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1.
Chinese Pharmacological Bulletin ; (12): 1747-1752, 2022.
Article in Chinese | WPRIM | ID: wpr-1014242

ABSTRACT

To investigate the effect of Xinan Fang Yisheng-Qingluo-Huoxie prescription(YSF)on OPG/RANKL pathway in rats with rheumatoid arthritis induced by complete Freund's adjuvant(CFA)and its mechanism. Methods Thirty-two male SD rats were randomly divided into normal group, AIA group, QLY group and YSF group, with 8 rats in each group. AIA rats were induced except for the normal group. QLY and YSF were administered intragastrically on the first day after the model was successful for 30 days. The body weight and AI score of rats were observed. HE staining was used to observe the histomorphological changes of the ankle joint of rats. The expression of MMP3 and P-P65 was observed by immunohistochemistry. Serum levels of TNF-α, IL-1β, OPG and RANKL were detected by ELISA. The expression of OPG and RANKL proteins in synovium of rats was detected by Western blot and RT-PCR. Results Compared with AIA model, the body weight of the rats gradually returned to normal, and inflammation score decreased. HE staining showed that the bone was well preserved, no synovial infiltration was observed after YSF administration, and MMP3 and P-P65 showed low expression. The levels of TNF-α, IL-1β and RANKL in serum were significantly down-regulated, while the levels of OPG and OPG/RANKL were significantly up-regulated. In addition, Western blot and RT-PCR showed that OPG and OPG/RANKL were highly expressed in synovium of rats, while RANKL was on the contrary. Conclusions YSF may decrease inflammatory score and expression of TNF-α and IL-1β in serum by inhibiting inflammatory response. What's more, the increase of OPG/RANKL ratio can regulate the environment of joint bone destruction, thus alleviating RA.

2.
Chinese Traditional and Herbal Drugs ; (24): 1919-1925, 2016.
Article in Chinese | WPRIM | ID: wpr-853493

ABSTRACT

Objective: To study the therapeutic effect and mechanism of alcohol extract from Epimedii Herba (AEEH) on ovariectomy-induced osteoporosis in rats. Methods: Ipriflavone Tablets (200 mg/kg) and different doses of AEEH (170 and 85 mg/kg) were given for 8 weeks by ig administration, from 4 weeks after ovariectomization. Then, the protein expression of serum ALP, StrACP, femoral osteoprotegerin (OPG), and osteoclast differentiation factor (RANKL) was evaluated by biochemical assay kits and immunohistochemical evaluation; The mechanical properties of the tibia were determined by three point bending test method. Finally, the distal femoral trabecular bone parameters and the 3D reconstruction, trabecular bone of the 3D graphics were intuitively illustrated, and the effect of AEEH on rats suffered from osteoporosis role was observed by micro-CT. Results: Treatment with AEEH (170 mg/kg) significantly inhibited serum ALP and StrACP levels. Furthermore, AEEH also could significantly increase OPG protein expression while reduce RANKL protein expression compared with OVX rats. In addition, it could effectively improve the biomechanical properties of the tibia and the parameters of the trabecular bone of the femur, respectively. Conclusion: AEEH is effective in treating ovariectomy-induced osteoporosis in rats and the mechanism is mainly achieved by promoting the activity of osteoblast and inhibiting the activity of osteoclast.

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