ABSTRACT
Objective: To study the chemical constituents of Penthorum chinense. Methods: The compounds were isolated and repeatedly purified by column chromatography over macroperous resin, polyamide, silica gel, and Sephadex LH-20, preparative TLC, and preparative RP-HPLC. Their structures were elucidated by physicochemical properties and spectral analyses. Results: Nine flavonoids were isolated and identified as 6'-hydroxy-2'-methoxy-dihydrochalcone-4'-O-β-D-glucopyranoside (1), kaempferol (2), qercetin (3), quercitrin (4), avicularin (5), afzelin (6), helicin (7), pinocembrin-7-O-[4″,6″-(S)-hexahydroxydiphenoyl]-β-D- glucoside (8), and pinocembrin-7-O-[3″-O-galloyl-4″,6″-(S)-hexahydroxydiphenoyl]-β-D-glucoside (9). Conclusion: Compound 1 is a new dihydrochalcone and named penchinoside. Compounds 5-7 are isolated from genus Penthorum Gronov. ex L.for the first time.
ABSTRACT
Objective To study the content difference of avicularin,quercitrin and quercetin in Herba Taxilli from different host plants. Methods The contents of avicularin,quercitrin and quercetin in Herba Taxilli were determined by RP-HPLC and the samples were prepared by ultrasonic extraction with methanol. The analytical column was UltImate XB-C18(250 mm×4.6 mm,5μm), the mobile phase was acetonitrile-methanol-0.1%phosphate with gradient elution,at a flow rate of 1.0 ml/min. The column tempera-ture was set at 25℃,the detection wavelength was 254 nm for avicularin and quercitrin,and 365 nm for quercetin. Results The lin-ear range of the above three ingrediets were 0.0992-1.9840(r=0.9999),0.2254-4.4580(r=0.9999)and 0.1258-2.5160μg(r=0.9997) and the average recovery rates(n=5)were 98.39%,97.08%and 98.159%,respectively. Their contents from different host plants were 0.0000-0.0398,0.3977-0.7639 and 0.0068-0.0231 mg/g in branches and 0.0167-0.1704,1.8626-11.0041,and 0.0185-0.1841 mg/g in leaves. Conclusion The method is accurate,simple and reproducible,and can be used for the quality control of Herba Taxilli.
ABSTRACT
Objective To observe the meridian tropism of Polygoni Avicularis Herba by tissue distribution of avicularin in rats.Methods Tissue distribution of avicularin in rat following a single iv administration was appointed and observed.HPLC method was established and validated for the determination of avicularin in rat tissues.Results Kidney and bladder were the most important target tissue of avicularin.Conclusion HPLC method is successfully applied to tissues distribution study of avicularin after iv administration to rats,and the results explain Polygoni Avicularis Herba on bladder tropism reasonably.