ABSTRACT
Background: Inflammatory bowel diseases (IBDs) mainly consist of Crohn's Disease (CD) and Ulcerative Colitis (UC). These two categories have overlapping histopathological features and sometimes it is difficult to diagnose them into distinct category and such biopsies are categorised as Inflammatory Bowel Disease (IBD-U). Recently, there has been an increase in interest to discover new biomarkers of IBD to differentiate UC and CD and predict their prognosis. Method: In the present study, 273 non-neoplastic colonic biopsies with clinicoendoscopic features of IBD were studied and categorized into UC (88; 32.3%) and CD (03; 1.1%) but a major chunk remained in category of IBD-U (182; 66.6%). 161 (58.9%) of these biopsies were then subjected to IHC for RB protein and ?-catenin and Serology for pANCA and ASCA was done in only 85 (31.13%) of these selected cases for identification of UC and CD on colonic biopsies. Result: 161 biopsies that were subjected to IHC analysis included 57 cases of UC, 03 cases of CD, and rest 101 cases of IBD-U. Out of 101 cases of IBD-U, 87 (86.13%) cases were reclassified as UC (61; 60.3%) and CD (14; 13.86%) on the basis of results of IHC and Serology. Conclusion: The two major tools IHC for ?-catenin and RB protein and the assay of serum ASCA and p-ANCA along with proper history and clinical presentation can act as a good adjunct to conventional H and E in subclassifying cases of IBD-U into UC and CD.
ABSTRACT
Colorectal troubles are frequently common in medical practice ranging from mild nonspecific complaints to serious suffering. Colonic mucosal biopsies are considering one of the diagnostic tool in the evaluation of patients with colorectal pathologies. The objectives of this study are focusing for interpretation various spectrum of colonoscopic biopsies and to provide a guide to the plan of management strategy. This retrospective study was including 250 colonoscopic biopsies collected during the from December 2015 through January 2020. Among them 160 cases were of the Non-Inflammatory Bowel Disease Colitis (NIBDC) entities whereas, remaining 90 cases were Inflammatory Bowel Disease (IBD). Among the first one, 100 (40%) cases were Non-specific colitis, 13 (5.2%) bacterial colitis, 2 (0.8%) collagenous colitis, 15 (6%) hyperplastic polyp, 5 (2%) Peutz-Jeuger's polyps, 5 (2%) solitary rectal ulcer, 4 (1.6%) eosinophilic colitis, 3 (1.2%) Juvenile polyp, and 3 (1.2%) were melanosis coli, and remaining 10 (4%) cases were unremarkable. In regard to the IBD, 60 cases (24%) were ulcerative colitis and 30 (12%) Crohn disease. Majority of colonic troubles are linked to non-specific pathologies whereas, IBD is considering the second detectable colonic lesions in our study.