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1.
Chinese Journal of Biotechnology ; (12): 1-14, 2024.
Article in Chinese | WPRIM | ID: wpr-1008076

ABSTRACT

The fungal bioluminescence pathway (FBP) is a metabolic pathway responsible for the generation of bioluminescence derived from fungi. This pathway utilizes caffeic acid as the substrate, generating a high-energy intermediate, and the decomposition of which yields green fluorescence with a wavelength of approximately 520 nm. The FBP is evolutionally conserved in luminescent fungal groups. Unlike other bioluminescent systems, the FBP is particularly suitable for engineering applications in eukaryotic organisms, especially in plants. Currently, metabolically engineered luminescent plants are able to emit visible light to illuminate its surroundings, which can be visualized clearly in the dark. The fungal bioluminescent system could be explored in various applications in molecular biology, biosensors and glowing ornamental plants, and even green lighting along city streets.


Subject(s)
Luminescence , Light , Fluorescence , Eukaryota , Green Light
2.
Acta Pharmaceutica Sinica ; (12): 2922-2930, 2023.
Article in Chinese | WPRIM | ID: wpr-999051

ABSTRACT

This study focuses on the microbial quality control of the Chinese herbal decoction pieces. In view of the shortcomings of traditional culture methods such as slow detection speed and inability to detect unculturable microorganisms, a new method based on ATP bioluminescence technology combined with statistical analysis methods was established to rapidly predict and quantitatively detect the total aerobic microbial count (TAMC) and total yeast and mold count (TYMC) contaminated Bupleurum chinense DC. decoction pieces. Based on the optimized ATP bioluminesence detection system, accurate detection of pure bacterial solution of Escherichia coli, Bacillus subtilis and Staphylococcus aureus can be achieved, with detection limits of 47.86, 89.13 and 1 862.09 CFU·mL-1, respectively. The detection time was 6.5 h, and the detection cost was as low as 2 yuan/time. The upper and lower warning limits of TAMC were determined by the misjudgment rates of 10% and 20%, respectively. And the warning limit of TYMC was determined by the misjudgment rate of 20%. The proposed crossing method could quickly predict the amount of microbial contamination in Bupleurum chinense DC. decoction pieces. The constructed partial least squares regression (PLSR) model could accurately quantify the quantity of microbial contamination in Bupleurum chinense DC. decoction pieces. The optimal PLSR prediction model for TAMC had a correction coefficient (R2) of 0.826, a root mean square error of correction set (RMSEE) of 0.468 and a root mean square error of cross-validation set (RMSECV) of 0.465. The R2, RMSEE and RMSECV in the prediction model of TYMC were 0.778, 0.543 and 0.541, respectively. The aim of this study is to establish a kind of rapid detection method and prediction models for the microbial limit of traditional Chinese medicine and Chinese herbal decoction pieces, and to provide a more convenient and sensitive detection technology for the microbial quality process control of traditional Chinese medicine products.

3.
Journal of China Pharmaceutical University ; (6): 389-398, 2023.
Article in Chinese | WPRIM | ID: wpr-987658

ABSTRACT

@#Bioluminescence is a widespread phenomenon in nature, and luminescent organisms can be found both on land and in the ocean. Among them, luciferase based bioluminescence systems have been widely studied, inspiring the exploration of genetic and epigenetic aspects and the development of a series of related assays for in vivo and in vitro studies. This paper summarizes the recent developments of luciferase based bioluminescence assays in terms of bioluminescence systems, types of luciferases, and the development and application of luciferase bioluminescence assays.

4.
Chinese Journal of Biochemistry and Molecular Biology ; (12): 1390-1402, 2022.
Article in Chinese | WPRIM | ID: wpr-1015810

ABSTRACT

Chemokine signal pathways are important for the regulation of tumour metastasis. Chemokine receptors CXCR4 (C-X-C chemokine receptor type 4) and XCR1 (chemokine XC receptor 1) are involved in the metastasis of breast cancer, while the interaction between them remains unclear. Here we first identified the interaction between CXCR4 and XCR1 based on membrane protein yeast two-hybrid assays. Bioluminescence resonance energy transfer (BRET) showed that XCR1 could competitively bind to CXCR4 to form a heterodimer (P < 0.01). Results of wound healing assays via transient transfection of XCR1 and CXCR4 into HEK293 cells showed that 41.55% of the migration area rate in the co-transformation group was lower than 58.75% in the CXCR4-alone group after adding 30 nmol/L S D F-β. The co-expression of XCR1 inhibited the cellular motility, possibly mediated by the SDF-1β (stromal cell-derived factor 1)/CXCR4 signal pathway (P < 0.05). Furthermore, CXCR4 on the cell surface after co-expression of XCR1 in CXCR4-EGFP transgenic HEK293 cells was detected by flow cytometry. And the result suggested that XCR1 could accelerate the internalization of CXCR4 into the heterodimer induced by 30 nmol/L SDF-1β (P<0.05), which increased the internalization rate from 14.38% to 64.10%. Finally, the phosphorylation of Akt and ERK, which were involved in cell proliferation and migration, respectively, were examined. After 10 minutes of SDF-1β stimulation, ERK phosphorylation in the CXCR4-alone group showed a 3.59-fold increase, whereas the increase of ERK phosphorylation in the co-transfected group was only 2.08-fold. Interestingly, heterodimer formation reduced the phosphorylation level of ERK and shortened the activation time, whereas the phosphorylation level of Akt remained unchanged. Collectively, our findings revealed the hetero-dimerization of CXCR4 and XCR1 and its effects on CXCR4-mediated cellular motility, receptor internalization, and ERK pathway phosphorylation. Therefore, XCR1-targeting drugs could be candidates for cross-desensitization of CXCR4 and might represent a possible option for inhibiting breast cancer metastasis.

5.
Acta Pharmaceutica Sinica B ; (6): 637-650, 2022.
Article in English | WPRIM | ID: wpr-929316

ABSTRACT

Receptor activity-modulating proteins (RAMPs) are accessory molecules that form complexes with specific G protein-coupled receptors (GPCRs) and modulate their functions. It is established that RAMP interacts with the glucagon receptor family of GPCRs but the underlying mechanism is poorly understood. In this study, we used a bioluminescence resonance energy transfer (BRET) approach to comprehensively investigate such interactions. In conjunction with cAMP accumulation, Gα q activation and β-arrestin1/2 recruitment assays, we not only verified the GPCR-RAMP pairs previously reported, but also identified new patterns of GPCR-RAMP interaction. While RAMP1 was able to modify the three signaling events elicited by both glucagon receptor (GCGR) and glucagon-like peptide-1 receptor (GLP-1R), and RAMP2 mainly affected β-arrestin1/2 recruitment by GCGR, GLP-1R and glucagon-like peptide-2 receptor, RAMP3 showed a widespread negative impact on all the family members except for growth hormone-releasing hormone receptor covering the three pathways. Our results suggest that RAMP modulates both G protein dependent and independent signal transduction among the glucagon receptor family members in a receptor-specific manner. Mapping such interactions provides new insights into the role of RAMP in ligand recognition and receptor activation.

6.
Journal of Pharmaceutical Analysis ; (6): 253-262, 2020.
Article in Chinese | WPRIM | ID: wpr-824003

ABSTRACT

In traditional Chinese medicine herbs (TCM), including Radix Salviae Miltiorrhizae (Danshen), Radix Puerariae Lobatae (Gegen), Radix Angelicae Sinensis (Danggui), and Rhizoma Chuanxiong (Chuanxiong) are widely used for the prevention and treatment of cardiovascular diseases and also often co-administered with Western drugs as a part of integrative medicine practice. Carboxylesterase 1 (CES1) plays a pivotal role in the metabolisms of pro-drugs. Since (S)-2-(2-(6-dimethylamino)-benzothiazole)-4,5-dihydro-thiazole-4-carboxylate (NLMe) has recently been identified by us as a selective CES1 bioluminescent sensor, we developed a rapid method using this substrate for the direct measurement of CES1 activity in rats. This bioluminescence assay was applied to determine CES1 activity in rat tissues after a two-week oral administration of each of the four herbs noted above. The results demonstrated the presence of CES1 enzyme in rat blood and all tested tissues with much higher enzyme activity in the blood, liver, kidney and heart than that in the small intestine, spleen, lung, pancreas, brain and stomach. In addition, the four herbs showed tissue-specific effects on rat CES1 expression. Based on the CES1 biodistribution and its changes after treatment in rats, the possibility that Danshen, Gegen and Danggui might alter CES1 ac-tivities in human blood and kidney should be considered. In summary, a selective and sensitive biolu-minescence assay was developed to rapidly evaluate CES1 activity and the effects of orally administered TCMs in rats.

7.
Rev. biol. trop ; 68(4)2020.
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1507740

ABSTRACT

Introducción: El complejo enzimático emisor de luz de las bacterias luminiscentes es una poderosa herramienta bioquímica, con una amplia variedad de aplicaciones, incluyendo el control de la calidad ambiental. Objetivos: Identificar taxonómicamente dos bacterias luminiscentes de las aguas de la plataforma cubana, así como seleccionar los medios de cultivo que favorezcan su crecimiento y luminiscencia. Métodos: La identificación taxonómica de las bacterias luminiscentes se llevó a cabo utilizando métodos tradicionales y moleculares. Cuatro medios de cultivo (LM, Boss, Chalk, ZoBell) fueron evaluados en función de la tasa de crecimiento específico (μ) y la luminiscencia utilizando un espectrofotómetro Genesys 10UV y un espectro fluorómetro Shimadzu RF-5301pc, respectivamente. Resultados: La caracterización bioquímica y fisiológica de los aislamientos de CBM-976 y CBM-992 mostró similitudes con las especies de Vibrio harveyi. El análisis del posicionamiento taxonómico confirmó una alta correspondencia con las cepas de V. harveyi aisladas de entornos acuáticos, utilizando secuencias parciales de los genes 16S rRNA, gyrB y pyrH. Se seleccionaron los medios de cultivo LM y ZoBell por tener una alta tasa de crecimiento específico de las cepas CBM-976 y CBM-992; así como por mostrar altos valores de luminiscencia. Los resultados permitirán profundizar en la caracterización fisiológica y son el punto de partida para el desarrollo de métodos de detección de contaminantes. Conclusiones: La combinación de las características fisiológicas y bioquímicas, así como las técnicas de biología molecular contribuyeron a determinar la posición taxonómica de las cepas CBM-976 y CBM-992 aisladas de las aguas marinas cubanas como Vibrio harveyi. Además, se seleccionaron los medios de cultivo LM y ZoBell como los más adecuados para el crecimiento y la emisión de luminiscencia de ambas cepas.


Introduction: The light-emitting enzyme complex of luminescent bacteria is a powerful biochemical tool, with a wide variety of applications including environmental quality monitoring. Objectives: To identify taxonomically two luminescent bacteria from Cuban shelf waters, as well as select the culture media that favor their growth and luminescence. Methods: The taxonomic location of the luminescent bacteria was carried out using traditional and molecular methods. Four culture media (LM, Boss, Chalk, ZoBell) were evaluated as a function of specific growth rate (μ) and luminescence, using a Genesys 10UV spectrophotometer and a Shimadzu RF-5301pc spectrofluorometer, respectively. Results: Biochemical and physiological characterization of CBM-976 and CBM-992 isolates showed similarities with Vibrio harveyi species. Phylogenetic positioning analysis confirmed a high correspondence with V. harveyi strains isolated from aquatic environments, using partial sequences of 16S rRNA, gyrB and pyrH genes. LM and ZoBell culture media were selected for having a high specific growth rate of CBM-976 and CBM-992 strains, as well as for showing high luminescence values. The results will allow deepening the physiological characterization and are the starting point for the development of contaminant detection methods. Conclusions: The rational combination of physiological and biochemical characteristics, as well as the molecular approach, contributed to determine the taxonomic position of CBM-976 and CBM-992 strains isolated from Cuban marine waters as Vibrio harveyi. Furthermore, LM and ZoBell culture media were selected as the most suitable for growth and luminescence emission for both strains.

8.
Article | IMSEAR | ID: sea-209780

ABSTRACT

Paralytic Shellfish Poison (PSP) are the most harmful neurotoxins create a serious public health problem. It is important to assess PSP in Shellfish destined for human consumption. However, recommended methods have some limitations for example in the case of Mouse Bioassay (MBA) showed a low sensitivity and reproducibility, and undesirability for ethical reasons; while physico-chemical techniques rest expensive and time-consuming. The main objective of this study, after discovered that PSP inhibited the luminescence of Vibrio fischeri, was the quantification of PSP by using Bioluminescence Inhibition Assay (BIA), and comparing the results obtained with those determined by MBA and LC-MS. Bivalve used were collected from Corniche Martil, Kabila and Oued Laou, along the Mediterranean coast of Morocco in Mars-2015. Results showed a weak correlation between LC-MS and MBA with r = 0.11, while, the correlation between LC-MS and BIA was very strong with r = 0.97, which suggests that, BIA could offer an interesting additional assessment of PSP risk. In addition, after seen its rapidity, ease, reliability, sensitivity, reproducibility and cost effectiveness, it would be eligible to use for monitoring in surveillance programs.

9.
Chinese Journal of Medical Imaging Technology ; (12): 1622-1626, 2019.
Article in Chinese | WPRIM | ID: wpr-861163

ABSTRACT

Objective: To explore the ability of combining bioluminescence imaging (BLI) and MRI to make real-time and non-invasive tracer of mesenchymal stem cells (MSCs) transplanted into damaged liver of rats; and to study the functional repair effect of transplanted MSCs on injured liver in rats. Methods: Rat model with acute liver injury were transplanted the MSCs of labeled luciferase and SPION. Rat plasma aminotransferase levels and liver pathology change were analyzed, and the repair effect of MSCs on liver function injury were observed. The time course of MSCs accumulation in rat liver was observed by BLI and MRI. In situ immunohistochemical staining of rat liver tissue sections was performed. Results: Plasma aspartate aminotransferase and alanine aminotransferase levels were significantly lower on days 5, 9 and 10 after transplantation than in the control group (all P<0.05). On the first day after the transplantation of MSCs, the bioluminescence intensity in the liver area of rats was significantly enhanced, and the T2*WI standardized signal intensity was significantly reduced, and then gradually returned to the pre-transplantation level 10 days after the transplantation. The number of luciferase-positive MSCs decreased with the decrease of bioluminescence intensity. Conclusion: Transplantation of MSCs has a good repair effect on the damaged liver function of rats. In addition, BLI combined with MRI is used for real-time and non-invasive tracing of MSCs transplanted into the injured liver of rats.

10.
Int. j. odontostomatol. (Print) ; 12(2): 160-168, jun. 2018. tab, graf
Article in Spanish | LILACS | ID: biblio-954259

ABSTRACT

RESUMEN: El estudio fue realizado en 40 estudiantes de ambos sexos, que cursaban 2° año el año 2017 de la carrera de Odontología, en estudiantes de la Universidad de La Frontera. El objetivo fue evaluar la efectividad de la técnica de Lavado de Manos Clínico (LMC) demostrando el grado de eficiencia, mediante Bioluminiscencia. La técnica que permite una medición cuantitativa rápida de los residuos orgánicos de superficie, por la cuantificación de cantidad de Adenosín Trifosfato (ATP). Empleándose un Luminómetro, 3M™ Clean-Trace™, se programó la medición de contaminación en dos puntos claves, región interdigital e hiponiquio, en ambas manos, por medio de la lectura de Unidades Relativas de Luz (URL). Se analizó la contaminación previa a la técnica de LMC y posterior al desarrollo de la técnica, en las regiones indicadas, por medio de Hisopado v/s aprobación/rechazo considerando como Aprobado <250 URL y Rechazado >251 URL., nivel de contaminación v/s sexo, y distribución de aprobación/rechazo tanto antes y después de realizada la técnica de LMC. El muestreo previo al LMC determinó en todas las localizaciones muestreadas existen rangos de aprobación/ rechazo entre 35-42,5 % / 57,5-65 %, encontrándose la mayor contaminación en ambas manos en hiponiquio. Posteriormente de realizada la técnica de LMC el rango de corte de aprobación/rechazo fue de 80-90 % / 10-20 %, el porcentaje más alto se pesquisó en mano izquierda hiponiquio. Para el análisis estadístico se utilizó el programa estadístico SPSS Statistics for Windows, análisis descriptivo, prueba t- test, prueba de Levene. Un valor de p < 0,05 para significancia. Obteniéndose diferencias significativas en análisis del valor la media, según zonas de muestreo y rangos de aprobación/rechazo en URL, que varían entre p=0,000 y p=0,015, que se presentó en mano izquierda zona interdigital posterior al LMC. Según la media concluimos que las regiones hisopadas tanto previas y posteriores a la ejecución del LMC en hiponiquio la piel está más contaminada, que es paradojal ya que la técnica incorpora un acento en esta localización, tiene un mejor acceso y contacto con jabón y agua.


SUMMARY: The study was conducted in 40 undergraduate students of both sexes, who were in the 2nd year of the 2017 year enrolled in the Undergraduate Dentistry Program of the Universidad de La Frontera. The objective was to evaluate the effectiveness of a Clinical Hand Washing technique (CML), demonstrating the degree of efficiency and implementing improvement behaviors of the technique using Bioluminescence. This technique allows a rapid quantitative measurement of organic residues on a surface by quantifying the amount of Adenosine Triphosphate (ATP). For that purpose, a Luminometer, 3M™ Clean-Trace™, was used to obtain the contamination level in two key points, Interdigital Region and Hyponychium, of both hands by quantification of Relative Light Units (URL). Hand contamination prior to CML and after the development of the technique was tested in the Interdigital and Hyponychium regions of both hands, through surface ATP swab considering as Approved <250 URL and Rejected > 251 URL. Also, the level of contamination v/s sex, and approval/rejection distribution before and after the CML were tested. The approval/rejection range for pre-CML sampling at all selected locations was 35-42.5 % / 57.5-65 %, fin ding the highest contamination rate in Hyponychium in both hands. After performing the CML we obtained 90 % of approval in all sites, with a 10 % rejection rate; the highest percentage was surveyed on left hand Hyponychium. Statistical analysis was performed using SPSS Statistics for Windows, a descriptive analysis, test t-test for independent samples, test of homogeneity of Levene variances. A p value <0.05 was established for statistical significance. There were significant differences in analysis of the mean value, according to sampling zones and URL approval / rejection ranges, ranging from p = 0.000 to p = 0.015 (Interdigital region of left hand, post-LA TÉCNICA DE LMC). The left hand presented greater contamination in both swab areas both before and after the execution of CML. The most contaminated region was the Hyponychium, which was paradoxical, because the technique emphasizes the hygiene of this location and has better access and contact with soap and water.


Subject(s)
Humans , Male , Female , Dentistry/methods , Bioluminescence Resonance Energy Transfer Techniques , Adenosine Triphosphate , Hand Disinfection , Cross-Sectional Studies
11.
Acta Pharmaceutica Sinica ; (12): 84-89, 2018.
Article in Chinese | WPRIM | ID: wpr-779848

ABSTRACT

This study was designed to explore the mechanism of Coix seed oil (Coix) impact on the drug resistance, bioluminescence imaging (BLI) and the efflux of D-luciferin potassium salt, the substrate of ABC transporters, in doxorubicin-resistant breast cancer cells. Multidrug resistance (MDR) gene and protein expression were analyzed in the cells by q-PCR and Western blot. First, in order to investigate the effect of the efflux function by ABC protein, a cell line with overexpressed luciferase was established in MCF-7 cell line. BLI was used to monitor the efflux kinetics of D-luciferin potassium salt before and after Coix treament. The results showed that the efflux of D-fluorescein potassium from MCF-7/DOXFluc was lessened when pretreated with Coix, which means that Coix may decrease the efflux of other chemotherapies using ABC transporters. Both of the results of q-PCR and Western blot showed that gene and protein expression of ABC transporters such as ABCG2, ABCC1 and ABCB1 were down-regulated by Coix treatment. The efficacy of Coix reversing MDR was verified with the chemotherapy medication doxorubicin (DOX). MTT assay showed that Coix increased the inhibitory effect of DOX on proliferation of MCF-7/DOX, and the optimal combination of ratio was 25 times that of DOX. The results suggest that Coix may reverse MDR of the substrate of ABC transporters from two aspects, one is to cut down the ABC protein efflux function, and the other is to decrease the quantity of ABC gene and protein expression.

12.
Chinese Journal of Infection Control ; (4): 260-263, 2018.
Article in Chinese | WPRIM | ID: wpr-701605

ABSTRACT

Objective To investigate the cleanliness status of personal digital assistant(PDA)surfaces, and observe the effect of disposable antiseptic wipes on the cleaning and disinfection of PDA surface.Methods From January to March 2016, 83 daily used handheld PDA in 50 clinical wards of a hospital were as control group, 83 PDA disinfected by disposable antiseptic wipes(CaviWipes)and used in the same environment of the ward at the same time were selected as the intervention group, cleanliness status of PDA surfaces of two groups were detected by adenosine triphosphate(ATP)bioluminescence assay.Results Taken a cut-off value≤100 RLU/100 cm2 as qualified standard of cleanliness, none of PDA in control group was qualified with ATP bioluminescence reading ranged from 189 to 3 379 RLU;after disinfection with CaviWipes, the qualified rate of intervention group was 90.4%(n=75), ATP detection value of PDA surface in all departments were significantly lower than control group(median:[18-28] RLU vs[290-339]RLU, all P<0.05).Conclusion Microbial contamination on PDA used in hospital wards is common, antiseptic wipes containing quaternary ammonium salt can be used for cleaning and disinfecting surface of PDA.

13.
Chinese Traditional Patent Medicine ; (12): 284-290, 2018.
Article in Chinese | WPRIM | ID: wpr-710180

ABSTRACT

AIM To study the effects of Elemene Injection (ELE) on the kinetics of intracellular transport of Gefitinib (GEF) in PC-9/GR cells and to probe the role of ELE in reversing oncological multidrug resistance.METHODS The intracellular pharmacokinetic behavior of D-luciferin potassium salt,a substrate of an ATP-binding cassette (ABC) protein,was investigated in PC-9/GRFluc cells using real-time bioluminescence imaging.The resistance of PC-9/GR cells to GEF was determined by MTT assay.Compusyn software was used to analyze the synergistic effect of GEF and ELE,and HPLC to detect the uptake of GEF in PC-9/GR cells.RESULTS The respective GEF IC50 values of 0.01 μg/mL in PC-9 cells and 1.50 μg/mL in PC-9/GR cells revealed the 150 times drug resistance of PC-9/GR to PC-9 cells.The significantly enhanced intracellular fluorescence intensity of D-fluorescein potassium salt by the intervention of ELE also indicated remarkable GEF uptake increase in PC-9/GR cell line (P < 0.05) due to the synergistic result.CONCLUSION Partly as the mechanism in reversing oncological multidrug resistance,ELE,a booster for the fluorescence intensity of D-luciferin potassium salt,promotes cellular uptake of GEF by inhibiting efflux function of ABC proteins.

14.
Chinese Journal of Radiation Oncology ; (6): 222-225, 2018.
Article in Chinese | WPRIM | ID: wpr-708172

ABSTRACT

The theory and application of small animal radiotherapy models is critical for the research and development of radiobiology and clinical radiotherapy.Considering universality and cost effects,mouse models are widely used to explore the radiobiological mechanisms of cancerous and normal tissues.In recent years,there has been tremendous progress in image-guided stereotactic radiotherapy equipment for small animals,which could simulate the human radiotherapy process.This article introduces stereotactic radiotherapy systems for small animals guided by different imaging modalities,such as cone-beam computed tomography and magnetic resonance imaging,and then reviews small animal fluorescence imaging technology and summarizes the application of different bioluminescence and fluorescence imaging equipment in small animal imaging systems.Finally,we put forward the prospect of optimization direction of radiotherapy equipment for small animals in future.

15.
Rev. chil. infectol ; 35(1): 88-90, 2018. graf
Article in Spanish | LILACS | ID: biblio-1042644

ABSTRACT

Resumen Introducción La atención odontológica se ha transformado en un desafío para los programas de prevención y control de infecciones asociadas a la atención de salud (IAAS), dado que el ambiente, entre otros factores, juega un rol importante en la cadena de transmisión. Método Se realizó un programa de intervención en el Servicio de Dental del Hospital Militar de Santiago, entre los años 2014-2015, que contempló tres etapas: etapa diagnóstica, de intervención y de evaluación. Objetivo Mejorar la seguridad de las superficies críticas involucradas en la atención dental. Resultados Durante la etapa diagnóstica se constató que el proceso de limpieza y desinfección era deficiente. La superficie crítica más contaminada fue la unidad porta-instrumental, luego el área limpia y la manilla de la lámpara. Las superficies que redujeron significativamente su contaminación, posterior a la intervención, fueron el área limpia y la unidad porta-instrumental. Conclusiones La capacitación en relación a los procesos de limpieza y desinfección de superficies y del equipamiento dental es una de las estrategias costo-eficientes en la prevención de las IAAS, sencilla y fácil de aplicar.


Introduction Dental care has become a challenge for healthcare associated infection prevention programs, since the environment, within other factors, plays an important role in the transmission chain. Materials and Methods An intervention program was designed for the Dental Unit of Hospital Militar de Santiago, between years 2014 and 2015. The program contemplated 3 stages: diagnostic, intervention and evaluation stage. Objective To improve the safety of critical surfaces involved in dental healthcare. Results During the diagnostic stage, the cleaning and disinfection process was found to be deficient. The most contaminated critical surface was the instrument holder unit, then the clean area and lamp handle. The surfaces that significantly reduced their contamination, after the intervention, were the clean area and the instrument carrier unit. Conclusion Training in the processes of cleaning and disinfecting surfaces and dental equipment is one of the cost-effective strategies in preventing healthcare-associated infections (HCAI), with simple and easy-to-apply methods.


Subject(s)
Disinfection/methods , Cross Infection/prevention & control , Equipment Contamination/prevention & control , Dental Equipment/microbiology , Dental Health Services , Surface Properties , Program Evaluation , Adenosine Triphosphate/analysis , Disinfection/standards , Reproducibility of Results , Infection Control/methods , Infection Control/standards , Luminescent Measurements
16.
Ciênc. rural (Online) ; 48(2): e20161111, 2018. tab, graf
Article in English | LILACS | ID: biblio-1045065

ABSTRACT

ABSTRACT: The quorum sensing phenomenon is a process of intra- and inter-species microbial communication involving the production and detection of extracellular signaling molecules. The autoinducer AI-2 has been proposed to serve as a 'universal signal' for interspecies communication. This study aimed to evaluate the capability of Enterococcus faecium, Enterococcus faecalis, and Bacillus cereus strains isolated from ricotta processing to produce quorum sensing signalling molecules (AI-2). The strains were evaluated for the presence of the luxS gene using the polymerase chain reaction. AI-2 quorum sensing signalling molecules were measured in relative light units (RLUs) using a luminometer. A total of 74% of E. faecium, 91% of E. faecalis, and 95% of B. cereus isolates were positive for luxS gene. In addition, the induced bioluminescence in Vibrio harveyi BB170 was observed in all strains, indicating the presence of the AI-2 autoinducer.


RESUMO: O fenômeno quorum sensing corresponde a um processo de comunicação intra e interespécies microbianas e é mediado por sinais químicos extracelulares, denominados moléculas sinalizadoras ou auto indutoras (AI). A molécula AI2 está envolvida na comunicação interespécies, denominada sistema "universal" de comunicação. Este estudo teve como objetivo avaliar a capacidade de Enterococcus faecium, Enterococcus faecalis e Bacillus cereus isolados do processamento de ricota em produzir moléculas sinalizadoras de Quorum sensing (AI-2). Os isolados foram avaliados quanto à presença do gene luxS utilizando a reação em cadeia da polimerase (PCR). As moléculas sinalizadoras (AI-2) foram medidas em unidades relativas de luz (RLU) através de um luminômetro. Um total de 74% dos isolados de E. faecium, 91% de E. faecalis e 95% de B. cereus foram positivos para o gene luxS. Além disso, todos os isolados apresentaram capacidade de induzir o fenômeno de bioluminescência em Vibrio harveyi BB170, indicando a presença de auto indutores AI-2.

17.
An. acad. bras. ciênc ; 90(1,supl.1): 663-679, 2018. graf
Article in English | LILACS | ID: biblio-886935

ABSTRACT

ABSTRACT Bioluminescence - visible and cold light emission by living organisms - is a worldwide phenomenon, reported in terrestrial and marine environments since ancient times. Light emission from microorganisms, fungi, plants and animals may have arisen as an evolutionary response against oxygen toxicity and was appropriated for sexual attraction, predation, aposematism, and camouflage. Light emission results from the oxidation of a substrate, luciferin, by molecular oxygen, catalyzed by a luciferase, producing oxyluciferin in the excited singlet state, which decays to the ground state by fluorescence emission. Brazilian Atlantic forests and Cerrados are rich in luminescent beetles, which produce the same luciferin but slightly mutated luciferases, which result in distinct color emissions from green to red depending on the species. This review focuses on chemical and biological aspects of Brazilian luminescent beetles (Coleoptera) belonging to the Lampyridae (fireflies), Elateridae (click-beetles), and Phengodidae (railroad-worms) families. The ATP-dependent mechanism of bioluminescence, the role of luciferase tuning the color of light emission, the "luminous termite mounds" in Central Brazil, the cooperative roles of luciferase and superoxide dismutase against oxygen toxicity, and the hypothesis on the evolutionary origin of luciferases are highlighted. Finally, we point out analytical uses of beetle bioluminescence for biological, clinical, environmental, and industrial samples.


Subject(s)
Animals , Male , Female , Coleoptera/physiology , Coleoptera/chemistry , Luminescence , Luciferases/metabolism , Behavior, Animal , Brazil , Forests , Evolution, Molecular , Luciferases/chemistry
18.
Military Medical Sciences ; (12): 269-272,277, 2017.
Article in Chinese | WPRIM | ID: wpr-621435

ABSTRACT

Objective To construct a mouse model for real-time,noninvasive and specific monitoring of inflammation activation in hepatic tissues.Methods An inflammation reporter gene was targeted to the liver by hydrodynamic gene delivery technology.Bioluminescence imaging was used to detect the firefly luciferase(Fluc) expression in the mouse liver after inflammatory stimulation.Besides,the relevance between the light intensity and inflammation level was also intensively investigated.Results pIL-6-Fluc was successfully delivered to the liver.The hydrodynamic gene delivery could cause a transient liver injury that could return normal in 5 to 7 days.The expression of pIL-6-Fluc could be induced by lipopolysaccharides(LPS) treatment with an about (46.80±13.35) fold increase at the peak value,which was significantly higher than that detected by ELISA [(4.09±0.96)fold].Conclusion An inflammation reporter mouse model is constructed in this study by hydrodynamic gene transfection,allowing noninvasive monitoring of inflammation activation specifically in hepatic tissues.The reporter model is capable of monitoring inflammation activation with a sensitivity higher than that of ELISA.

19.
Chinese Journal of Clinical Oncology ; (24): 583-588, 2017.
Article in Chinese | WPRIM | ID: wpr-620781

ABSTRACT

Objective:To study the signal enhancement of lung adenocarcinoma nude mice after injection of immunomagnetic bead solution (magnetic beads conjugated with monoclonal antibody NJ001) in micro-CT scan. Methods:The models of lung adenocarcino-ma nude mice were established by injecting SPC-A1-luc cells through the tail vein and were validated by bioluminescence imaging (BLI). The nude mice were divided into three groups: physiological saline group, bare magnetic bead group, and immunomagnetic bead group. Three groups of nude mice were injected with physiological saline, 750 nm bare magnetic bead solution, and immuno-magnetic bead solution via the tail vein every week, and micro-CT scan was taken before and 4 h after injection. Immunohistochemis-try (IHC) was used to detect the expression of antigen SP70 in tumor tissues. Results:The tumor was detected in the immunomagnetic bead group at the fourth week, whereas in the physiological saline and bare magnetic bead groups, the tumor was undetectable until the sixth week. The tumor intensities detected at the sixth week by micro-CT scan in the physiological saline, bare magnetic bead, and immunomagnetic bead groups were 59.05 ± 0.66, 60.69 ± 0.55, and 58.25 ± 0.32 before injection and 60.30 ± 1.83, 61.05 ± 0.68, and 67.41±3.82 after injection, respectively. Compared with the tumor intensities before injection, they significantly increased after injec-tion in the immunomagnetic bead group;the difference was statistically significant (P=0.0079). By contrast, no statistical significance was observed in the tumor intensities before and after injection in the physiological saline and bare magnetic bead groups (P=0.1867 and P=0.3839, respectively). Conclusion:The immunomagnetic beads had enhanced effect on micro-CT scan of lung adenocarcinoma nude mouse models.

20.
Chinese Medical Equipment Journal ; (6): 107-109, 2017.
Article in Chinese | WPRIM | ID: wpr-618916

ABSTRACT

Objective To explore the feasibility of applying ATP bioluminescence technology to disinfection quality monitoring of the flexible endoscope.Methods Totally 30 flexible endoscopes used repeatedly from October 2014 to March 2015 were randomly selected,and had the disinfection quality monitored by ATP bioluminescence technology and bacterial culture method respectively.Parallel comparison was carried out to evaluate the feasibility of applying ATP bioluminescence technology to disinfection quality monitoring of the flexible endoscope.Results ATP bioluminescence technology showed that the qualification rate of the disinfection was 93.3%,and bacterial culture method found it was 96.7%.The two methods proved the outer surface of the endoscope had the disinfection acceptable while the biopsy hole and intracavity not.There was no significant correlation between the two methods while high consistency between the detection results by the two methods.Conclusion ATP bioluminescence technology can be used for preliminary screening in field,instant and daily monitoring of the medical flexible endoscope,which assists bacterial culture method in disinfection quality monitoring of the flexible endoscope.

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