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Objective To investigate the effects of Simo decoction on ghrelin and calcitonin gene-related peptide (CGRP) in diabetic rats.Methods A total of 48 SD rats were divided into healthy control group,diabetes group (DM),DM treated with low dose Simo decoction group (DMTL),DM treated with medium dose Simo decoction group (DM-TM),DM treated with high dose Simo decoction group (DM-TH) and DM prevented with medium dose Simo decoction group (DMPM) ; there were eight rats in each group.The rats of DM group and other intervention groups were intraperitoneally injected with 1％ streptozotoein (STZ) to establish the model.The rats of healthy control group were injected with solvent.At eight weeks after the model successfully established,the rats of each intervention group were gavaged with low dose of Simo decoction (0.15 ml · kg-1 · d-1 ),medium dose of Simo decoction (1.5 ml · kg-1 · d-1 ) and high dose of Simo decoction (3.0 ml · kg-1 ·d-1 ) for two weeks; the rats of control group and DM groups were gavaged with equal volume of 0.9％NaCl.The rats of DM-PM group were gavaged with Simo decoction at 1.5 ml · kg-1 · d-1 for 10 weeks after the model successfully established.Rats were sacrificed at the end of drug intervention and gastric antrum tissues were dissociated for detecting the expression of ghrelin and CGRP by real time polymerase chain reaction (PCR).The data were analyzed by one way ANOVA and t test.Results There was no significant difference in ghrelin expression at mRNA level in gastric antrum tissues between diabetic rats and control group.However after intervened by low dose and medium dose of Simo decoction,the ghrelin expression increased (1.45 ± 0.34,1.87 ± 0.68 compared with 0.87 ±0.28,Dunnett's T3 test,P＜0.05).There was no effects of high dose Simo decoction on the expression of ghrelin in diabetic rats.There was no significant difference in ghrelin between DM-TM and DM-PM.The expression of CGRP at mRNA level in gastric antrum tissues of diabetic rats was significantly higher than that of control group (4.61 ± 1.67 compared with 1.06 ± 0.40,t =5.843,P＜0.01).There was no effects of low dose Simo decoction on CGRP expression of diabetic rats.The expression of CGRP of diabetic rats was down-regulated by medium and high dose of Simo decoction intervention.There was no significant difference in CGRP expression between DM-TM and DM-PM.Conclusions Simo decoction can promote ghrelin expression in gastric antrum of diabetic rats.The expression of CGRP in gastric antrum of diabetic rats significantly increased,but Simo decoction may down-regulate the expression of CGRP in gastric antrum of diabetic rats.
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0.01),which were significantly different from group Ⅲ(P
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Objective: To observe the influences of Dengzhan Xixin injection(灯盏细辛注射液) on(vascular) endothelial function and neurological deficit extent in patients with acute cerebral infarction.(Methods): Eightyseven patients with mild to moderate acute cerebral infarction were randomly(divided) into Dengzhan Xixin injection treatment group(treatment group,42 cases) and control group(45 cases) by the random method of envelope and card.The patients in both groups were treated with the same basic therapy.The treatment group was treated with 40 ml of Dengzhan Xixin injection,and the control group was treated with 16 ml of salvia miltiorrhiza injection.The therapeutic course was 14 days in both groups and 2 courses were applied.The changes of circulating endothelial cell count(CEC),the levels of endothelin(ET) and(calcitonin) generelated peptide(CGRP) in plasma as well as neurological deficit were measured and(compared).Results: The plasma CEC,ET in treatment group((8.0?3.6)cells/0.9 ?l and(52.92?(4.52)ng/L)) were significantly lower than those in control group((10.2?3.3)cells/0.9 ?l and(60.12?(3.23)ng/L)),but the plasma CGRP was higher than that in the(control) group on 14 th days((65.23?(1.52)ng/L) vs.(60.12?2.14)ng/L).The differences were significant between the two groups(P