ABSTRACT
Starting from participating the high-level professional competition, our school has built a talent training system with the spirit of "biomaker" and an innovative practical ability training system. Such system takes the interest of student as the starting point, and relies on the strong scientific research and teaching infrastructure. The programme gives full play to students' initiatives and enhances the scientific research literacy and comprehensive ability of undergraduates majoring in biotechnology. It is an effective exploration of the traditional university education model and meets the urgent demand for innovative talents training in the era of rapid development of life sciences.
Subject(s)
Humans , Biological Science Disciplines , Biotechnology , Genetic Engineering , Students , UniversitiesABSTRACT
Objective: To observe the effect of Wuzhuyu Decoction on the serum metabolic profile of the model rats with deficiency cold, and explore its possible mechanism for treating deficiency cold and vomiting syndrome. Methods: A rat model of deficiency cold and vomiting was prepared by a composite method (Rhei Radix et Rhizoma + cisplatin). The high-throughput metabolome data of the serum of rats with deficiency-cold and vomiting was obtained by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS), and multivariate analysis combinated with database retrieval was used to analyzed the endogenous metabolites in rat serum from different groups and discover differential metabolites. Besides, metabolites were analyzed by Metabo Analyst software for possible metabolic pathways. Results: The serum metabolic spectrum of control group and model group were completely separated. The Wuzhuyu Decoction group was close to the control group, indicating that the horizontal model of endogenous material changes was successfully replicated, and Wuzhuyu Decoction could interfere with the symptoms of deficiency cold and vomiting in rats, suggesting that the rat body had a tendency to return to normal state. In total, modular analysis of 35 serum differential metabolites, and deficiency cold and vomiting syndrome was related to abnormal lipid metabolism and amino acid metabolism pathways and Wuzhuyu Decoction may have a regulatory effect on its pathways; Metabolites revealed close relationship among seven modules; Pyroglutamic acid, arachidonic acid, serotonin, isocitric acid, triglyceride and other biological targets can thus be used as biomarkers of deficiency cold and vomiting. Conclusion: Wuzhuyu Decoction can improve the physiological characteristics of the model of deficiency-cold and vomiting. The mechanism may be related to the regulation of abnormal amino acid metabolism, energy metabolism, and lipid metabolism in rats with deficiency-cold vomiting.
ABSTRACT
Sepsis is a complex systemic inflammatory response syndrome caused by infection.Sepsis can deteriorate to severe sepsis,septic shock,and multiple organ dysfunction syndrome.Due to the use of antimicrobial agents,fluid resuscitation and the developments of all kinds of support life,the treatment of sepsis has greatly improved over the past years.However,the mortality rates of sepsis still remain high.Rapid,accurate and early diagnosis of sepsis is a key factor to lower the high mortality rate of sepsis.Recently,it has been found that some biomarkers are closely associated with early diagnosis of sepsis and are helpful for the treatment.These biomarkers for early diagnosis include presepsin(sCD14-subtype),neutrophil CD64,soluble triggering receptor expressed on myeloid cells-1 (sTREM-1),soluble CD163,microRNAs,copeptin,and so on.Examining these biomarkers alone or in combination will bring new opportunities for the early diagnosis of sepsis,and hopefully provide new ideas for the treatment of sepsis.This article reviews the progress on the current emerging biomarkers for early diagnosis of sepsis.
ABSTRACT
Metabolomics is one of the branches of systems biology,which employs nuclear magnetic resonance and mass spectrometry technologies to detect the abnormal metabolites from a variety of body fluids. Study of various molecules and their functions may help to find the disease-related early metabolic marker clusters,understand the molecular mechanism of pathogenesis,which provides broad prospects for the early diagnosis and individualized treatment of malignant tumors.