ABSTRACT
Objective To investigate the developmental characters of neural stem cells(NSCs) in occipital of cortex from human fetal brain at different age.Methods Ninety cases of embryoes at gestational age 16-32 weeks and by induction of labor with water bag were collected for determining distribution,shapes,growth modes and the number of NSCs in the occipital of cortex with immunohisto- chemical method under light microscope.Results It was noted that NSCs existed in the occipital of cortex from human fetal brain at different ages.NSCs mainly distributed in layers of cone cells and inner granule cells.NSCs existed in the occipital of cortex of different fetal age included middling round cells,NSCs had enations from 0 to 1.Nucli were larger than plasm.Each NSC had nucleoli from 2-4 and rarefaction chromatin.Most of NSCs distributed in three growth modes including crowd,cluster and clone,occasionally with a single growth mode among other nerve cells.There were no differences including distribution,shapes,growth modes and the number of NSCs in the occipital of cortex between groups,but,NSCs gradually decreased with increasing of age.Conclusion NSCs exists in the occipital of cortex from different gestational age,and the number of NSCs decreases with increasing of age.
ABSTRACT
Objective:To investigate the changing characteristics of neural stem cells(NSC) in the temporal lobe of cortex from human fetal brain at different age.Methods:Ninety embryos delivered by induction labor with water bag at gestational age 16-36 w were collected,distribution,Shapes,growth modes and the number of NSC in the temporal lobe of cortex were determined with immunohistochemical method under light microscope. Results:NSC existed in the temporal lobe of cortex from human fetal brain and were round,elliptic or polygonal in shape,especially small round,NSC distributed mainly in cone cell and inner granule cell layers.NSC had enations from 0 to 2,nucleoli from 1 to 2 and rarefactive chromatin,nucli were large.Most NSC distributed in clones with a few NSC existed in a single mode among other nerve cells.There were no differences between groups in the distribution,shapes,species and growth modes of NSC in the temporal lobe of cortex,but the numbers of NSC gradually decreased with increase of age.Conclusion:There were no differences on the distribution,shapes,species and growth modes of NSCs in the temporal lobe of cortex from different gestational age,the number of NSC decreased with increase of age.