ABSTRACT
AIM: To improve on the acute cumulative death rates method in animal by intravenous (iv) administration. METHODS: A mathematic model was established to simulate the pharmacodynamic and pharmacokinetic process by the program written by Visual Basic.net. This program evaluated the acute cumulative death rates (ACD method) for use of pharmacokinetics of traditional Chinese medicine (TCM). RESULTS: The ACD method related on its first dosage, and an unsuitable dosage could lead to a wrong results. It also had been proved that, by adjusting the 2nd dosage it was a more suitable to wider range of dosage. CONCLUSION: By suitable 2nd dosage and enough animal number, ACD method can be used in the pharmacokinetics of TCM.
ABSTRACT
AIM: To improve the acute cumulative death rates method (ACD method) in animal by oral administration. METHODS: A mathematic model was established to evaluate the dose-toxic effect relationship by twice oral administration and the experimental steps were improved too. The methodological quality was explored using the simulation data from computer program and the real experimental date from the reference paper. RESULTS: The results showed that the experimental data could be fitted to its theoretical data from LD_5/2 to LD_ 95/2. Concentration-time curve after po ordinary powder of Semen Strychni in mice were fitted to a one-compartment with T_ 1/2(ka)= 1.136 h,T_ 1/2(ke)= 7.100 h,and T_ max= 3.576 h. CONCLUSION: The improved ACD method can be used in the pharmacokinetics of TCM by oral administration.