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Objective To explore the roles of heme oxygenase-1 (HO-1 ) and protoporphyrin zinc IX (ZnPPIX ) , its inhibitor , in cisplatin chemotherapy for gastric cancer so as to provide potential targets for chemosensitivity in gastric cancer .Methods Gastric cancer cell line SGC7901 was used in vitro .MTT assay was carried out to determine the effects of ZnPPIX and CDDP on the proliferation in gastric cancer cells .The expression of HO-1 in gastric cancer cells was measured by Real-time PCR and Western blot ,respectively .The gastric cancer xenografts in nude mice were used to study the effects of ZnPPIX and CDDP in gastric cancer on tumor formation in vivo .Results The proliferation of cancer cells ,interfered by CDDP in combination with ZnPPIX ,could be significantly inhibited (P<0 .05) .Moreover ,CDDP could increase the expression of HO-1 in gastric cancer cells , which was reversed by ZnPPIX (P<0 .05) .The animal experiment showed that CDDP could inhibit gastric cancer growth in nude mice and reduce tumor volume and weight . Conclusion ZnPPIX could enhance the chemosensitivity of CDDP in gastric cancer ,which may be a potential sensitizer of cisplatin-based chemotherapy in gastric cancer .
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Objective To study the expression and significance of tumor metastasis suppressor gene-1(TMSG1) in esophageal squamous cell carcinoma (ESCC) and EC109 cells.Methods Immunohistochemistry S-P method was used to examine the expression of TMSG-1 protein in 136 cases of ESCC and 37 cases of normal esophageal mucosa.We analyzed the relationship between TMSG-1 and clinicopathological data of ESCC patients.EC109 cells were treated with 3 μg/mL of cisplatin (CDDP) in vitro for 24 h (the intervention group) and the control group was set up at the same time.The proliferation-inhibitory capability was analyzed with MTT assay.RT-PCR was used to examine the expression of TMSG-1 in the intervention group and the control group.Results The positive rate of TMSG-1 in ESCC and normal esophageal mucosa was 52.2％ (71/136) and 94.6％ (35/37),respectively.The expression of TMSG-1 in ESCC was significantly lower than that in normal esophageal mucosa (P＜0.05).The expression of TMSG-1 was related to TNM stage,differentiation degree and lymph node metastasis (P＜0.05).After EC 109 cells were treated with CDDP for 24 h,the proliferation inhibition rate was increased significantly compared with the control group (P＜0.01).RT-PCR results showed that the expression of TMSG-1 in the cells of the intervention group was significantly higher than that in the control group (P＜ 0.01).Conclusion The abnormal expression of TMSG-1 may play a role in the development and metastasis of ESCC.Examination of TMSG-1 may be useful for making diagnosis and guiding clinical therapy of ESCC.
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Objective To investigate the antagonist effects of sodium salicylate(NaSA) on the cisplatin(CDDP) induced hearing impairment in guinea pigs.Methods 60 guinea pigs were randomly divided into four groups: ①CDDP +NaSA(50 mg/kg) group,②CDDP +NaSA(100 mg/kg) group,③CDDP +NaSA(150 mg/kg) group and ④CDDP +NS(normal saline) control group.Auditory brainstem response(ABR) and distortion product otoacoustic emission(DPOAE) were used to evaluate their effects on hearing threshold and DPOAE amplitudes.Results The ABR responses of groupsⅠ,Ⅱ and Ⅲ were significantly lower than in the control group(P0.05).The ABR responses for group Ⅰ were significantly higher than group Ⅲ(P