ABSTRACT
AIM: To study the FT-IR fingerprinting characteristic of Huangjinju Powder Injection(HJJ)(Flos Trollii chinensis,Flos chrysanthemi indici,Flos Lonicerae Japonicae) and its effective constituents.METHODS: We adopt FT-IR and the second order derivative FT-IR,analysis by the related ratio,and then describe its mass characteristic by its crude drugs and the effective constituents. RESULTS: HJJ has fifteen peaks that are in turn,3735,2931,1664,1614,1450,1408,1360,1291,1191,1067,915,768,687,597 and 417 cm~(-1).Its special peak in the second order derivative is 1416 cm~(-1).The characteristics of the constituents of its crude drug that Flos Trollii Chinensis has 2926 and 1609 cm~(-1),Flos Chrysanthemi Indici has 1660 and 1609 cm~(-1) and Flos Lonicerae Japonicae has 767.The effective constituents that Baicalin owns 1664,1614,1450,1408,1067,915,768,687,597 and 417 cm~(-1),Orientin owns 2931,1614,1291 and 1191 cm~(-1),Vitexin owns 1614,1360,1067 and 597,Linarin owns 1450,1067 and 597 cm~(-1),Chlorogenic acid owns 1614,1291 and 1191 cm~(-1),Caffeic acid owns 1450 and 1360,and Hyperin owns 1360 and 1291. CONCLUSION: The effective constituents of FT-IR can be used in evaluating the mass of Huangjinju Powder Injection and reflect the key characteristic of its essence.It owns the obvious characteristics and specificity.