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[Objective] To discuss the expression and meaning of NOS and BDNF in the lateral zone of rats’ hypothalamus with diabetes. [Method] Inject STZ 50mg/kg into the abdomen for rats model of diabetes, after 1 month, observe the positive neuron expression of LH NOS in rats’ hypothalamus with diabetes with NADPH-d enzyme histo-chemical method, to the positive neuron expression of LH BDNF with NADPH-d immunohisto-chemical method. [Result] The expression of NOS and BDNF is both lowered compared with normal group, P
ABSTRACT
Recently,achievement has been made in using brain neuron transplantation to treat central nerve damage and degradation diseases,which atract another wide concern from the medical circle.This article reviews the history,techniques and clinical application of brain neuron transplantation.
ABSTRACT
Objective To investigate the distribution of interleukin\|2 receptor (IL\|2R) and glucocorticoid receptor(GR) and identify coexistence of IL\|2R and GR in the rat brain. Methods The double labeling immunocytochemical technique(PAP method combined with ABC method), DAB and BDHC were used in the double labeling immunocytochemical method as the chromogens respectively. The reactive products of former was brown or yellow and later was black blue. Results IL\|2R and GR positive neurons were widely distributed in the cerebral cortex, hippocampus, thalamus, many motor and sense nuclei in the brain stem. The immunoreactive products of IL\|2R were found to be located on cell membrane and GR in nucleus and cytoplasm. There were a lot of positive double labeling neurons in the rat brain. The rate of double labeled cells in the total number of positive cells varied in different regions of brain, such as, 50 percent in cerebral cortex and 30 percent in nucleus of abducent nerve. Conclusion Immunogical cytokines and hormone could regulate the neuronal function through their corresponding receptors which coexisted in the same brain neurons. The present study might provide morphological evidence in the level of receptor for the immuno\|neuro\|endocrine network.