ABSTRACT
This study was aimed to establish a method for quality control of Tibetan medicine Shi-Wei Xiao-Shi-San (SWXSS). Thin layer chromatography (TLC) was established for the qualitative determination. Cinnamal and piperine were determined by HPLC. The separation was performed on Phenomenex C18 column (4.6 mm × 250 mm, 5μm). The results showed that TLC can be used in the identification of pomegranate seed and Terminalia chebula. The linear ranges of cinnamal and piperine were within the range of 0.06-0.37μg and 0.05-0.33μg, respectively. And the standard line was Y=9.273 2×106X-2.348 2×105, r=0.999 8;Y=7.315×103X-3.857, r=1.000 0. It was concluded that the identification method was specific, accurate and practical, which can be applied in the quality control of SWXSS.
ABSTRACT
AIM:To establish a method for determining menthol, methyl salicylate,cinnamal dehyde and engenol simultaneously in Baicao Oil by GC. METHODS: A separation was performed on a large bore capillary column(30m?0.53mm) with PEG-20M as the stationary phase of GC.The method was carried out with FID as the detector without split stream sampling.A programmed temperature was employed.Benzyl alcohol was selected as the internal standard substance. RESULTS: An excellent seperation of menthol,methyl salicylate,cinnamal and engenol in Baicao Oil was gained.The good linear relationships of menthol (62.7?10~3-2.01?10~3 ?g/mL),methyl salicylate (119?10~3-3.81?10~3 ?g/mL),cinnamal dehyde (0.42-205 ?g/mL),engenol (19.9-636 ?g/mL) were observed.All correlation coefficients were greater than (0.999 2).The average recovery of menthol,methyl salicylate,cinnamal and engenol were between(98.6%)～100.3%,and RSD was less than 2.4%. CONCLUSION: The method is simple,accurate and suitable for quality control of Baicao Oil.