ABSTRACT
Aim To investigate the anti-angiogenic effect of 12a, the mastoparan-like peptide from wasp ( Vespa magnifica ) venom. Methods The tube for-mation and proliferation of human umbilical vein endo-thelial cells ( HUVEC ) and chicken chorioallantoic membrane ( CAM) model were used to observe the an-ti-angiogenic effect of 12a in vitro and in vivo, respec-tively. Results In the CAM model, 0. 1 μg or 1 μg of 12 a could markedly inhibit angiogenesis induced by 0. 2 μg rh-bFGF with heavy loss of color, decreasing density and obscure frame of the vessels. The inhibi-tion rates of angiogenesis were 60 . 2 % for 0 . 1 μg and 90. 3 % for 1 μg, respectively. Accordingly, in HU-VEC culture experiment, the proliferation and angio-genesis of HUVEC treated by 1 mg·L-1 and 10 mg· L-1 of 12 a were decreased by 55. 4 %, 39. 3 % and 51. 6 %, 26. 7 %, respectively. Conclusion 12a has a significant anti-angiogenic effect in a concentra-tion-dependent manner.
ABSTRACT
Angiogenesis is controlled by number of growth factors, including vascular endothelial growth factor (VEGF). Plant derived anti-angiogenic molecules acting via VEGF are being investigated for curtailing angiogenesis dependent diseases. In this study, methanolic (CM), n-hexane (CH), ethylacetate (CE) and water (CW) extracts of the roots of Calotropis procera were tested for anti-angiogenic activity. In the chicken egg chorioallantoic membrane (CAM) assay, CM, CH and CE but not CW inhibited VEGFinduced neovascularization in a dose-dependent manner. Of all the tested extracts, CM at the dose of 10, 5 and 2.5 ng most effectively inhibited over 83, 71 and 64%, of neovascularization induced by 10ng of VEGF, respectively. Sponge implantation assay in mice further showed that at the dose of 100ng CM, CH and CE but not CW significantly inhibited neovascularization induced by VEGF (100 ng). Taken together, this study indicates that the root extracts of C.procera may possess anti-angiogenic activity.
ABSTRACT
OBJECTIVE:To study the effect of eriocalyxin B (ER-B) on the proliferation of human umbilical vein endothelial cell (HUVEC) and hepatocelluar carcinoma angiogenesis. METHODS:The effects of ER-B at 6 kinds of concentration on the proliferation of HUVEC cultured in vitro were detected using modified MTT assay. Human hepatic cancer chicken chorioallantoic membrane angiogenesis model was induced to determine the inhibition effect of ER-B against angiogenesis. RESULTS:ER-B showed significant inhibitory effects on the proliferation of HUVEC in a dose and time dependent manner with IC50 about 7.27 ?g?mL-1. The moderate inhibition rate of ER-B in dose of 25 ?g?mL-1,50 ?g?mL-1 and 100 ?g?mL-1 to area of human hepatic cancer chicken chorioallantoic membrane angiogenesis were 14.15%,48.72% and 60.63%,respectively. 50 ?g?mL-1 ER-B has same effect to positive control. CONCLUSION:ER-B significantly inhibits the proliferation of HUVEC and chicken chorioallantoic membrane angiogenesis induced by human liver cancer cell.
ABSTRACT
Objective To compare with the different effect of the plasma and serum containing Danshen Injection (DI) on the angiogenesis. Methods The DI-containing plasma and serum at different concentrations and sampled at different time were prepared. The effect of DI-containing plasma and serum on the angiogenesis of chicken chorioallantoic membrane (CAM) was observed by using CAM model. Results The proliferation of vessels in all DI-containing plasms and serum groups and DI group was obvious as compared with that in the group of the blank plasma and serum(P