ABSTRACT
Objective To evaluate the early diagnosis of patients with T1 and Tis breast cancer,and to ana-lyze and explore the diagnostic efficacy of breast cancer.Methods 242 patients with T1 and Tis breast cancer who received treatment were selected.The diagnostic accuracy and detection rate of two kinds of imaging methods for breast cancer were compared.Results 35(13.46%),91(35.00%),109(41.92%) and 25(9.62%) of the dense breast were classified by the X-ray of the breast.In the detection rate, fat type and a small amount of fiber gland body breast,the detection rate of X ray was significantly higher than that of ultrasound examination,the differences were sta-tistically significant (χ2 =8.74,21.70,all P0.05).For the dense breast,the detection rate of ultrasonography was significantly higher than breast X-ray,and the difference was statistically significant (χ2 =6.64,P0.05).Conclusion For a small amount of T1 and Tis breast cancer patients,the early screening accuracy and detection rate of ultrasound examination is more suitable for the volume of T1 and Tis in patients with breast cancer.
ABSTRACT
Objective To investigate the proliferation, immune phenotype and cytotoxicity on different cell lines of cytokine-induced killer (CIK) cells collected from healthy donors. Methods Peripheral blood mononuclear cells (PBMC) from healthy donors were induced to become CIK cells by adding cytokines including rhIL-2, rhIFN-γand CD3 McAb. The proliferation of CIK cells was tested by blood cell recording board. The CIK cells were analyzed on different time points by FACS. The cytotoxicity of CIK cells against different tumor cell lines, such as K562, BJAB, A549, MCF-7 and HepG2, was detected by MTT assays on day 13. Results CIK cells quickly proliferated from day 5, and expanded by 182-fold after 20-day culture. The immunophenotypes of CD3+, CD3+CD8+and CD3+CD56+were (97.83±1.03)%, (77.12±1.60)%and (27.58± 2.02)%. The percentages of CD3+, CD3+CD8+and CD3+CD56+increased noticeably (P＜0.01). According to the effector-tar-get ratio of 40∶1, the activity of CIK cells against tumor cells K562, BJAB, A549, MCF-7 and HepG2 were (88.89±7.22)%, (75.42±9.52)%, (63.19±5.67)%, (43.53±5.67)%and (42.63±7.69)%. The experiments showed that CIK cells possessed high-er antitumor cytotoxic activity. Conclusion CIK cells can be largely capacity cultured by adding cytokines in vitro. CIK cells were a highly efficient cytotoxic cell against tumors, and had clinical application potentials.