ABSTRACT
Resumen Objetivo. Realizar la caracterización morfológica y la evaluación clínica de sustitutos óseos de origen porcino, como material para la regeneración y reconstrucción de tejido óseo afectado en lesiones óseas bimaxilares. Método. Se analizaron características morfológicas de los materiales para regeneración (dispositivos médicos implantables) Biomec Cx y Biomod, el primero es una membrana acelular de submucosa intestinal y el segundo es sustituto óseo particulado y desmineralizado, ambos de origen porcino, por medio de técnicas de coloración y microscopía, con el objetivo de ver celularidad, organización de las fibras colágenas y tamaños de las partículas. Adicionalmente, se realizó un estudio de tipo comparativo prospectivo, con una muestra intencional de 31 pacientes, en los que se hicieron un total de 83 implantes. Los procedimientos quirúrgicos efectuados fueron: implante pos exodoncia más injerto óseo, implante dental e injerto óseo en el mismo tiempo quirúrgico e injerto óseo con un tiempo de 4 meses de integración para la colocación de implantes dentales. Para la evaluación del efecto regenerativo en los diferentes procedimientos quirúrgicos se interpretaron controles clínicos y radiográficos en los que se miró el comportamiento de la cresta ósea marginal. También se realizaron análisis histológicos de biopsias de tejido óseo regenerado. Resultados. En la caracterización morfológica se encontró que los materiales para regeneración son acelulares. La matriz formada por las fibras de colágeno del sustituto Biomec CX presenta una organización y espacios intersticiales que permiten una buena migración celular. El producto Biomod mostró una densidad y tamaño de partícula adecuada para el proceso regenerativo. En la evaluación clínica, todas las diferencias (mesial y distal) de los meses evaluados en relación con la ganancia de hueso, son estadísticamente significativas; prueba t pareada, p < 0,01 y ANOVA. El aumento promedio en 4 meses fue 0,235mm por mesial (57,3%) y 0,237mm por distal (56,7%). Conclusiones. A partir de los resultados obtenidos tanto en las características morfológicas como en la evaluación clínica de los sustitutos Biomec CX y Biomod, se comprobó que son materiales aptos para su uso en procesos regenerativos en los que se presenten lesiones óseas bimaxilares.
Abstract Objective. To perform the morphological characterization and clinical evaluation of porcine bone substitutes as a material for the regeneration and reconstruction of affected bone tissue in bimaxillary bone lesions. Method. Morphological characteristics of Biomec Cx and Biomod (implantable medical devices) regeneration materials were analyzed, the first is an acellular membrane of the intestinal submucosa and the second is a particle and demineralized bone substitute, both of porcine origin, by techniques of coloration and microscope with the objective of search the cellularity, organization of collagen fibers and particle sizes. Then a prospective comparative study was conducted with an intentional sample of 31 patients in which a total of 83 implants were made. The surgical procedures were: Post-extraction implant plus bone graft, dental implant- bone graft at the same time and bone graft with a time of 4 months of integration for the placement of dental implants. For the evaluation of the regenerative effect in the different surgical procedure we interpreted clinical and radiographic controls with the behavior of the marginal bone crest. Histological analyzes of biopsies of regenerated bone were also performed. Results. In the morphological characterization it was found that the materials for regeneration are acellular. The matrix formed by the collagen fibers of the substitute Biomec CX presents an organization and interstitial spaces that allow a good cellular migration. The Biomod product showed a density and particle size suitable for the regenerative process. In the clinical evaluation, all the differences (mesial and distal) of the months evaluated in relation to the bone gain, are statistically significant; T-test, p <0.01 and ANOVA. The average increase in 4 months was 0.235mm per mesial (57.3%) and 0.237mm per distal (56.7%). Conclusions. Based on the results obtained both in the morphological characteristics and in the clinical evaluation of the substitutes Biomec CX and Biomod, it was verified that they are suitable materials for use in regenerative processes in which bimaxillary bone lesions are present.
Subject(s)
Humans , Osteology , Bone Regeneration , Bone Substitutes , Bankart LesionsABSTRACT
[Objective]To study the biocompatibility of nacre on cultured human osteoblasts in vitro.[Method]The iliac bone of human were added with collagenase-trypsin,a lot of osteoblasts were released out.Then the osteoblasts were purified and cultured.Subsequently,the 3rd generation cultured human osteoblasts were inoculated with the extract of three materials:nacre,hydroxyapatite,and rubber.The appearance of osteoblast was observed under the phase contrast microscope.Microstructure of the osteoblast was observed under transmission electron microscope.Additionally,to determine whether these three materials affect cell proliferation,the numbers of BrdU-labeled cells were examined at three groups.MTT assay was tested to observe the viability of human osteoblasts.[Result]Under the phase contrast microscope,the appearance of the osteoblasts in two groups were spindle or triangle like,and black nodes were seen in confluent cell layer 19 days after cultured.The observation of electron microscopy showed that the nucleus of the osteoblasts in two groups were big and round,and there were a large number of mitochondrion and rough endoplasmic reticulum in two groups.Stereological analyses of the numbers of BrdU-labeled cells revealed that females produced more cells than males in the dentate gyrus but not in the subventricular zone.[Conclusion]The nacre have no harmful effect to character of biology of human osteoblasts in vitro.
ABSTRACT
Periodontal regeneration therapy with bone-substituting materials has gained favorable clinical efficacy by enhancing osseous regeneration in periodontal bony defect. As bone- substituting materials, bone powder, calcium phosphate ceramic, modified forms of hydroxyapatite, and hard tissue replacement polymer have demonstrated their periodontal bony regenerative potency. Bone-substituting materials should fulfill several requirements such as biocompatibility, osteogenecity, malleability, biodegradability. The purpose of this study was to investigate biocompatibility, osteo-conduction capacity and biodegradability of Na2O, K2O added calcium metaphosphate(CMP). Beta CMP was obtained by thermal treatment of anhydrous Ca2(H2PO4)2. Na2O and K2O were added to CMP. The change of weight of pure CMP, Na2O-CMP, and K2O-CMP in Tris-buffer solution and simulated body fluid for 30 days was measured. Twenty four Newzealand white rabbits were used in negative control, positive control(Bio-Oss), pure CMP group, 5% Na2-CMP group, 10% Na2O-CMP goup, and 5% K2O-CMP group. In each group, graft materials were placed in right and left parietal bone defects(diameter 10mm) of rabbit. The animals were sacrificed at 3 months and 6 months after implantation of the graft materials. Degree of biodegradability of K2O or Na2O added CMP was greater than that of pure CMP in experimental condition. All experimental sites were healed with no clinical evidence of inflammatory response to all CMP implants. Histologic observations revealed that all CMP grafts were very biocompatible and osseous conductive, and that in K2O-CMP or Na2O-CMP implanted sites, there was biodegradable pattern, and that in site of new bone formation, there was no significant difference between all CMP group and DPBB(Bio-Oss) group. From this result, it was suggested that all experimental CMP group graft materials were able to use as an available bone substitution.