ABSTRACT
Objective To explore the affinify of combination of Helicobacter pylori (Hp) and its L-form with surfactant protein D(SP-D),and to find function of SP-D in Hp infection and immunity. Methods SP-D was extracted from the lung of rats by Tris-HCl-EDTA and maltose-agarose affinity column. The combination of Hp and SP-D was detected by slide and tube agglutination tests,and the agglutination inhibition with SP-D immune serum was carried out. The L-form of Hp was induced by Ceftriaxon sodrum and identified by PCR and sequencing of the 16S rDNA fragment.The combination of SP-D and the L-form was tested by slide agglutination test. Results Agglutination of Hp NCTC11637 and SP-D was observed on slide,but NCTC11639,SS1 and the L-form failed to agglutinate. Agglutination was found 45 minutes after reaction and was inhibited by SP-D immune sera. Conclusion The ability of combination with SP-D was different between strains of Hp.The combination could be weakened or vanished when Hp lost the cell wall.
ABSTRACT
L-forms of Vibrio cholerae, El Tor biotype were induced by penicillin under the condition of liquid and solid media, and observed morphologically. It showed that the typical L-form colonies like oil-fried egg (L-form), granular form (Gform) and fibrous form (F-form) were seen after 3-10-time inducements under the microscope. At the beginning, it was observed that the bodies of bacteria expanded, and then became short-rod, long-chain and finally fibre-like bodies.