ABSTRACT
Objective To analyze the epidemiological characteristics of influenza in preschool children in Leshan City from 2016 to 2018, and to compare the difference between antigen colloidal gold reagent and nucleic acid detection reagent. Methods A total of 1 100 patients with suspected influenza admitted to Leshan City from January 2016 to December 2018 were selected as the research objects; the clinical data and characteristics of influenza epidemiology of the patients were collected, and the antigen colloidal gold reagent and nucleic acid detection reagent were used to detect influenza. Epidemiological characteristics; using nucleic acid detection as the standard to evaluate the diagnostic value of antigen colloidal gold reagent detection methods. Results The nucleic acid detection gold standard, antigen colloidal gold reagent method to detect influenza The sensitivity and specificity of the pathogen are both greater than 80%, and the AUC of influenza pathogen detection by the antigen colloidal gold reagent method is greater than 0.90, which has high application value . From 2016 to 2018, Leshan City was dominated by type B Y and type A H3N2; the main clinical features were cough, runny nose, and fever (P<0.05); the distribution of characteristics showed that there was no statistics on the sex of children with influenza Significantly, children less than 5 years old accounted for the highest proportion, and the proportion of children in winter and Spring Festival was significantly higher than that in summer and autumn, and the difference was statistically significant (P<0.05). Conclusion From 2016 to 2018, influenza virus infections in preschool children in Leshan City were mainly type Y and type A H3N2, and showed obvious age and seasonal characteristics. The antigenic colloidal gold reagent was used in clinical screening for children, but the test was negative. Children who are at high risk should be diagnosed with nucleic acid method as soon as possible.
ABSTRACT
Objective To compare the relationship between the enzyme‐linked immunosorbent assay(ELISA) reagent and West‐ern blot(WB) confirmation reagent for analyzing the quality lever of human T‐cell lymphotropic virus(HTLV) detection reagent . Methods The WB confirmation reagent was used to detect anti‐HTLV antibody in 156 human serum samples of ELISA prelimina‐ry screening positive .The ELISA cut‐off value(optimal value) was selected by using the two‐graph receiver operating characteristics (TG‐ROC) analytical method .The two‐by‐two table analysis was constructed to analyze the consistency of results detected by the two methods ,moreover the McNemar test was used to evaluate the consistency of detection results .The quality level of HTLV de‐tection reagent was comprehensively evaluated .Results Among 156 serum samples of ELISA preliminary screening positive ,only 40 samples were positive by the WB confirmation ,and other 116 samples were negative .The sensitivity and specificity of ELISA de‐tection reagent obtained by TG‐ROC analysis were 97 .5% and 45 .7% respectively ,the TG‐ROC test also indicated that the detec‐tion results had significant difference between ELISA and WB(P<0 .05) .By adjusting the cut‐off value ,the sensitivity and specific‐ity of ELISA were increased to 88 .8% (parametric method) .In the comparison of the parametric method and the non‐parametric method ,the obtained areas under the curve(AUC) was 0 .923 5(parametric method) ,their results were basically consistent .Conclu‐sion Although above results indicate that the detection results of ELISA reagent are different from those of WB ,but adjusting the cut off value can increase its sensitivity and specificity ,thus increases the reliability of diagnosis result .
ABSTRACT
In this assay, the reaction kinetics, optimum temperature, pH and various influential factors of ATP microbial rapid detection reagent by bioluminescence were studied. The results showed that it's enough for detection system to have 40 ~ 50?g/mL D-Luciferin. The light production decreased fastest in the first minute of reaction, then began to decay slowly. The optimal reaction temperature was 24℃~25℃and the optimal pH was pH 7.2 -7.4 in the reaction system. In addition, when stored at 4℃for 45h, the dissolved reagent solution could keep its 86% activity. When preserved at 25℃, the enzyme activity decreased less for 1h, and degraded gradually as time went by and only left 53. 5% of its activity after 6. 5h. While stored at 33℃, the enzyme activity decreased quickly with the time and only left 59. 1% after 1. 5h. The result indicated that storage temperature was a very important influential factor to the activity of reagent Meanwhile, different chemical substance such as acid, alkali, salt and surfactants inhibited the ATP bioluminescent reaction. When the concentration of NaCl reached 1. 5g/L, it could inhibit 52. 5% light production. Triton X-100, acid, and alkali also had some effects on the reaction, while CTAB, SDS and TCA would inhibit the bioluminescent reaction seriously.