ABSTRACT
This article was aimed to study the effect ofXiao-Qing-LongDecoction (XQLD) on plasma AngⅡ, ALD, Na+-k+-ATPase content, and plasma AT1 and AT2 mRNA expressions in Cor pulmonale rats, in order to further explore the mechanism of ventilating lung qi for diuresis. A total of 60 Wistar rats were randomly divided into the normal group, model group and XQLD, with 20 rats in each group. The enzyme-linked immunosorbent assay (ELISA) method was used to determine the AngⅡ, ALD and Na+-k+-ATPase content. The fluorescence quantitative PCR was used to detect the AT1 and AT2 mRNA expression. The results showed that compared with the normal group, the AngⅡ, ALD and Na+-k+-ATPase contents in the model group were significantly increased (P< 0.05, orP< 0.01). Compared with the model group, the AngⅡ, ALD and Na+-k+-ATPase contents in the XQLD group were obviously decreased (P< 0.05). Compared with the normal group, AT1 mRNA expression was increased; and AT2 mRNA expression was decreased in the model group (P<0.05, orP< 0.01). Compared with the model group, AT1 mRNA expression was decreased; and AT2 mRNA expression was increased in the XQLD group (P < 0.01). It was concluded that XQLD can effectively regulate the AT1 and AT2 mRNA expressions, influence ALD content to ventilate lungqi for dieresis.
ABSTRACT
Objective] To sum up director doctor Sheng Yuanliang ’s experience in treating severe hepatitis. [Method] Through analyzing the treating rules,causa morbi and mechanism of severe hepatitis,it sums up the key points of data of severe hepatitis,with 1 case for detailed explanation. [Result] The reason of the disease is mainly“damp,heat and toxin evils”,with corresponding therapies of removing heat to promote dieresis ,removing toxin and stasis. [Conclusion] The famous TCM doctor Shen Yuangliang’s applying clearing heat to promote dieresis,removing stasis and toxin to treat severe hepatitis,gets good clinical effect,having enlightenment meaning to clinical practice,with promotion value.
ABSTRACT
This study was aimed to investigate the anti-inflammatory, diuretic effect and acute toxicity of Herb of Lanceolate Sedge. The mice were randomly divided into 5 groups, which were the physiological saline group, aspirin group (0.05 g·kg-1), high dose (HD), middle dose (MD) and low dose (LD) of water extract of Herb of Lanceolate Sedge (10, 5, 2.5 g crude drug per kg) group. Intragastric administration of medication was given to mice once a day for 3 days. Auricular swelling induced by dimethyl benzene was used in the model establishment to calculate the swollen ear degree and the swollen inhibiting rate. And rats were randomly divided into 5 groups, which were the physiological saline group, aspirin group (0.05 g·kg-1), high dose (HD), middle dose (MD) and low dose (LD) of wa-ter extract of Herb of Lanceolate Sedge (10, 5, 2.5 g crude drug per kg) group. Intragastric administration of medi-cation was given to rats once a day for 5 days. Carrageenan induced paw edema was used in the model establishment to calculate the paw swollen rate. And rats were randomly divided into 5 groups, which were the physiological saline group, Furosemide tablets group (0.01 g·kg-1), high dose (HD), middle dose (MD) and low dose (LD) of water extract of Herb of Lanceolate Sedge (10, 5, 2.5 g crude drug per kg) group. Intragastric administration of medication was giv-en to rats once a day for 5 days. Water-loaded was used in the model establishment to calculate the 5-hour urine amount. The maximum tolerance dose (MTD) was used in the determination of the acute toxicity. The results showed that the three groups of HD, MD and LD of water extract of Herb of Lanceolate Sedge had obvious inhibiting effects on the swollen ear degree and the swollen inhibiting rate among mice, and the paw swollen rate of rats. It had signif-icant increasing effect on the 5-hour urine amount. The MTD of intragastric administration of water extract of Herb of Lanceolate Sedge was 640 g crude drug per kg for mice. It was concluded that Herb of Lanceolate Sedge dis-played obvious effects on anti-inflammatory and dieresis with low acute toxicity.