ABSTRACT
El leucograma, es uno de los estudios de rutina más solicitados en el laboratorio, la desviación de los valores de las diferentes subpoblaciones leucocitarias respecto al intervalo de referencia contribuye a la orientación sobre la posible etiología de un estado de enfermedad. El hallazgo de eosinofilia y/o basofilia absolutas, debe ser estudiado para conocer la causa subyacente. En el curso de una parasitosis, puede ser el único signo anormal o formar parte del cuadro clínico-biológico del paciente. Se presenta el caso de un niño de 4 años que cursa con una parasitosis por Echinococcus granulosus
The leukogram is one of the most requested routine studies in the laboratory; the deviation of the values of the different leukocyte subpopulations from the reference interval contributes to the orientation on the possible etiology of a disease state. The finding of absolute eosinophilia and/or basophilia must be studied to know the underlying cause. In the course of parasitosis, it may be the only abnormal sign or form part of the patient's clinical-biological state. The case of a 4-yearold boy with an Echinococcus granulosus parasite is presented
ABSTRACT
The intrauterine retention time (IURT) after fetal death can be estimated from the loss of nuclear basophilia. We therefore attempted to derive an autolysis equation to estimate IURT in experimental rat fetuses and human fetal autopsy slides. The degree of loss of nuclear basophilia in various tissues was assessed by hematoxylin and eosin (H & E) staining. Fetal rat tissues showed different rates of autolysis, allowing for the construction of an experimental autolysis curve. We also reviewed the H & E stained slides obtained from 27 human fetal autopsy cases with well-documented death intervals. The degree of autolysis in various tissues was evaluated using percentile scores (PS). Using the findings from H&E staining, we derived the equation Ln (PS/[100-PS]) = 2.62716-0.02377 x IURT. However, this equation or autolysis scores showed some limitations. Owing to the inconsistency of PS, this equation is reliably applicable only within 24 hours of intrauterine fetal death. In the fetal autopsy review, fetal hydrops, local effusion, and sepsis also contributed to accelerated autolysis.
Subject(s)
Animals , Humans , Rats , Autolysis , Autopsy , Eosine Yellowish-(YS) , Fetal Death , Fetus , Hematoxylin , Hydrops Fetalis , SepsisABSTRACT
Chronic basophilic leukemia is a rare form in chronic myeloid leukemia patients. Only limited number of reports are available. Herein, we describe a patient who presented with fatigue, weight loss, leucocytosis, prominent basophilia, and mild eosinophilia. On biopsy, bone marrow was hypercellular with marked basophils. The immunophenotype showed abnormal expression of CD7, which is suggestive of basophilic maturation. Chromosomal analysis from GTG-banded metaphases revealed Ph positivity, and fluorescence in situ hybridization (FISH) with BCR/ABL dual color, dual fusion probe showed single fusion on the der(22) chromosome and ABL/BCR fusion was deleted on the der(9) chromosome. The deletion (ABL/BCR) on der(9) may be associated with basophilia which may be also indicative of the transformation of CML to acute myeloid leukemia.
Subject(s)
Adult , Basophils/analysis , Chromosome Deletion , Humans , Leukemia, Myeloid, Acute/diagnosis , Leukemia, Myeloid, Acute/genetics , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/diagnosis , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics , MaleABSTRACT
caracterizar o perfil leucocitário de uma população socialmente carente, de acordo com as alterações mais comumente observadas. Método: analisados os dados quantitativos de 1.141 leucogramas de pacientes do Laboratório de Análises Clínicas do Instituto de Ciências Biológicas da Universidade Federal do Pará, realizados no ano de 2006. Estes foram categorizados em quatro grupos: crianças, mulheres, homens e idosos. Notou-se que as alterações nos valores absolutos em cada tipo leucocitário dificilmente estavam associadas à leucocitose ou à leucopenia, ou com alterações nos valores percentuais de um mesmo grupo leucocitário. Foi possível observar maior prevalência de leucocitoses em mulheres e em idosas, e de leucopenias em crianças e em homens. Apreciadas no total de pacientes, as alterações de maior prevalência foram a eosinofilia e a linfopenia, com maior destaque em mulheres e em crianças do sexo feminino. A linfopenia é observada principalmente no diagnóstico de deficiências nutricionais. O aumento de eosinófilos é relacionado a alergias ou a parasitoses provocadas por helmintos, o que pode resultar em outras patologias como a anemia. Foram observados 44% de casos isolados de anemia, principalmente em mulheres, e a associação mais comum da anemia foi com eosinofilia (19,1%). Conclusões: tais resultados mostram que existe uma importante relação entre carência de saúde e baixas condições sócio-econômicas
The aim is to characterize of leukocyte profile of a socially devoid population, in according with the alterations observed usually, we evaluated quantitative data of 1,141 leucograms of patients of Laboratory of Clinics Analyses of The Biological Sciences Institute in Federal University of Para, collected in 2006. These had been categorized in four groups: children, women, men, and aged. We noted that alterations in absolutes values in each type of leukocyte hardly were associates to leukocytosis or leukopenia, or with alterations in percent values in the same leukocytes group. It was possible observe the most prevalence of leukocytosis in women and aged female, and leukopenia in children and men. Appreciated in the total patients, the alterations of most prevalence were eosinophilia and lymphopenia, with evidence in women and children female. The lymphopenia is rather than noted in diagnosis of nutritious deficiencies. Eosinophils increased are related to allergies or parasitism evoked to helminthes, which can result in others pathologies like anemia. We also observed 44% of isolated cases of anemia, mainly in women, and the association most common with anemia was eosinophilia (19.1%). Such findings allow concluding that there is an important relationship between lack of health and poor social-economic conditions.
ABSTRACT
BACKGROUND: Differential diagnosis may be difficult between essential thrombocythemia (ET) and chronic myelogenous leukemia (CML) with marked thrombocytosis, mild leukocytosis, and a few immature myeloid cells in peripheral blood at onset. The aim of the present study was to analyze clinical, hematologic, and molecular features of patients with CML, mimicking ET. METHODS: Among patients from Ewha and Gachon Gil Medical Center between January 1990 and June 2001, our study group included 3 patients with Ph-positive CML with marked thrombocy-tosis (>600 X 10(9)/L) and mild leukocytosis (<20 X 10(9)/L) and 12 patients of the typical ET as a con-trol group. RESULTS: Peripheral blood basophilia (4 - 12%) and a few immature granulocytes (1 - 9%) were the characteristic features of CML with thrombocythemic onset, compared with the typical ET. There was no evidence of bone marrow eosinophilia, basophilia, or fibrosis in CML with thrombocythemic onset. CONCLUSIONS: Our study suggests that peripheral basophilia as well as the positivity of Ph chromo-somes or bcr/abl gene rearrangement can be a clue to diagnosis of CML.