos, a paciente retornou para uma consulta de controle e durante a avaliação dos exames clínicos e radiológicos, observou-se que os implantes encontravam-se osseointegrados, sem sintomatologia e com ligeira perda óssea. CONCLUSÃO: Os implantes quando associados ao enxerto autógeno em bloco demonstraram-se eficazes em relação á estética e função no decorrer dos anos... (AU)

OBJECTIVE: To report a clinical case in which autogenous graft was used in the anterior region of the maxilla fixed with implants. CASE REPORT: 13years ago, a 46-year-old female patient, attended the Implantology Clinic of the UFPA Graduate Program with aesthetic discomfort and difficulty chewing as the main complaint, during the intraoral clinical examination, the absence of elements 11, 12, 21, 22 and large bone defect in inverted U format was observed. The treatment was divided into two stages: in the first one, the bone defect was correct in thickness with a block osseum graft removed from the mentionian region, and in the second stage, the correction was performed in height with block bone graft removed from the mandibular branch, and the implants were installed in the fixation of the said graft. After 13 years, the patient returned for a control consultation, and during the evaluation of clinical and radiological examinations, it was observed that the implants were Osseo integrated, without symptomatology and with slight bone loss. CONCLUSION: Implants when associated with autogenou block graft have been shown to be effective in relation to aesthetics and function over the years... (AU)

OBJETIVO: Relatar un caso clínico en que fue utilizado injerto autógeno en la región anterior de la maxila fijado con implantes. CASO CLÍNICO: Paciente del género femenino,46 años, compareció a Clínica de Implantología del Programa de Post-Graduación de la UFPA teniendo como queja principal, el malestar estético y la dificultad para masticar, durante el examen clínico intraoral, se observó la ausencia de elementos 11,12,21,22 y grande defecto óseo en formato U invertida. El tratamiento fue dividido en dos etapas: en la primera fue realizada la corrección de lo defecto óseo en espesor con material retirado de la región mentoniana, y en la segunda etapa fue realizada la corrección en altura con injerto óseo retirado de la rama mandibular, en la fijación de dicho injerto, se instalaron los implantes simultáneamente. Después de 13 años, el paciente retornó para una consulta de control, y durante la evaluación de los exámenes clínicos y radiológicos, se observó que los implantes estaban osteointegrados, sin sintomatología y con ligera pérdida ósea. CONCLUSIÓN: Los implantes cuando asociados el injerto de bloqueo autógeno se demostraron eficaces en relación con la estética y la función, en el trascurso de los años... (AU)

Are there differences in oxidative stress and inflammatory processes between the autogenous and reactive subtypes of obsessive-compulsive disorder? A controlled cross-sectional study

Objective: To date, no study has investigated whether autogenous and reactive obsessive-compulsive disorder (OCD) types are different entities in terms of oxidative stress and inflammatory processes. The aim of this study is to compare them in terms of these features. Methods: The study was conducted in subjects with reactive OCD (n=19), autogenous OCD (n=14), and a control group (n=17). All participants were non-smokers. Sociodemographic data were collected and the Yale-Brown Obsessive-Compulsive Scale (Y-BOCS), Beck Anxiety Inventory (BAI), Beck Depression Inventory (BDI), Obsessive Beliefs Questionnaire (OBQ), and Overvalued Ideas Scale (OVIS) were administered. High-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), interleukin-10 (IL-10), paraoxonase (PON1), total oxidant status (TOS), and total antioxidant status (TAS) were measured. Results: There were no significant differences in TAS, TOS, or oxidative stress index (OSI) between the OCD and control groups. PON1 and hs-CRP levels were higher in the OCD group, whereas IL-6 and IL-10 levels were lower. Comparison across the three groups revealed no differences in TAS, TOS, OSI, or PON1 levels; however, hs-CRP was significantly higher while IL-6 and IL-10 were significantly lower in the reactive group compared to controls. Conclusion: Our results show that, although inflammatory processes may play a role in OCD, the autogenous and reactive subtypes do not differ from each other in these respects. The classification of OCD into autogenous and reactive subtypes should be reevaluated.

Autotransplante dentário como ferramenta de tratamento para perdas precoces de molares / Dental self transplantation as a treatment tool for early molar loss / Auto trasplante dental como herramienta de tratamiento para la pérdida molar temprana

O transplante dentário autógeno é um procedimento cirúrgico que consiste na transposição de um elemento dentário do seu alvéolo para um outro, em um mesmo indivíduo. Esta abordagem é indicada para substituir elementos com prognóstico desfavorável ou reabilitar áreas edêntulas. O objetivo deste trabalho é apresentar as vantagens do transplante dentário autógeno frente a perdas dentárias precoces e os critérios necessários para indicação do mesmo, por meio de um relato de caso clínico no qual, uma paciente do gênero feminino, 16 anos foi submetida ao transplante do elemento 48 para o alvéolo do 47 por motivos de lesão cariosa com extensa destruição coronária com impossibilidade de tratamento clínico restaurador. Em acompanhamento pós-operatório de 01 ano os resultados demostraram continuidade do desenvolvimento radicular do elemento transplantado em seu novo alvéolo, estando este, sem mobilidade, livre de lesões periodontais e/ou endodônticas. Desta forma, conclui-se que as vantagens do transplante dentário autógeno incluem a possibilidade de reabilitação dentária de forma natural, imediata e de baixo custo comparado a tratamentos como implantes e próteses. E este método quando indicado corretamente e realizado sob os devidos cuidados pode alcançar excelentes resultados funcionais e estéticos, sendo uma opção viável para reabilitação de perdas dentárias precoces... (AU)

The Autogenous dental transplantation is a surgical procedure that consists of the transposition of a dental element from its socket to another, in the same individual. This approach is indicated to replace elements with an unfavorable prognosis or to rehabilitate edentulous areas. The objective of this paper is to present the advantages of autogenous dental transplantation against early tooth loss and the necessary criteria for its indication, through a clinical case report in which a 16-year-old female patient underwent transplantation element 48 for the alveolus of the 47 due to carious lesions with extensive coronary destruction with impossibility of restorative clinical treatment. In a 01-year postoperative follow-up, the results showed continuity of root development of the transplanted element in its new alveolus, which is without mobility, free from periodontal and/or endodontic lesions. Thus, it is concluded that the advantages of autogenous dental transplantation include the possibility of natural, immediate and low-cost dental rehabilitation compared to treatments such as implants and prostheses. And this method, when correctly indicated and performed with due care, can achieve excellent functional and esthetic results, being a viable option for the rehabilitation of early tooth loss... (AU)

El trasplante dentario autógeno es un procedimiento quirúrgico que consiste en la transposición de un elemento dentario de su alvéolo a otro, en el mismo individuo. Este abordaje está indicado para reemplazar elementos con pronóstico desfavorable o para rehabilitar áreas edéntulas. El objetivo de este trabajo es presentar las ventajas del trasplante dentario autógeno frente a la pérdida dentaria precoz y los criterios necesarios para su indicación, mediante el reporte de un caso clínico en el que se trasplantó a una paciente de sexo femenino de 16 años. 47 por lesiones cariosas con extensa destrucción coronaria con imposibilidad de tratamiento clínico restaurador. En un seguimiento postoperatorio de 01 año, los resultados mostraron continuidad del desarrollo radicular del elemento trasplantado en su nuevo alvéolo, que se encuentra sin movilidad, libre de lesiones periodontales y/o endodónticas. Así, se concluye que las ventajas del trasplante dental autógeno incluyen la posibilidad de una rehabilitación dental natural, inmediata y de bajo costo frente a tratamientos como implantes y prótesis. Y este método, correctamente indicado y realizado con el debido cuidado, puede lograr excelentes resultados funcionales y estéticos, siendo una opción viable para la rehabilitación de la pérdida dentaria temprana... (AU)

Application of digital design combined with 3D-printing technologies in dental autotransplantation / 口腔疾病防治

Objective@#To evaluate the therapeutic effect of dental autotransplantation with the application of digital design combined with 3D printing of donor tooth models and recipient alveolar fossa model preoperatively.@*Methods@# Twelve cases that could not be retained due to tooth fracture or extensive absorption of alveolar bone were recruited in the study. Cone-beam computed tomography (CBCT) data were imported into Mimics software for digital design, and the best-matched third molar was selected as the donor tooth. Replicas of the donor teeth and the recipient socket were printed out with three-dimensional (3D) printing technologies as a simulation model for recipient tooth socket preparation. During tooth autotransplantation, preparation of the recipient tooth socket and the donor tooth were guided by the 3D-printed replicas sequentially. Then, the donor tooth was implanted into the recipient tooth pocket. Patients were followed up at 3, 6 and 12 months after the operation, with CBCT examination to evaluate the status of bone reconstruction and periodontal ligaments at each time point. @*Results@#Twelve patients were transplanted with an autogenous third molar with the apical foramen completely closed. Among them, 7 patients had alveolar fossa infection before the operation, of which 1 had extensive resorption of the alveolar bone due to the infection. All 12 patients recovered well after the operation and were followed up for at least 12 months. In total, 11 caseswere successful in tooth autotransplantation with normal mastication, and 1 case had root resorption 14 months postoperation.@*Conclusion@#Digital design combined with 3D printing technology can assistin the selection of thebest-matched donor tooth and preparation of the recipient socket before tooth transplantation proceduresand reduce the extra-alveolar exposure time of the donor tooth and number of trial placementsintothe alveolar fossa. Thus, this combined strategy can effectively improve the outcome of dental autotransplantation.

Transplante dental autógeno triplo: relato de caso clínico / Triple autogenous dental transplantation: clinical case report / Triple trasplante dental autógeno: informe de caso clínico

A importância da permanência dos dentes naturais na cavidade bucal vem sendo cada dia mais reconhecida e almejada, seja pelo fator estético ou pelo funcional. Uma alternativa para a reabilitação de indivíduos com perdas dentárias ou exodontias indicadas é o transplante dental. Esta modalidade de tratamento corresponde à transferência de um órgão dental, para um alvéolo receptor. O presente trabalho relata e discute um caso de triplo transplante autógeno, utilizando terceiros molares, enfatizando as etapas cirúrgicas e demonstrando que após dois anos de preservação os resultados confirmam o sucesso deste tratamento. Anamnese, exames clínicos e radiográficos foram analisados de forma criteriosa, antes e após a cirurgia, sendo a radiografia panorâmica utilizada antes e após o ato cirúrgico e radiografias periapicais após, para acompanhamento individual das unidades transplantadas. Houve sucesso na realização dos transplantes dentários, obtendo regeneração pulpar e adaptação ao alvéolo receptor, mantendo o espaço dental com vitalidade e oclusão dentaria satisfatória. O procedimento apresentou sucesso significativo, obtendo êxito nas três unidades transplantadas, devolvendo o equilíbrio ao desenvolvimento crânio facial, comprovando sua eficácia na reabilitação oral em jovens com perdas prematuras de unidades dentárias... (AU)

The importance of the maintenance of natural teeth in the oral cavity has been increasingly recognized and desired, for the aesthetic and also the functional factor. An alternative for the rehabilitation of individuals with indicated tooth loss or extractions is dental transplantation. This type of treatment corresponds to the transfer of a dental organ to a recipient alveolar socket. The present work reports and discusses a case of triple autogenous transplantation, using third molars, emphasizing the surgical stages and demonstrating that after two years of preservation the results confirm the success of this treatment. Anamnesis, clinical and radiographic examinations were carefully analyzed, before and after surgery, with panoramic radiography used before and after surgery and periapical radiographs afterwards, for individual monitoring of transplanted units. Dental transplants were successful in achieving pulp regeneration and adaptation to the recipient alveolus, maintaining the dental space with vitality and satisfactory dental occlusion. The procedure was significantly successful, achieving success in the three transplanted units, restoring balance to the facial skull development, proving its effectiveness in oral rehabilitation in young people with premature loss of dental units... (AU)

La importancia de la permanencia de los dientes naturales en la cavidad bucal ha sido cada vez más reconocida y buscada, ya sea por motivos estéticos o funcionales. Una alternativa para la rehabilitación de personas con pérdida de dientes o extracciones recomendadas es el trasplante dental. Esta modalidad de tratamiento corresponde a la transferencia de un órgano dentario a un alvéolo receptor. Este trabajo reporta y discute un caso de triple trasplante autógeno, utilizando terceros molares, enfatizando los pasos quirúrgicos y demostrando que luego de dos años de preservación, los resultados confirman el éxito de este tratamiento. Se analizó cuidadosamente la anamnesis, los exámenes clínicos y radiográficos, antes y después de la cirugía, con radiografía panorámica antes y después de la cirugía y radiografías periapicales después, para el seguimiento individual de las unidades trasplantadas. Los trasplantes dentales fueron exitosos, logrando la regeneración pulpar y la adaptación al alvéolo receptor, manteniendo el espacio dentario con una oclusión y vitalidad dentarias satisfactorias. El procedimiento fue significativamente exitoso, teniendo éxito en las tres unidades trasplantadas, devolviendo el equilibrio al desarrollo craneofacial, demostrando su efectividad en la rehabilitación oral en jóvenes con pérdida prematura de unidades dentales... (AU)

Neo Formación Ósea de Rama y Cuerpo Mandibular Tras Uso de Injerto No Vascularizado de Cresta Ilíaca en Re-construcción Inmediata tras Resección de Ameloblastoma Folicular en Paciente de 11 años. Caso Clínico

RESUMEN: El tratamiento "gold standar" para los ameloblastomas mandibulares agresivos, especialmente en aquellas lesiones cuyos márgenes de exceresis superan los 6 cm, es la resección y reconstrucción inmediata con injerto óseo autólogo microvascularizado. Esto se basa en la necesidad de aporte sanguíneo para extensiones amplias de hueso implantado. Es necesario en estos casos una correcta estabilización y fijación del injerto mediante placas de reconstrucción (de titanio), y la ausencia de tejido sometido a radioterapia en la zona a tratar; siendo estos últimos elementos de gran importancia para el éxito quirúrgico. En el caso de pacientes pediátricos, los procesos de oseointegración y reparación ósea se ven beneficiados por el crecimiento fisiológico que conlleva la presencia de multiples factores de crecimiento. El objetivo de este reporte de caso es presentar el tratamiento de un ameloblastoma mandibular en paciente pediátrico tratado mediante resección y reconstrucción con injerto de cresta ilíaca no vascularizado de 10 cm de amplitud. Logrando neoformación ósea 3D del area reconstruida.

ABSTRACT: The gold standard for aggressive mandibular ameloblastomas, with exeresis margins higher than 6 cm, is immediate resection and reconstruction with autologous microvascularized bone graft. A controversy arises because the main reason to endorse this treatment relies on the blood supply for wide extensions of implanted bone. It is necessary in these cases to use a correct stabilization and attachment of the graft employing titanium reconstruction plates and to verify the absence of tissue undergoing radiotherapy. All of the above-mentioned elements are relevant for surgical success. In pediatric patients, the osseointegration and bone repair procedures benefit from physiological growth that entails the presence of multiple growth factors. The aim of this case report is to describe the management of mandibular ameloblastoma diagnosed in a pediatric patient and treated by resection and reconstruction with a non-vascularized 10 cm iliac crest graft thus achieving 3D bone neoformation of the reconstructed area.

Application of autogenous cartilage transplantation in correction of nasal soft-tissue triangle deformities aided by 3D technology / 中华医学美学美容杂志

Objective:To evaluate the curative effect of autogenous cartilage transplantation in the correction of nasal soft-tissue triangle deformities aided by 3D technology.Methods:From January 2016 to January 2018, 32 patients with nasal soft-tissue triangle deformities were collected, with 7 males and 25 females, aged from 18 to 32 years (mean 28.5±6.8 years). A part of the costal cartilage/nasal septum cartilage or auricular cartilage was cut as repair material, which was carved into strips, and used as nasal alar rim grafts to reconstruct the dome shape and correct the nasal soft triangle deformities. The costal cartilage or nasal septum cartilage was used to strengthen the support of the columella nasi and augmentation rhinoplasty was performed with prosthesis, and the fascia was placed on nose tip to relieve tension. The surgical results were evaluated by comparing the pre- and post-operative images; statistical analysis was conducted to compare the difference of alar cartilage angle along inside and outside implants before and after the fornix reconstruction, and the difference of maximum distance from the nostrils long shaft to the nose flange between pre- and post-operation, and so the effect of nasal soft triangular deformity correction was evaluated.Results:The alar cartilage angle of the 32 patients was (51.5±10.9)° before surgery, and decreased to (37.2±5.9)° after surgery; the difference was statistically significant ( P<0.05). The maximum distance from the nostrils long shaft to the nose flange was (3.3±0.6) mm before surgery, and it reduced to (1.9±0.7) mm after surgery; the difference was statistically significant ( P<0.05). The patients were followed up for 6 to 18 months, and the result showed that the curative effect was significant, with good appearance and natural feeling, and there were no serious complications, and the satisfactory rate was 87.5%. Conclusions:Autogenous cartilage is used to repair nasal soft-tissue triangle deformities aided by 3D technology; the nasal morphology is improved delicately, and the postoperative satisfaction of curative effect is high, which is an ideal surgical method.

Comparative study on posterior cruciate ligament reconstruction with autologous hamstring tendon and LARS artificial ligament in the treatment of KD-Ⅲ-M knee dislocation / 中国骨伤

OBJECTIVE@#To observe the curative effect of one-stage reconstruction of anterior cruciate ligament(ACL), posterior cruciate ligament (PCL) and medial collateral ligament (MCL) in patients with KD-Ⅲ-M knee injury, and to compare the operation time, hospitalization cost and curative effect after arthroscopic reconstruction of PCL with LARS artificial ligament and autogenous hamstring tendon, ACL reconstruction with autogenous hamstring tendon and MCL repair combined with limited incision.@*METHODS@#From March 2016 to January 2019, a total of 36 patients met the criteria of this study. Twenty patients in group A were treated with autogenous hamstring tendon reconstruction of ACL and PCL and repair of MCL, including 17 males and 3 females, with an average age of (34.7±9.2) years old. Sixteen patients in group B with LARS artificial ligament reconstruction of PCL, with an autogenous hamstring tendon reconstruction of PCL and MCL repair as before as group B, including 15 males and 1 female, with an average age of (36.8±8.6) years old. The operation time, hospitalization time and total hospitalization cost were compared between the two groups. The preoperative and postoperative functions of the two groups were evaluated by Hospital for Sepcial Surgery (HSS) score and Lysholm score respectively, and the curative effects were compared within and between groups.@*RESULTS@#All the patients in the two groups were followed up for at least 1 year. There were no complications such as infection and poor wound healing in both groups. There was significant difference in operation time between (120.25±9.55) min in group A and (106.63±8.85) min in group B (@*CONCLUSION@#There was no significant difference in the average hospitalization days between the two groups, but the operation time in group A was longerthan that in group B, and the hospitalization cost in group B was higher than that in group A. There was no difference in HSS score and Lysholm score before and follow-up for a certain period of time after operation.

A Clinical and Radiographic Comparative Evaluation of Self-healing Extraction Socket versus Use of Autogenous Dentin and Demineralized Freeze-Dried Bone Allograft for Socket Preservation Following Tooth Extraction

Introduction: Preservation of alveolar dimensions after tooth extraction is crucial to achieve optimal esthetic and functionalprosthodontic results. With the increasingly frequent use of dental implants to replace non-restorable teeth, preservation ofthe existing alveolus is essential to maintain adequate bone volume for placement and stabilization of the implants. The aimof the study was to clinically and radiographically compare and evaluate autogenous dentin as bone graft with demineralizedfreeze-dried bone allograft (DFDBA) and extraction socket left alone for healing.Materials and Methods: Ethical clearance was obtained for the study. A written informed consent was taken from all theparticipants. A total of 45 randomly selected adult patients were divided into three groups: (1) Extraction socket with graftmaterial placement: Autogenous dentin (natural tooth dentin) as a bone graft (15 participants). (2) Extraction socket with graftmaterial placement: DFDBA as a bone graft (15 participants). (3) Extraction socket to be left alone for healing (15 participants).The cases were examined at 3, 6, and 9 months post-intervention. For each visit, clinical and radiographic assessmentradiovisiography was done to access the bone tissue healing. The mean buccolingual bone ridge width and height werecompared both clinically and radiographically. The data collected were subjected to statistical analysis using SPSS 22.0.ANOVA and paired t-test were carried out for comparing the mean bone buccolingually on the radiograph. All P < 0.05 wereconsidered to be statistically significant.Results: The mean buccolingual ridge width measured by Vernier caliper for the I group was 7.13 ± 0.91, for II group, it was6.20 ± 0.86, and for the III group was 5.33 ± 0.61. The difference between the groups was statistically significant at 9 months.The mean height of the bone measured by radiograph showed that for I group, it was 6.33 ± 0.88, for II group, it was 6.13 ± 0.83,and for the III group, it was 5.33 ± 0.61. The difference in the mean outcome was significant between I and III as well as II andIII groups.Conclusion: The results of the grafted sites showed statistically significant difference compared to non-grafted sites.The alveolar ridge preservation shows reduction in buccolingual shrinkage. The present investigation shows thataugmenting the extraction socket with biomaterials may have the possibility to limit the buccolingual and coronalapical shrinkage.

Emergency thumb reconstruction for traumatic thumb amputation with bone and tendon from the amputated part without replantation condition / 第二军医大学学报

Objective To investigate the feasibility of emergency thumb reconstruction for traumatic thumb amputation with bone and tendon from the amputated part, when the soft tissue is severely damaged and the amputated thumb is not replantable. Methods When the soft tissues of the amputated thumb was severely damaged and replantation was not applicable, thumb reconstruction as phase one emergency management was proceeded. The skin and nail bed of the amputated thumb was removed. The bone was in situ replanted, and the tendon was repaired. The nail flap from the ipsilateral toe was grafted and the bone and tendon was wrapped around to reconstruct the thumb. Results A total of 5 patients underwent surgical treatment. All reconstructed thumbs survived with an appearance similar to the contralateral sides. The patients were followed up for 10 months on average (range, 4 to 18 months). The result of two-point discrimination test was 10.1 mm on average (range, 9.2-12.0 mm). The preserved joints possessed partial function. The average range of motion of the metacarpophalangeal joint was 69.6° (range, 20° to 90°). The range of motion of interphalangeal joint was 15° and 20° in 2 patients, respectively. No infection or bone resorption occurred. The injury was healed successfully in 4 patients, and delayed union occurred in one patient. Conclusion Complete bone and tendon from the nonreplantable amputated part combined with wrap-around flap can be used for emergency thumb reconstruction and it can achieve good clinical results.

Application of the tent bone augmentation technique in dental implants / 口腔疾病防治

@#Tent bone augmentation technology, including the tenting screw technique, autogenous cortical bone tenting technique and implant tent-pole technique, has recently been considered as a type of bone augmentation method to solve serious alveolar bone deficiency. The tenting screw technique is simple to perform and widely used in clinical practice; it can effectively increase bone in both horizontal and vertical directions in local bone defect areas with fewer complications than other techniques. The autogenous cortical bone tenting technique is mainly used for bone augmentation in large bone defect areas. The operation is relatively simple, but it needs to be combined with partial autogenous bone grafting, with high technical sensitivity and complications. The implant tent-pole technique is the most complex method and is mainly used for bone augmentation in severe alveolar ridge absorption areas of the mandible. The maximum vertical bone augmentation height can be obtained by combining this method with autogenous bone grafting, and the number of postoperative complications is relatively high. This article reviews recent research on the application of this technique to repair various alveolar bone defects in order to provide reference for clinical implant treatment.

Clinical application of autogenous dentin as a bone graft material / 口腔疾病防治

@#Autogenous dentin is a promising biological material that can be used as a substitute for autologous bone. It has been used in postextraction site preservation, maxillary sinus floor elevation, and alveolar ridge augmentation. The clinical application methods of autologous dentin have showed great diversity without uniform standard. The present article reviewed the clinical application of autogenous dentin to provide new ideas for its future development. The literature review results show that dentin materials require several preparations before transplantation, among which the demineralization is a common chemical processing method. Demineralization can enhance the osteoconductive and osteoinductive properties of dentin, but the complex and time-consuming operation process has limited its application to a certain extent. Partial demineralization may be a more appropriate choice. During transplantation, the morphology of dentin depends on the condition of the bone defect and the surgical method. Granular materials with different diameters are convenient for filling irregular defects. Block materials are conducive to maintaining the space of the reconstruction site. Hollow frame materials are slightly more complicated to process but can combine the advantages of granular and block grafts. In addition to being used alone, dentin can also be transplanted in combination with multiple biological materials. Platelet-rich plasma combined with dentin materials has shown ideal results in clinical studies. Plaster of paris and calcium phosphate ceramics have also been combined with dentin materials in animal experiments. But since they have not been applied in humans, their clinical effects require further research.

Clinical analysis of autogenous tendon reconstruction under total arthroscopy in treatment of chronic Achilles tendon rupture / 中国修复重建外科杂志

Objective: To investigate the effectiveness of autogenous tendon reconstruction under total arthroscopy in the treatment of chronic Achilles tendon rupture. Methods: Between June 2015 and June 2018, 16 patients with chronic Achilles tendon ruptures were treated by autogenous tendon reconstruction under total arthroscopy. Of the 16 patients, 11 were males and 5 were females. Their mean age was 40.7 years (range, 21-55 years). The disease duration was 14-20 months (mean, 16.4 months). Preoperative American Orthopaedic Foot and Ankle Society (AOFAS) score was 41.2±2.2 and the pain visual analogue scale (VAS) score was 7.9±1.2. MRI and B-ultrasonography examinations showed that the Achilles tendon was not continuous. The length of Achilles tendon defect was 5.0-10.3 cm, with an average of 5.8 cm. The rupture of the Achilles tendon happened on top of the insertion of the tendon in 4 cases and at the tendon-muscle belly connection in 12 cases. The operation time, intraoperative blood loss, hospital stay, and related complications were recorded. The AOFAS score and VAS score were used to evaluate the improvement of ankle joint function and pain. Results: The average operation time was 77.2 minutes (range, 60-90 minutes). The average intraoperative blood loss was 20.5 mL (range, 15-30 mL). The average hospital stay was 7.2 days (range, 5-10 days). All incisions healed by first intention. There was no skin necrosis, infection, or deep vein thrombosis. All the patients were followed up 8-18 months, with an average of 12 months; and 10 cases were followed up more than 12 months. During the follow-up, there was no Achilles tendon re-rupture, and the symptoms of pain and heel lifting failure significantly improved. MRI reexamination showed that the continuity of Achilles tendon recovered. At 1, 3, 6, and 12 months postoperatively, AOFAS scores significantly improved and VAS scores significantly reduced, except for 1 month postoperatively, the scores at other time points were superior to that before operation, the differences were significant ( P<0.05). Conclusion: Autogenous tendon reconstruction under total arthroscopy in the treatment of chronic Achilles tendon rupture has the advantages of small trauma, rapid functional recovery, and satisfactory surgical efficacy.

Management principle and clinical suggestions of osteotomy gap of opening wedge high tibial osteotomy / 中国修复重建外科杂志

Objective: To summarize the management principle and clinical suggestions of the osteotomy gap of opening wedge high tibial osteotomy (OWHTO). Methods: The related literature of the osteotomy gap of OWHTO in recent years was reviewed, summarized, and analyzed. Results: Delayed union and non-union of the osteotomy gap are main complications of OWHTO. Tomofix plate, as locking steel plate, has the characteristics of angular stability and can better maintain the stability of the osteotomy gap, promote bone healing, and avoid loss of correction. There are some treatment options for the osteotomy gap site, such as, without bone, autologous bone graft, allogeneic bone graft, bone substitute materials graft, and augment factor graft to enhance bone healing. When the osteotomy gap is less than 10 mm, it achieves a good outcome without bone graft. For the obesity, lateral hinge fracture, large osteotomy gap, or correction angle more than 10°, the bone graft should be considered. In cases whose osteotomy gap is nonunion or delayed union, the autologous bone graft is still the gold standard. When the osteotomy gap repaired with the allogeneic bone graft, it is better to choose fragmented cancellous or wedge-shaped cancellous bone, combining with the locking plate technology, also can achieve better bone union. The bone substitute material of calcium-phosphorus is used in the osteotomy gap, which has the characteristics of excellent bone conduction, good biocompatibility, and resorption, combining with the locking plate technology, which can also achieve better bone union in the osteotomy gap. The augment factors enhance the bone healing of the osteotomy gap of OWHTO is still questionable. The bone union of the osteotomy gap is also related to the size of the osteotomy gap and whether the lateral hinge is broken or not. Conclusion: No matter what type of materials for the osteotomy gap, OWHTO can improve the function and relieve pain for knee osteoarthritis. More randomized controlled trials are needed to provide evidence for clinical decision to determine which treatment option is better for the osteotomy gap of OWHTO.

Keratinized tissue augmentation techniques around dental implants / 中华口腔医学杂志

Research have indicated that inadequate keratinized tissue has a negative effect on patient oral hygiene, resulting in peri-implant inflammation. It has been recommended that an apically repositioned flap alone or in combination with autogenous soft tissue grafts can increase the width of keratinized mucosa around dental implants, which promotes long term peri-implant health. This review summarized research progress on augmentation techniques of keratinized tissue arround implants in recent years, so as to provide reference for clinical practice and research design in the future.