Oral sirolimus in the treatment of adult eruptive cherry angiomas

Introduction@#Cherry angiomas are a common type of acquired vascular proliferation of the skin which manifest as single or multiple bright red spots that usually appear on the trunk and arms. They are generally asymptomatic; patients may opt to remove the lesions for cosmetic reasons and prevention of bleeding. Conventionally, most cherry angiomas are treated with curettage, laser, and electrosurgery. Herein, we report a case of multiple cherry angiomas managed alternatively with oral sirolimus. @*Case@#A 47-year-old Filipino female presented with a 10-month history of gradually enlarging multiple bright-red papules and pedunculated nodules with a propensity to spontaneously bleed on gentle manipulation involving the scalp and forehead. Clinicopathological correlation suggests a diagnosis of eruptive cherry angiomas. The patient was started on oral sirolimus, a mammalian target of rapamycin (mTOR) inhibitor.@*Conclusion@#We present a case of a patient with eruptive cherry angiomas who experienced significant decrease in size and bleeding with treatment of oral sirolimus with minimal adverse effects. For patients with eruptive cherry angiomas, especially with contraindicated comorbidities, first-line therapeutic option treatments with oral sirolimus can be beneficial.

Clinicopathological analysis of four cases of intravascular large B-cell lymphoma / 中华皮肤科杂志

Objective:To investigate clinicopathological features of 4 cases of intravascular large B-cell lymphoma (IVLBCL) .Methods:Clinical and pathological data were collected from 4 patients with histopathologically confirmed IVLBCL in Department of Dermatology, Peking Union Medical College Hospital from January 2020 to November 2020, and retrospectively analyzed.Results:The 4 patients were aged 57 - 76 years, including 2 males and 2 females. Of the 4 patients, all had neurological symptoms, 3 had fever, 3 exhibited impaired exercise tolerance and suffocation, and 3 exhibited pitting edema of the body. Case 1 presented with a cherry hemangioma-like papule measuring 0.2 cm in diameter on the back, and case 2 with telangiectasia on the left breast and upper abdomen. Six skin samples were taken from the 4 patients for histopathological and immunohistochemical studies, and tumor cells were found in 1 cherry hemangioma-like lesion and 1 lesion of telangiectasia, as well as in 2 of 4 normal skin samples. Histopathological findings mainly were dilated dermal blood vessels filled with large atypical mononuclear cells, and the atypical mononuclear cells were positive for CD20 immunohistochemically.Conclusion:For those patients with suspected IVLBCL, hemangioma-like and telangiectasia lesions tend to show characteristic histopathological and immunohistochemical findings, and a biopsy of normal skin can facilitate early diagnosis of IVLBCL.

A case of COVID-19 with papulovesicular rash that progressed to retiform purpura, accompanied by cherry angiomas

ABSTRACT CONTEXT: Various skin manifestations have been reported in coronavirus disease. It may be difficult to determine the etiology of these lesions in view of the increased frequency of handwashing during the pandemic, along with occurrences of irritant contact dermatitis and allergic contact dermatitis due to disinfectant use; usage of herbal medicine and supplements to strengthen the immune system; and urticarial or maculopapular drug eruptions due to COVID-19 treatment. The variety of associated skin manifestations seen with COVID-19 makes it challenging to identify virus-specific skin manifestations. Petechiae, purpura, acrocyanosis and necrotic and non-necrotic purpura, which can be considered as manifestations of vascular involvement on the skin, have been reported. CASE REPORT: Here, we report a case of eruptive cherry angiomas, which was thought to have developed due to COVID-19, with a papulovesicular rash on distal extremities that progressed over time to reticular purpura. CONCLUSION: The case presented had a papulovesicular rash at the onset, which evolved to retiform purpura, and eruptive cherry angiomas were observed. It should be kept in mind that dermatological signs may vary in patients with COVID-19.

Central retinal arterial occlusion in a patient with pyoderma gangrenosum

A 74-year-old male presented to us with a history of vision loss for 36 hours in the right eye (RE). The RE had a visual acuity of hand movements. The fundus revealed a pale retina, cattle tracking in the retinal vessels, and a cherry-red spot at the macula. The patient was a known case of pyoderma gangrenosum (PG) and had received intravenous methylprednisolone and cyclophosphamide at the onset of visual symptoms. An emergency anterior chamber paracentesis was performed following unsuccessful attempts of ocular massage. The patient improved to 6/9 in the RE 4 months after paracentesis. The patient had an aggressive course of PG, for which he needed a combination of oral steroid, immunomodulator therapy and biologicals. An association between central retinal arterial occlusion and PG has not been reported before, according to the best of authors' knowledge.

Adenopexia nictitante como alternativa quirúrgica del ojo cereza en caninos / Nictitating adenopexy as a surgical alternative for cherry eye in canines / Adenopexia nictitante como alternativa cirúrgica do olho cereja em caninos

Resumen Para esta investigación se realizó un estudio descriptivo no probabilístico en caninos que llegaron a consulta a la Clínica Veterinaria de la Facultad de Medicina Veterinaria y Zootecnia, Universidad de Córdoba, Colombia. Se describe el seguimiento médico-quirúrgico de 36 pacientes con edades entre los 10 y los 18 meses, de las razas bulldog inglés, cocker spaniel, beagle y mestizos de cocker spaniel con prolapso unilateral o bilateral de la glándula nictitante. En la exploración, la constante clínica fue epífora, inflamación crónica de la conjuntiva palpebral, masa ovalada, lisa, de tamaño variable, de color rosáceo con mayor intensidad en la superficie más expuesta, masa que sobrepasaba el borde libre del párpado inferior y de localización en relación con el canto palpebral nasal. El objetivo del trabajo fue describir una nueva técnica quirúrgica utilizada para la solución de una serie de 51 casos en 36 pacientes con prolapso de glándula nictitante en caninos, y que permitió su reposicionamiento.

Abstract For this research, a descriptive, non-probabilistic study was conducted in canines that were brought for consultation to the Veterinary Clinic of the Faculty of Veterinary Medicine and Zootechnics, at the Universidad de Córdoba, Colombia. This paper describes the medical-surgical follow-up of 36 patients aged between 10 and 18 months, of English bulldog, Cocker Spaniel, Beagle, and Cocker Spaniel mixed breeds, with unilater al or bilateral prolapse of the nictitating gland. On examination, the clinical constant was epiphora, chronic inflammation of the palpebral conjunctiva, and a pinkish, oval, smooth mass, with variable size and with greater intensity on the most exposed surface, which surpassed the free edge of the lower eyelid, located in relation to the nasal palpebral commissure. This research aimed to describe a new surgical technique used to solve a series of 51 cases in 36 patients with prolapse of the nictitating gland in canines, which allowed its repositioning.

Resumo Para esta pesquisa se realizou um estudo descritivo não probabilístico em caninos que chegaram à consulta na Clínica Veterinária da Faculdade de Medicina Veterinária e Zootecnia, Universidade de Córdoba, Colômbia. Descreve-se o seguimento médico cirúrgico de 36 pacientes com idades entre os 10 e os 18 meses, das raças bulldog inglês, cocker spaniel, beagle e mestiços de cocker spaniel com prolapso unilateral o bilateral da glândula nictitante. Na exploração, a constante clínica foi epífora, inflamação crônica da conjuntiva palpebral, massa ovalada, lisa, de tamanho variável, de cor rosáceo com maior intensidade na superfície mais exposta, massa que ultrapassava a borda livre da pálpebra inferior e de localização em relação com o canto palpebral nasal. O objetivo do trabalho foi descrever uma nova técnica cirúrgica utilizada para a solução de uma série de 51 casos em 36 pacientes com prolapso de glândula nictitante em caninos, e que permitiu seu reposicionamento.

Influence of Drying Conditions on the Final Quality of Cherry and Grape Tomatoes

ABSTRACT The convective drying process of cherry and grape tomatoes for dried tomato production was studied taking into account operational and sensorial aspects. The tomatoes were physicochemical characterized and dried at three air temperatures in a drying chamber. Thus, it was possible to the determinate the physicochemical characteristics, drying kinetics, thickness shrinkage, effective moisture diffusivity and activation energy. The effects of tomato type (cherry and grape), air temperature (60°C and 80°C), and final moisture (25% and 35% w.b.) were sensory evaluated utilizing a factorial experiment. The drying kinetics demonstrated that the drying processes occurred preferably in the falling rate-drying periods. The grape tomato showed a faster drying process, which was attributed to its higher surface area and also its internal structure. The sensory evaluation demonstrated that the cherry tomato, dried at lower air temperatures, resulted in better sensorial characteristics and higher purchasing intention, whereas the final moisture had no effect.

Wudang cherry ameliorates urate underexcretion and renal dysfunction in hyperuricemic mice / 中国药理学与毒理学杂志

OBJECTIVE To investigate effects of Wudang cherry on urate excretion and renal function and examined whether renal organic ion transporters were involved in potassium oxonate-induced hyperuricemic mice. METHODS The model of hyperuricemic mice was induced by intraperitoneal injection of potassium oxonate (250 mg·kg- 1) for 7 d. Water extracts of Wudang cherry at 500 mg·kg- 1 were orally administered to hyperuricemic mice for 7 d, benzbromarone (20 mg·kg- 1) and allopurinol (20 mg · kg- 1) were given as positive controls, vehicle control group was given equal normal saline.Serum and urine levels of uric acid were measured in hyperuricemic and normal mice. Simultaneously, the mRNA and protein levels of mouse urate transporter 1 (mURAT1), glucose transporter 9 (mGLUT9), organic anion transporters (mOAT1 and mOAT3), ATP- binding cassette, subfamily G, membrane 2 (mABCG2) and organic cation/carnitine transporters (mOCT1, mOCT2, mOCTN1 and mOCTN2) in the kidney were analyzed by Western blot, RT-PCR, immunohistochemical and immuno?fluorescent assay, respectively. RESULTS Wudang cherry significantly reduced serum uric acid levels and increased urine uric acid levels in hyperuricemic mice. And it effectively reversed potassium oxonate-induced alterations in renal mURAT1, mGLUT9, mOAT1, mOAT3 and mABCG2 mRNA and protein levels, resulting in the enhancement of renal urate excretion in mice. Moreover, Wudang cherry increased renal mOCT1, mOCT2, mOCTN1 and mOCTN2 mRNA and protein levels, and improved renal impairment in this model. CONCLUSION Wudang cherry processes uricosuric and nephroprotective actions by regulating renal organic ion transporters in hyperuricemic mice.

Wudang cherry ameliorates urate underexcretion and renal dysfunction in hyperuricemic mice / 中国药理学与毒理学杂志

OBJECTIVE To investigate effects of Wudang cherry on urate excretion and renal function and examined whether renal organic ion transporters were involved in potassium oxonate-induced hyperuricemic mice. METHODS The model of hyperuricemic mice was induced by intraperitoneal injection of potassium oxonate (250 mg·kg- 1) for 7 d. Water extracts of Wudang cherry at 500 mg·kg- 1 were orally administered to hyperuricemic mice for 7 d, benzbromarone (20 mg·kg- 1) and allopurinol (20 mg · kg- 1) were given as positive controls, vehicle control group was given equal normal saline. Serum and urine levels of uric acid were measured in hyperuricemic and normal mice. Simultaneously, the mRNA and protein levels of mouse urate transporter 1 (mURAT1), glucose transporter 9 (mGLUT9), organic anion transporters (mOAT1 and mOAT3), ATP- binding cassette, subfamily G, membrane 2 (mABCG2) and organic cation/carnitine transporters (mOCT1, mOCT2, mOCTN1 and mOCTN2) in the kidney were analyzed by Western blot, RT-PCR, immunohistochemical and immuno?fluorescent assay, respectively. RESULTS Wudang cherry significantly reduced serum uric acid levels and increased urine uric acid levels in hyperuricemic mice. And it effectively reversed potassium oxonate-induced alterations in renal mURAT1, mGLUT9, mOAT1, mOAT3 and mABCG2 mRNA and protein levels, resulting in the enhancement of renal urate excretion in mice. Moreover, Wudang Cherry increased renal mOCT1, mOCT2, mOCTN1 and mOCTN2 mRNA and protein levels, and improved renal impairment in this model. CONCLUSION Wudang cherry processes uricosuric and nephroprotective actions by regulating renal organic ion transporters in hyperuricemic mice.

Eugenia uniflora Dentifrice for Treating Gingivitis in Children: Antibacterial Assay and Randomized Clinical Trial

Abstract School-age children are frequently at high risk for the onset of biofilm-dependent conditions, including dental caries and periodontal diseases. The objective of this study was to evaluate the clinical efficacy of a dentifrice containing Eugenia uniflora Linn. (Surinam cherry) extract versus a triclosan-based comparator in treating gingivitis in children aged 10-12 years. The in vitro antibacterial potential of the dentifrice was tested against oral pathogens (Streptococcus mutans, Streptococcus oralis and Lactobacillus casei). Then a phase-II clinical trial was conducted with 50 subjects aged 10-12 years, with clinical signs of gingivitis. The subjects were randomly assigned to the experimental group (n=25) and control group (n=25), in which participants used the experimental dentifrice and a triclosan-based fluoridated dentifrice (Colgate Total 12(r)), respectively. Clinical examinations assessed the presence of gingivitis (primary outcome) and biofilm accumulation (secondary outcome) using the Gingival-Bleeding Index (GBI) and Simplified Oral Hygiene Index (OHI-S), respectively, at baseline and after seven days of tooth brushing 3x/day. The data were analyzed using paired and unpaired t-test (GBI) and Wilcoxon and Mann-Whitney (OHI-S), with p≤0.05. The experimental dentifrice showed efficient antibacterial activity in vitro. In the clinical trial, a significant reduction in gingival bleeding was observed in both experimental and control groups (p<0.0001), with no statistical difference between them (p=0.178), although a small size effect was observed. Biofilm accumulation was only reduced in the control group (p=0.0039). In conclusion, E. uniflora dentifrice showed anti-gingivitis properties in children aged 10-12 years. Thus, it may be a potentially efficient and safe product to be used alternatively in preventive dental practice.

Resumo Crianças em idade escolar apresentam, com frequência, alto risco para o desenvolvimento de doenças biofilme-dependentes, incluindo cárie e doenças periodontais. Este estudo investigou a eficácia clínica de um dentifrício contendo o extrato de Eugenia uniflora Linn. (pitanga) comparado a um dentifrício com triclosan no combate à gengivite em crianças de 10 a 12 anos. Foi avaliado o potencial antibacteriano in vitro do dentifrício sobre microorganismos da cavidade oral (S. mutans S. oralis and L. casei) e realizado um estudo clínico fase II incluindo 50 sujeitos, com sinais clínicos de gengivite, divididos aleatoriamente em dois grupos: Experimental - 25 sujeitos usaram o dentifrício contendo extrato de pitanga; e Controle - 25 sujeitos que usaram um dentifrício fluoretado contendo triclosan (Colgate Total 12(r)). Na baseline e após sete dias consecutivos de escovação, foi realizado o exame clínico para diagnóstico de gengivite (desfecho primário) e acúmulo de biofilme (desfecho secundário), utilizando o Índice de Sangramento Gengival (ISG) e Índice de Higiene Oral Simplificado (IHO-S). Os dados foram analisados utilizando o teste t pareado e não pareado (ISG) e teste de Wilcoxon e Mann-Whitney (IHO-S), com nível de significância de p≤0,05. Observou-se uma efetiva atividade antibacteriana do dentifrício experimental. No estudo clínico, observou-se redução de sangramento gengival em ambos os grupos experimental e controle (p<0,0001), não havendo diferença entre eles (p 0,178), embora com uma pequena magnitude de efeito. Apenas o grupo controle reduziu significantemente o acúmulo de biofilme (p=0,0039). Concluiu-se que o dentifrício experimental de E. uniflora mostrou-se eficaz na redução de gengivite em crianças de 10 a 12 anos. Assim, este dentifrício apresenta potencial para ser utilizado de forma eficaz e segura em odontologia preventiva.

Viability of probiotic bacteria and some chemical and sensory characteristics in cornelian cherry juice during cold storage

Background: Increased popularity of vegetarianism, lactose intolerance, and the high cholesterol content in dairy products, are all factors that have recently increased the demand for nondairy probiotic products. The objective of this study is to evaluate the effect of refrigeration on the viability of probiotics and asses some of the chemical and sensory characteristics in cornelian cherry juice. Results: The Iranian native probiotic strain (L. casei T4) showed greater viability compared to industrial types (viable count of 8.67 log cfu/mL versus <6.0 log cfu/mL at d 28). However, this most tolerant Iranian strain, could not withstand the conditions of 'Natural juice' at pH 2.6 for more than 7 d. Following a pH adjusted treatment (to pH ~3.5), the viability of the strain was improved to 28 d with some evidence of increased growth of the probiotic. However, the level of antioxidant activity, anthocyanin and phenolic compounds, revealed a slight decrease during cold storage. The changes in the chemical profile of the sample containing L. casei T4 indicated fermentation activity during cold storage. Sensory evaluation results showed significant differences between samples containing L. casei TD4 and other samples in taste, odor and overall acceptance in a complimentary way. Conclusion: The results showed that low pH and presence of inhibitor phenolic compounds of cornelian cherry juice have negative effect on viability of probiotics, especially industrial strains during refrigerated storage.

Vitamin C and antioxidant capacity stability in cherry and romaine during storage at different temperatures / 한국영양학회지

PURPOSE: The aim of this work was to study the change in antioxidant activity depending on storage temperature and storage period in romaine and cherry. METHODS: The plant material was stored at 0.7 +/- 0.6degrees C, 3.5 +/- 2.8degrees C, and 4.7 +/- 1.4degrees C. Cherry and romaine were stored for a period of 9 days and 7 days, respectively. The cherry was taken from each group of samples at regular intervals of days and the romaine was taken from each group of samples at regular intervals of 2days. Vitamin C, total polyphenol, and total flavonoid stability and antioxidant capacity including DPPH, total antioxidant capacity (TAC) were measured. RESULTS: For cherry, the levels of TAC and flavonoid were higher at the 0.7 +/- 0.6degrees C condition than other conditions (p 4.7 +/- 1.4degrees C > 3.5 +/- 2.8degrees C. CONCLUSION: The results indicated that the narrow differences and fluctuation in temperature were associated with antioxidant capacity and it might enhance the nutritional shelf life of vegetables and fruits.

Enfermedad de Tay-Sachs / Tay-Sachs disease

La enfermedad de Tay-Sachs es un trastorno neurodegenerativo progresivo de herencia autosómica recesiva. Se debe a la deficiencia de la enzima β-hexosaminidasa A, que provoca una acumulación de gangliósidos GM2 en los lisosomas. Se incluye dentro de las esfingolipidosis. De las esfingolipidosis que presentan mancha rojo cereza en la mácula, la enfermedad de Tay-Sachs es la única en la que no se evidencia hepatoesplenomegalia. La variante más frecuente se inicia en la lactancia. Se presenta un lactante del sexo masculino al que se le realizó el diagnóstico de esta entidad a los 8 meses de edad. A partir de los 4 meses comenzó a presentar una reacción de sobresalto. A los 6 meses comenzó a perder habilidades previamente adquiridas y crisis epilépticas mioclónicas. Se constató una disminución de la actividad específica de la enzima hexosaminidasa A en leucocitos.

Tay-Sachs disease is a progressive autosomal recessive inherited neurodegenerative disorder caused by Beta-hexosaminidase A enzyme deficiency that in turn provokes GM2 ganglioside accumulation in the lysosomes. It is included in the sphyngolipidoses classification. Among the sphyngolipidoses that present with cherry-red spot in the macula, Tay-Sachs disease is the only one that does not show hepatosplenomegaly. The most frequent variant begins at the breast-feeding phase. This report presented a male nursling who was diagnosed with Tay-Sachs disease at the age of 8 months. At 4 months of age, he had begun getting some fright reactions. At 6 months-old, he began losing his previously acquired skills and suffering myoclonic seizures. The cause was the reduced specific activity of the hexosaminidase A enzyme in leukocytes.

Cutaneous Manifestations of Diabetes Mellitus: A Cross-Sectional Study in a Tertiary Care Hospital

Background diabetes mellitus (DM) is the most common endocrine disorder. Mucocutaneous manifestations of DM are many and vary from trivial to life-threatening and often herald the onset of diabetes. Aims To study the incidence of mucocutaneous manifestations in diabetes mellitus with respect to demographic data such as age, sex and duration of diabetes mellitus. Materials and methods This is a cross-sectional observational study of 200 diabetic patients with cutaneous manifestations attending skin and diabetic clinic OPD. Detailed history, clinical examination and relevant investigations were done to diagnose the mucocutaneous disorders and complications of diabetes. Results Demographic profile showed majority of cases belonging to 5th decade (33%) and 4th decade (27%) with male preponderance. Cutaneous infections were most common (62%) followed by dermatoses more commonly associated with diabetes (58%). Some patients had more than one cutaneous manifestation. Conclusion Proper skin care and long-term control of blood glucose may reduce the risk of complications and is immensely beneficial to patients in long run.

Estudo retrospectivo de 71 casos de protrusão da glândula da terceira pálpebra (2009-2013) / Retrospective study of 71 cases of prolapsed third eyelid gland (2009-2013) / Análisis retrospectivo de 71 casos de prolapsos de glándula del tercer párpado (2009-2013)

Objetivou-se com esse estudo avaliar a incidência de protrusão da glândula da terceira pálpebra e correlacionar as características de raça e sexo com a lateralidade da alteração. Para isso, foram analisadas 3.151 fichas clínicas atendidas setor de oftalmologia veterinária da Universidade Estadual de São Paulo (UNESP) no período de 2009 a 2013, avaliando-se dados de incidência e lateralidade da afecção, além de idade, sexo e raça dos animais acometidos. Realizou-se a análise estatística de frequência e calculou-se o coeficiente de correlação de Spearman, considerando o nível de significância de 5%. Foram diagnosticados, no total, 71 casos de protrusão da glândula da terceira pálpebra em cães, correspondendo a 2,43% (71/2.916) da casuística de atendimento oftalmológico nesta espécie, porém não foi observado nenhum caso em gatos. Obteve-se uma maior frequência (58%, 41/71) em cães com idade inferior a 12 meses, sendo as raças mais acometidas: Lhasa Apso (24%, 17/71), Sem Raça Definida (20%, 14/71) e Beagle (14%, 10/71). Em 72% (44/71) dos casos a afecção se manifestou unilateral e 55% (39/71) dos animais eram fêmeas. Não houve correlação entre o sexo e a raça com a lateralidade da protrusão (p > 0,05). Com base no levantamento de dados realizados nesta instituição, conclui-se que a incidência da afecção é baixa em cães e rara em gatos, além de que não há influência do sexo e da raça com relação ao lado acometido pela alteração..

This paper aims to analyze the incidence of prolapsed third eyelid gland and correlate the breed and gender characteristics to the laterality of the alteration. In order to do this, three thousand one hundred and fifty-one (3151) medical records were analyzed at the Department of veterinary ophthalmology from the State University of São Paulo (UNESP) from 2009 to 2013, considering data such as the incidence and laterality of the disease, as well as age, gender and breed of affected animals. A frequency statistical analysis was performed and the Spearman correlation coefficient was calculated by taking the significance level of 5%. The prolapse of the third eyelid gland was diagnosed in 71 cases in dogs, which corresponds to 2.43% (71/2916) of the cases cared for in the ophthalmological assistance in this specie; however, no cases were observed in cats. Dogs younger than 12 months of age presented a higher frequency, (58%, 41/71). The breeds most affected were Lhasa Apso (24%, 17/71); Mongrel (20%, 14/71); and Beagle (14%, 10/71). The prolapse was unilateral in 72% (44/71) of the cases, and 55% (39/71) of the animals were female. There was no significant relation of gender and breed with the laterality of the prolapsed gland (p > 0.05). Therefore, it can be concluded that the incidence of the alteration is low in dogs and rare in cats, as well as no influence being related to gender and breed to the side affected by the alteration...

El objetivo de esta investigación ha sido evaluar la incidencia del prolapso de la glándula del tercer parpado y correlacionar las características de raza y sexo con la lateralidad de la alteración. Para tanto fueron analizadas 3151 fichas clínicas atendidas en el sector de oftalmología veterinaria de la Universidade Estadual de São Paulo (UNESP) entre 2009 y 2013, considerando datos de incidencia y lateralidad de la afección, además de edad, sexo y raza de los animales enfermos. Se realizó el análisis estadístico de frecuencia y fue calculado el coeficiente de correlación de Spearman, considerando el nível de significancia de 5%. Se ha diagnosticado 71 casos de prolapso de la glándula del tercer parpado en caninos, correspondiendo a 2,43% (71/2916) de la casuística del atendimiento oftalmológico en esta especie, sin embargo no se ha observado ningún caso en gatos. Hubo una frecuencia más grande (58%, 41/71) en perros con edad inferior a 12 meses, teniendo en cuenta que las razas que más presentaron este problema fueron Lhasa Apso (24%, 17/71), Sin raza definida (20%, 14/71) y Beagle (14%, 10/71). En 72% (44/71) de los casos, la afección se manifestó unilateral y 55% (39/71) de los animales eran hembras. No hubo correlación significativa entre el sexo y la raza con la lateralidad del prolapso (p > 0,05). Con base en el alzamiento de datos realizados en esta institución, se ha concluido que la incidencia de la afección es baja en perros y rara en gatos, además de no haber influencia del sexo y de la raza con relación al lado tomado por la alteración...

IDENTIFICATION OF KEY MOLECULAR COMPONENTS OF THE RESISTANCE OF CHERRY TOMATO AGAINSTPhytophthora infestans / Identificación de los principales componentes moleculares de la resistencia de tomate cherry contra Phytophthora infestans

Cherry tomato Solanum lycopersicum var cerasiforme cv Matt's wild cherry is a very resistant cultivar to most Phytophthora infestans isolates. Two isolates were identified, US940480 and US970001 that cause an incompatible and a compatible interaction respectively. US970001 is one of the few isolates producing a compatible interaction with this cultivar. To identify genes with a differential gene expression between compatible and incompatible interactions, gene expression patterns were analyzed with tomato cDNA microarrays including 12,899 independent tomato cDNA clones at different time points after inoculation. A diverse set of statistical tools were used to identify key components of the plant response to the pathogen. Forty-three genes were up-regulated during the incompatible reaction at time point 36 hours, 15 globally at all time points and twelve were found both in globally and at 36 hours. Northern blots analysis was performed to confirm differential expression showed by microarray analysis and to study the differential expression of more plant resistance genes (PR) genes between compatible and incompatible interactions for this interaction.

El tomate cherry Solanum lycopersicum var cerasiforme cv Matt's es bastante resistente a la gran parte de aislamientos de Phytophthora infestans. Se han identificado dos aislamientos, US940480 y US970001 que causan interacción incompatible y compatible respectivamente. US970001 es uno de los pocos aislamientos causantes de interacción compatible con este cultivo. Con el fin de identificar genes con expresión diferencial en interacciones compatible e incompatible, analizamos DNA copia de 12899 clones independientes en tres tiempos posteriores a la inoculación del patógeno. Se aplicaron diversas herramientas estadísticas para identificar componentes moleculares claves de la respuesta de la planta al patógeno. Cuarenta y tres genes fueron detectados como activados durante la interacción incompatible a las 36 horas posinoculación, 15 genes se detectaron como activados globalmente tomando en conjunto los 3 tiempos analizados y 12 genes tanto globalmente como a las 36 horas. Análisis de Northern blot permitieron confirmar la expresión diferencial detectada con los análisis de microarreglos y estudiar la expresión diferencial de otros genes de resistencia en plantas (PR) en in teracciones compatible e incompatible en esta interacción.

Sulphur aplicado no cultivo de Lycopersicon esculentum Mill / Azufre aplicado en el cultivo de Lycopersicon esculentum Mill / Sulphur dilution applied on Lycopersicon esculentum Mill Cultivation

Este trabalho teve como objetivo analisar o efeito do Sulphur diluído adicionado ao solo para o cultivo de Lycopersicon esculentum Mill. O tratamento consistiu na utilização de seis diluições de Sulphur na dinamização centesimal: 0c (controle), 6c, 12c, 18c, 24c e 30c, sendo que para o controle foi usado somente água destilada. O experimento foi conduzido em vasos com plantas em ambiente aberto, com aplicação semanal de 200 mL de diferentes diluições a 0,1% de Sulphur em água destilada, conforme tratamento. Os parâmetros avaliados na planta foram o crescimento da parte aérea, o início da floração, o número e a massa fresca de frutos. Os resultados evidenciam que houve efeito (p?0,01) dos tratamentos sobre o L. esculentum no crescimento das partes aéreas, número e massa fresca de frutos. Concluiu-se que a aplicação de Sulphur na diluição de 24c aumenta a produtividade de frutos em um sistema de produção orgânica, sem o uso de defensivos agrícolas e fertilizantes.

This study aimed to analyze the effect of Sulphur applied on Lycopersicon esculentum Mill cultivation. The treatment consisted in the use of six dilutions in centesimal scales of Sulfur: 0c (control), 6c, 12c, 18c, 24c, and 30c. For the control group, distilled water was used. The experiment was conducted in pots in an open environment, where 200 mL of different dilutions of Sulfur in distilled water at 0.1% were applied according to each treatment. The average plant growth of aerial parts, the beginning of flowering, number and fresh mass of the fruits were the parameters evaluated. The results shows that the treatments were effective (p?0.01) on the growth of the aerial part of L. esculentum, as well as on the number and fresh mass of fruits. It could be concluded that the application of Sulphur at 24c dilution increases the fruit productivity of tomatoes in an organic production system without using pesticides or fertilizers.

Este estudio tuvo como objetivo analizar el efecto del Azufre diluido y adicionado al suelo para el cultivo de Lycopersicon esculentum Mill. El tratamiento consistió en la utilización de seis diluciones de Azufre en la potenciación centesimal de 0c (control), 6c, 12c, 18c, 24c y 30c, siendo que para el control se utilizó agua destilada. El experimento se llevó a cabo en macetas con plantas en ambiente abierto, con aplicación semanal de 200 mL de diferentes diluciones a 0.1% de Azufre en agua destilada, conforme tratamiento. Los parámetros evaluados en la planta fueron: el crecimiento de la parte aérea, el inicio de la floración, el número y la masa fresca de frutos. Los resultados evidencian que hubo efecto (p?0.01) de los tratamientos sobre L. esculentum en el desarrollo de las partes aéreas, número y masa fresca de frutos. Se concluyó que la aplicación de Azufre en la dilución de 24c aumenta la productividad de frutos en un sistema de producción orgánico, sin el uso de pesticidas y fertilizantes.

Niemann-Pick Disease Type A Presenting as Unilateral Tremors.

Niemann-Pick group of diseases are rare lysosomal storage disorders. The clinical phenotype is variable. We report a child who first time presented with tremors of tongue and tremors of one side of the body. On examination child had hemiparesis and hepatosplenomegaly. Bone marrow examination shows storage cells suggestive of Niemann-Pick cells and enzyme assay confirmed the diagnosis.

Avaliação de 67 casos de protrusão da glândula da terceira pálpebra em cães (2005-2010) / Evaluation of 67 cases of prolapse of the third eyelid gland in dogs (2005-2010)

Avaliaram-se 67 casos de protrusão da glândula da terceira pálpebra (cherry eye) em cães entre 2005 e 2010. Foram analisados a incidência da doença por gênero, a raça, a idade, o acometimento uni ou bilateral e a eficácia da técnica de Morgan pocket no reposicionamento da glândula. A protrusão da glândula da terceira pálpebra foi mais frequente em fêmeas (62,6%), e a raça mais acometida foi Lhasa Apso, seguida de cães sem raça definida (SRD). A idade dos animais variou entre dois meses e 13 anos (média de três anos), sendo mais frequente em animais com idade abaixo de 24 meses. A afecção manifestou-se no olho direito de 30 (44,8%) cães, no olho esquerdo de 18 (26,8%), e, em 19 animais (28,4%), a condição foi bilateral. Verificou-se que a técnica de Morgan pocket foi eficiente na resolução da afecção e requer mais cuidados no pós-operatório das raças grandes e gigantes.

In this study, 67 cases of dogs with prolapse of the third eyelid gland (cherry eye) between 2005 and 2010 were evaluated. The incidence of the disease by gender, age and breed was checked, and also if involvement was uni or bilateral and the effectiveness of the Morgan pocket technique of repositioning the gland. Prolapse of the third eyelid was more incident in females (62.6%), and Lhasa Apso and mongrel dogs were the most affected breeds. The age of the animals ranged between two months and 13 years (average of three years), and cases were more frequent in animals younger than 24 months. The disease manifested itself in the right eye of 30 (44.8%) dogs, in the left eye of 18 (26.8%) dogs, and in 19 animals (28.4%) the condition was bilateral. Morgan pocket technique was effective in the resolution of the complaint and demands more intensive care in post-surgery of large and giant breeds.

Complete Genome Sequence Analysis of Duck Circovirus Strains from Cherry Valley Duck / 中国病毒学·英文版

To investigate molecular epidemiology of DuCV in Cherry Valley ducks in China,the complete genomes of six DuCV strains,which were detected from Cherry Valley ducks in China between 2007 and 2008,were sequenced.Sequence and phylogenetic analysis were carried out to compare these six strains with another 27DuCV strains from Mulard duck,Muscovy duck,Pekin ducks and Mule duck.The analysis showed that the six DuCV strains exhibited typical genetic features of the family of DuCV,such as a stem-loop structure,three major open reading frames (Rep,Cap and ORF3),four intergenic repeats and the conserved motifs for rolling circle replication and for the dNTP binding domain located in the Rep protein.Phylogenetic analysis of the nucleotide sequences of the complete genome and Cap gene of these strains together with those that have been previously published demonstrated two distinct DuCV genotypes.The DuCV strains with complete genomes containing 1988and 1989 nucleotides clustered in genotype A,whereas the strains with complete genomes containing 1991,1992,1995 and 1996 nucleotides lay in genotype B.The six DuCV strains from Cherry Valley ducks were divided into the two groups.The results of the study provides some insight into the variation of DuCVs in Cherry Valley ducks.

Sulphur aplicado no cultivo de Lycopersicon esculenium mill / Suphur dilution applied on Lycopersicon esculenium mill cultivation / Azufre aplicado en el cultivo de Lycopersicon esculentum mill

Este trabalho teve como objetivo analisar o efeito do Sulphur diluído adicionado ao solo para o cultivo de Lycopersicon esculentum Mill. O tratamento consistiu na utilização de seis diluições de Sulphur na dinamização centesimal: 0c (controle), 6c, 12c, 18c, 24c e 30c, sendo que para o controle foi usado somente água destilada. O experimento foi conduzido em vasos com plantas em ambiente aberto, com aplicação semanal de 200 mL de diferentes diluições a 0,1% de Sulphur em água destilada, conforme tratamento. Os parâmetros avaliados na planta foram o crescimento da parte aérea, o início da floração, o número e a massa fresca de frutos. Os resultados evidenciam que houve efeito (p≤0,01) dos tratamentos sobre o L. esculentum no crescimento das partes aéreas, número e massa fresca de frutos. Concluiu-se que a aplicação de Sulphur na diluição de 24c aumenta a produtividade de frutos em um sistema de produção orgânica, sem o uso de defensivos agrícolas e fertilizantes.

This study aimed to analyze the effect of Sulphur applied on Lycopersicon esculentum Mill cultivation. The treatment consisted in the use of six dilutions in centesimal scales of Sulfur: 0c (control), 6c, 12c, 18c, 24c, and 30c. For the control group, distilled water was used. The experiment was conducted in pots in an open environment, where 200 mL of different dilutions of Sulfur in distilled water at 0.1% were applied according to each treatment. The average plant growth of aerial parts, the beginning of flowering, number and fresh mass of the fruits were the parameters evaluated. The results shows that the treatments were effective (p≤0.01) on the growth of the aerial part of L. esculentum, as well as on the number and fresh mass of fruits. It could be concluded that the application of Sulphur at 24c dilution increases the fruit productivity of tomatoes in an organic production system without using pesticides or fertilizers.

Este estudio tuvo como objetivo analizar el efecto del Azufre diluido y adicionado al suelo para el cultivo de Lycopersicon esculentum Mill. El tratamiento consistió en la utilización de seis diluciones de Azufre en la potenciación centesimal de 0c (control), 6c, 12c, 18c, 24c y 30c, siendo que para el control se utilizó agua destilada. El experimento se llevó a cabo en macetas con plantas en ambiente abierto, con aplicación semanal de 200 mL de diferentes diluciones a 0.1% de Azufre en agua destilada, conforme tratamiento. Los parámetros evaluados en la planta fueron: el crecimiento de la parte aérea, el inicio de la floración, el número y la masa fresca de frutos. Los resultados evidencian que hubo efecto (p≤0.01) de los tratamientos sobre L. esculentum en el desarrollo de las partes aéreas, número y masa fresca de frutos. Se concluyó que la aplicación de Azufre en la dilución de 24c aumenta la productividad de frutos en un sistema de producción orgánico, sin el uso de pesticidas y fertilizantes.