ABSTRACT
OBJECTIVE To study 39 kinds of resistant-related genes in a pan-resistant Alcaligenes xylosoxidans subsp xylosoxidans(AXXxx) in the sputum isolated from a severe hepatitis B patient.METHODS The susceptibility to antimicrobial agents were detected by MIC.16S rRNA and 39 resistant-related genes including 29 ?-lactamases genes,6 aminoglycoside-modifying enzymes(AMEs)genes,1 chlorhexidine-sulfadiazine resistant gene(qacE△1-sul1)and 3 intergron genes separated(intⅠ1,2,3) an AXXxx strain in the sputum of a severe hepatitis B patient were measured by PCR,and verified by DNA sequencing.RESULTS Among the strains,7 kinds of resistant-related genes(blaTEM-116,blaCARB-8,aac(6′)-Ⅱ,aac(3)-Ⅱ,ant(3″)-Ⅰ,qacE△1-sul1,and intI1)detected out.But other 27 kinds of ?-lactamases genes,3 kinds of AMEs(aac(6′)-Ⅰb,aac(3)-Ⅰ,ant(2″)-Ⅰ) genes,and 2 kinds of intⅠ(intⅠ2 and intⅠ3) genes were negative.CONCLUSIONS The pan-resistant A.xylosoxidans,mainly relates to 7 kinds of resistant-related genes(blaTEM-116,blaCARB-8,aac(6′)-Ⅱ,aac(3)-Ⅱ,ant(3″)-Ⅰ,qacE△1-sul1,and intⅠ1).
ABSTRACT
OBJECTIVE To study 40 kinds of resistant-related genes in a pan-resistant Pseudomonas aeruginosa.METHODS To detect the susceptibility of antimicrobial agents by MIC,40 resistant-related genes including 29 ?-lactamases genes,porin oprD2 genes,6 aminoglycoside-modifying enzymes(AMEs)genes,chlorhexidine/sulfadiazine resistant gene(qacE△1-sul1)and intergron(intⅠ1,2,3),etc,form 1 strain of P.aeruginosa were measured by PCR,and verified by DNA sequencing.RESULTS In the strain,there were positive of 6 kinds of resistant-related genes(blaTEM,blaOXA10,aac(6′)-Ⅱ,aac(3)-Ⅱ,qacE△1-sul1 and intⅠ1),but without oprD2 genes.Twenty-seven kinds of ?-lactamases genes,4 kinds of AMEs(aac(6′)-Ⅰb,aac(3)-Ⅰ,ant(3″)-Ⅰ and ant(2″)-Ⅰ),and 2 kinds of intⅠ(intⅠ2 and intⅠ3) were negative.CONCLUSIONS The multi-resistant mechanisms of pan-resistant P.aeruginosa are mainly related to 7 kinds of resistant-related genes(blaTEM,blaOXA10,oprD2,aac(6′)-Ⅱ,aac(3)-Ⅱ,qacE△1-sul1 and intⅠ1).
ABSTRACT
OBJECTIVE To study 39 kinds of resistant-related genes in a pan-resistant Burkholderia cenocepacia(BCE) strain,in the sputum from a severe hepatitis B patient.METHODS To detect the susceptibility to antimicrobial agents by MIC,16S rRNA,39 resistant-related genes including 29 ?-lactamases genes,6 aminoglycoside-modifying enzymes(AMEs) genes,chlorhexidine/sulfadiazine resistant gene(qacE△1-sul1),integron(intⅠ1,2,3),et al,of 1 strain of BCE in the sputum from a severe hepatitis B patient,were measured by PCR,and verified by DNA sequencing.RESULTS The strain was BCE conformed by 16S rRNA-PCR-DNA sequencing.It was susceptible to ceftazidime,cefepime,ciprofloxacin,levofloxacin,and trimethoprim/sulfamethoxazole,but resistant to piperacillin,aztreonam,cefotaxime,cefoxitin,meropenem,imipenem,nitrofurantoin,gentamicin and amikacin.There were positive of 6 kinds of resistant-related genes(blaTEM-116,aac(6′)-Ⅰb,aac(3)-Ⅰ,ant(2″)-Ⅰ,ant(3″)-Ⅰ,and intⅠ1),28 kinds of ?-lactamases genes,2 kinds of AMEs genes(aac(6′)-Ⅱ and aac(3)-Ⅱ),2 kinds genes of intⅠ(intⅠ2 and intⅠ3) were negative.CONCLUSIONS The multi-resistant BCE is with its multiple resistant mechanisms,and mainly relates to 6 kinds of resistant-related genes(blaTEM-116,aac(6′)-Ⅰb,aac(3)-Ⅰ,ant(2″)-Ⅰ,ant(3″)-Ⅰ and intⅠ1.