ABSTRACT
AIM To study the boosting effect of Maximin 2 on expression of HLA DR and HLA ABC by cultured bladder cancer cell lines and compare with TNF ??IFN ?. METHODS Cell culture and FCM were used to dectect the effects of Maximin 2,TNF and IFN on the cell growth of three types of bladder cancer cell lines and HLA DR and HLA ABC expression under the IC 50 culture concentration. RESULTS Maximin 2 had show inhibition to cells growth under the small dosage. TNF ??IFN ? also had the effect on restrain all 3 cell lines growth. The expression of HLA DR was not any change in all 3 cell lines after being stimulated by Maximin 2?TNF ? and IFN ?, Maximin 2 and TNF ? can not change HLA ABC expression, IFN ? can up regulate HLA ABC expression. CONCLUSION Maximin 2 show the dose dependent effect on cell growth suppression; The mechanism of antitumor of Maximin 2 seems not relate to the enhanced expression of HLA DR or HLA ABC, TNF ? and IFN ? also not found effect to HLA DR expression. Since IFN ? are able to increase the expression of HLA ABC expression,enhanced recognization and cytotoxity of CTL to bladder cancer cells. It is may be one of the mechanism of antitumor of IFN ?.
ABSTRACT
AIM: To study the boosting effect of Bombina maxima protein on bladder cancer cell lines growth and expression of telomerase. METHODS: Cell culture was used to dectect the effects of 3 blad der cancer cell lines growth; TRAP-PCR-ELISA method was used to detect the telomerase express under the ID50 culture concentration. RESULTS: Bombina maxima protein had the strong effect on growth of cell lines; particularly in the first 48 h; no effect was found in telomerase expression. CONCLUSION: Bombina maxima protein has the direct inhibitory effect on bladder cancer cell lines growth; The mechanism of antitumor of Bombina maxima protein is not by telomerase.