ABSTRACT
Objective To explore the expression of c-met mRNA in nasopharyngeal carcinomas(NPC) and its relation with clinical biological behavior. Methods In situ hybridisation was used to detect mRNA expression of c-met in 15 cases NP and 55cases NPC. Results The positive rate of c-met mRNA in NP and NPC cells were were 13.3 %(2/15) and 61.8 %(34/55) respectively. The expression of c-met mRNA was significantly correlated with lymphnode metastasis, local invasion(basilar destroying) (P 0.05). Conclusions The abnormality expression of c-met gene expression was well correlated with the biological behavior of metastasis and invasion. The expression of c-met mRNA could serve as an important index to estimate the prognosis of NPC. c-met may be a new diagnostic/therapeutic targert of NPC.
ABSTRACT
Objective To study the significance of c-met mRNA in axillary drainage after operations for breast cancer. Methods RT-PCR assay was used to examine c-met mRNA in axillary drainage after operations in 52 cases of breast cancer. The relationships between the expression of c-met and the tumor size, metastatic lymph nodes, the expressions of estrogen receptor (ER), progesterone receptor (PR) and c-erbB-2 were analyzed, respectively. In addition, the effect of douching operative field with 5-FU and distilled water on the expression of c-met mRNA was also analyzed. Results ①The proto-oncogene c-met mRNA could be detected in axillary drainage after operations for breast cancer by RT-PCR, and its positive rate was higher than that in routine pathological detection for micrometastasis in the axillary lymph nodes (P
ABSTRACT
The capacity of the liver to regenerate after a reduction in liver mass induced by surgical removal is quite remarkable. The most widely studied example of this phenomenon is accompanied by many investigators but the exact mechanism is not clear. Over the past decade, it has been demonstrated that growth factors play a major role in liver regeneration. Several growth factors such as epidermal growth factor, transforming growth factor(TGF), acidic fibroblast growth factor and hepatocyte growth factor(HGF) are involved in liver regeneration. Recently, HGF has aroused increasing interest due to its powerful mitogenic effect and multiple functions on various cell types. The receptor for HGF has recently been characterized as the product of the protooncogene c-met. We have examined the changes of synthesis of DNA and the expression of HGF mRNA and c-met mRNA that be involved in liver regeneration, after partial hepatectomy in the rat. Also we have examined the expression of TGF- mRNA that might be involved in inhibition of epithelial cell regeneration and differentiation of hepatocyte. Proliferative index was determined to use in vivo thymidine uptake. Nothern blot analysis used to assay HGF mRNA an TGF- mRNA. RNase protection assay used to assay c-met mRNA. The thymidine uptake of the remnant liver was abruptly increased after 18 hours, was maintained 24 hours. The nothern blot analysis showed that the expression of HGF mRNA and TGF- mRNA increased continuously within 24 hours. The RNase protection assay showed that the expression of c-met mRNA decreased continuously during one day after partial hepatectomy. The data presented here suggests that HGF is involved in liver regeneration and continuous decrease of c-met mRNA is phenomenon of receptor down-regulation. The increase of TGF- mRNA, well known as inhibitor of cell regeneration, suggests that TGF- is control mechanism of liver regeneration.