ABSTRACT
Screening and identification of cell-penetrating peptides, CPP which have different functions by display technology are hot topics in recent years. Currently the most widely used display technology is phage display and mRNA display technology. This paper describes the principle and application of display technology such as phage display and mRNA display and reviews in detail the advance in study on the screening and identification of CPP by display technology in recent years.
ABSTRACT
Screening and identification of cell-penetrating peptides(CPP)which have different functions by display technolo?gy are hot topics in recent years. Currently the most widely used display technology is phage display and mRNA display technology. This paper describes the principle and application of display technology such as phage display and mRNA display and reviews in detail the advance in study on the screening and identification of CPP by display technology in recent years.
ABSTRACT
Objective To get a high affinity peptide of vascular endothelial growth factor receptor 3 (VEGFR3) as a potent carrier targeted to lymphangiogenesis of ovarian cancer via the technology of phage display. Methods Solid-phase was panned with direct VEGFR3 extracellular protein coating, then the unbound phage was washed away and the eluted phage was amplified. The positive phage clones were identified by ELISA and sequenced, and the affinity and specialty were identified by competent ELISA. Results After four-round bio-panning, the enriched positive phage clones were identified by ELISA. Eight positive phage clones were sequenced and 5 were consensus (WHGSLKQNLWWY). The short peptide displayed on screened positive phage could bind specifically to VEGFR3, and the binding could be inhibited by natural antibody VEGF-D. Conclusion The phage clone (phage-WHGSLKQNLWWY) obtained via bio-panning of peptide library has a high affinity with VEGF receptor 3. The peptide could be a potent carrier targeted to VEGFR3.