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Platelet-rich plasma and platelet-rich fibrin (PRF) are widely used in the field of stomatology. Advance-ments in preparation techniques and basic research have enabled the use of PRF derivatives in oral clinical applications. The evolution, preparation techniques, biological properties, and medical research progress of PRF derivatives are reviewed in this paper.
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Blood Platelets , Leukocytes , Oral Medicine , Platelet-Rich Fibrin , Platelet-Rich PlasmaABSTRACT
Long noncoding RNA (lncRNA) refers to RNA transcripts with more than 200 nucleotides in length, which plays regulatory roles directly in the form of RNA. Their functions are affected by their secondary structures, post transcriptional modification and so on. LncRNA can be classified to various subtypes with complicated mechanisms. In human-induced-pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs), dynamic expression of some lncRNAs is detected during the neuronal differentiation, and a series of lncRNAs associated closely with the development process of the central nervous system (CNS) are found. This review summarizes the molecular biological properties of lncRNA and their regulatory functions in the development of the CNS, which is helpful to reveal the mechanisms of the pathogenesis and progression of certain neurological diseases and results in innovation of their diagnosis and treatment.
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Objective To explore in vitro mesenchymal stem cell (MSC) transfection of lentiviral vector mediated calcitonin gene‐related peptide(CGRP) gene and its effects on biological properties of MSC .Methods MSC were isolated ,cultured and identi‐fied .MSC were infected by lentivirus encoding recombinant enhanced green fluorescent protein (EGFP) gene and CGRP (Lv‐EG‐FP‐CGRP) .The transfection efficiency was determined by the inverted fluorescence microscope and flow cytometry .The expression levels of CGRP were detected in CGRP‐modified MSC by using real‐time PCR ,immunocytochemistry and enzyme‐linked immu‐nosorbent assay (ELISA) .The proliferation ,aging and differentiation ability of MSC were evaluated by MTT ,β‐galactosidase stai‐ning and inducing differentiation respectively .Results After 48 h of MSC transfection by Lv‐EGFP‐CGRP ,EGFP/CGRP could be expressed stably .When multiplicity of infection (MOI) was 30 ,the transfection efficiency reached more than 80% .Compared with the MSC group and the MSC‐EGFP group ,the mRNA and protein expression levels of CGRP in CGRP‐modified MSC(MSC‐CGRP group) were markedly increased(all P<0 .01) .The results of MTT ,β‐galactosidase staining and inducing differentiation assay dem‐onstrated that the transfected CGRP basically had no effect on the proliferation ,aging and endotheliocyte differerntiation of MSC . Conclusion MSC is a kind of ideal genetic vector cell ,which can serve as the target cell of CGRP gene transduction for the applica‐tion of gene therapy and lays the foundation for follow‐up in vitro and vivo experiments .
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Objective To identify a aldehyde dehydrogenases positive (ALDH+) cancer stem cell subpopulation in MCF-7 cells and to investigate the proliferation and differentiation characteristics of these cells in vitro.Methods ALDH+ breast cancer stem cells were isolated from MCF-7 cells by flow cytometry and the biological property of ALDH+ breast cancer stem cells were examined by scarification test,MTT,growth curvature and Transwell migration assay.Results The ratio of CD-/low24 CD+44 cells was about 1.4 ％ in MCF-7 cells.The ratio of ALDH+ CD-/low24CD+44 cells was about 1.2 ％.The growth curvature of ALDH+ breast cancer stem cells was almost the same with that of CD-/low24 CD+44 cells.The distance between cells was obviously shorter in both CD-/low24 CD+44 cells scarification zone and ALDH+ CD-/low24 CD+44 cells scarification zone.The migration ability of CD-/low24CD+44 cells and ALDH+ CD-/low24CD+44 cells was stronger than control group cells.There were migration ability differences between CD-/low24CD+44 cells and ALDH+ CD-/low24CD+44 cells.The results of Transwell experiments were in coincidence with above results.Lots of CD-/low24CD+44 cells and ALDH+ CD-/low24CD+44 cells were through the membrane.In MTT assay,absorbance values were 1.05±0.098,1.56±0.075 and 1.67±0.032.Conclusion CD--/low24CD+44 and ALDH+ CD-/low24CD+44 breast cancer stem cell subpopulation exist in MCF-7 cells.ALDH could potentially be used as a molecular marker to identify breast cancer stem cell subpopulation.
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Objective: To explore the biological properties of keratinocytes from differently-aged healthy human beings. Methods: Keratinocytes from fetus, teenager and middle-aged groups were separated and cultured. The population doubling time (PDT) and cell growth curve in different cells were compared, and the cell cycles were analyzed by flow cytometry. Results: Circled digit one In primary culture of keratinocytes, the adherence time in middle-aged group was longer than that in fetus and teenager groups. However, all cell morphology showed no obvious differences. In subculture of keratinocytes, with donator's age increasing, time of cell adherence prolonged, passage number decreased and differences in cell morphology were obvious. Circled digit two The average PDT of keratinocytes was shorter in fetus group than in teenager and middle-aged groups. But difference in cell growth curve between different passages was not observed. Circled digit three Keratinocytes showed G2/M period in fetus group but G0/G1 period in teenager and middle-aged groups mainly. Conclusion: As age increases, the biological properties of keratinocytes change obviously.
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Objective To explore the biological properties of keratinocytes from differently-aged healthy human beings. Methods Keratinocytes from fetus, teenager and middle-aged groups were separated and cultured. The population doubling time (PDT) and cell growth curve in different cells were compared, and the cell cycles were analyzed by flow cytometry. Results ① In primary culture of keratinocytes, the adherence time in middle-aged group was longer than that in fetus and teenager groups. However, all cell morphology showed no obvioas differences. In subculture of kecatinocytes, with donator's age increasing, time of cell adherence prolonged, passage number decreused and differences in cell morphology were obrioas. ② The average PDT of keratinocytes was shorter in fetus group than in teenager and middle-aged groups. Bat difference in cell growth curve between different passages was not observed. ③ Keratinocytes showed G2/M period in fetus group but G0/G1 period in teenager and middle-aged groups mainly. Conclusion As age increases, the biological properties of keratinocytes change obviously.
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A study for processing Radix Aconiti carmichaelii in Sapa (Lao Cai province) by traditional method. Radix Aconiti was immersed in sodium and magnesium chloride solutions for 12 to 14 days until having or without slightly pungent and numb taste. Studying the chemical ingredients showed that the content of total alcaloids and aconitin were reduced. Radix Aconiti processed in different ways had different action of cardiotonic. Studying acute toxicity found that no death of mice with oral maximum dose of 320g radix aconiti per kg body weight of animals
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Biological Therapy , Pharmaceutical Preparations , Medicine, Traditional , Pharmaceutical PreparationsABSTRACT
Objective To study the role of the specific short hairpin RNA(NS-shRNA) of nucleostemin in inhibiting the expression of nucleostemin(NS) gene and evaluate the effect of NS-shRNA on differentiation antigen and biological properties of HL-60 cells,so as to explore the relationship between the biological functions of NS and acute leukemia,the potential perspective of NS gene therapy with RNA interference(RNAi).Methods HL60 cells were directly transfected NS-shRNA by using special transfection reagent.After 48 h,the inhibition rate of NS-shRNA to NS gene was detected by RTPCR,the proliferation ability of HL-60 cells was detected by MTT,the differentiation antigens were assayed by flow cytometry(FCM),the morphologic peculiarities such as cell shape,growth condition were observed,and the volume and granularity of HL-60 cells were analyzed by blood cell analyzer.Results The expression of NS gene were significantly inhibited by both two NS-shRNA,the inhibition rate were 37.82% and 71.88%,respectively.The significant inhibition effect of NS-shRNA to the proliferation of HL-60 cells existed in a time-dependent and concentration-dependent manner,and the best time was 48 h,the best concentration was 10 nmol/L.The change of differentiation antigen included increase of CD11b,CD33,CD14,CD64,HLA-DR and decrease of CD38,indicating continuous maturation of HL-60 cells to granulocytes and redifferentiation trend to monocytes.The aggregation of cells declined and the cell fragments increased.Myeloperoxidase(MPO) and ?-naphthol acetate esterase(?-NAE) activity increased after NS-shRNA-2 transfection.Furthermore,some HL-60 cells changed from round to fusiform shape even to pseudopod.The cells of small volume and karyorrhexis increased. Conclusion Through blocking the expression of NS gene,NS-shRNA can inhibit the cell proliferation and induce the differentiation of HL-60 cells,weaken malignant degree of HL-60 cells and trigger cell apoptosis.NS-shRNA is of clinical potential in gene therapy for acute leukemia.
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OBJECTIVE:To investigate the biological property of doxorubicin albumin microspheres(DR-HAS-MS) and its tissue distribution in the rats.METHODS:Pancreatin degradation method was applied to determine the biodegradation property of DR-HAS-MS as well as the correlation between the cross linking agent concentration and the degradation time and between the setting time and the degradation time.The dialysis method was applied to study the in vitro drug dissolution/ release characteristics of DR-HAS-MS vs.DR solution.DR concentrations in different tissue of rats at different time following intravenous DR HAS-MS vs.DR solution(at a dose of 2.5 mg?kg~(-1))were determined.RESULTS:The degra dation time increased with the increase of cross linking agent concentration and cross - link time.The optimal setting time was 90 min.Within 6 hours the dissolution ratio of DR from DR solution was 100%,while the releasing ratio of DR from DRHAS -MS suspension was only 80%within 168 hours;DR concentrations in the liver and the spleen in the DR HAS-MS group were significantly higher than in DR solution group(P