ABSTRACT
This study reports the action of digestive juice of snail Limicalaria flammea on raw starches. This juice exhibited maximum activity at pH 4.0-5.5 and was optimally active at 40-50 °C. About 82.5 % of the original amylasic activity still remained after treatment at 50 °C for 16 h. The digestive juice had a strong digesting ability towards various raw starches and efficiently hydrolyzed raw corn starch at a concentration of 1.0% and pH 5 at 40 °C in a period of 12 h. The rate of hydrolysis of the raw corn and cassava starches were 64.37 % and 42.0 % respectively while that of palmyrah palm was 29.05 %. The analysis of the hydrolyzed products of the raw starches by thin layer chromatography showed the glucose, maltosaccharide and dextrin after 12 h of hydrolysis.
ABSTRACT
Objective: To investigate the influence of the closed re-infusion of intestinal juice and EN on the treatment of intestinal fistula.Methods: The study group was composed of 21 patients of intestinal fistula from October 2004 to December 2006.The fistulae was in the 50 cm of the proximate jejunum and caused by trauma and operatin.The leak amount of intestinal juice was more than 1 000 mL.The protein level in serum and the absorption rates of the glucose,fat and nitrogen after the reinfusion of the intestinal juice were measured.Resutls: The protein level in serum and the absorption rates of the glusose,fat and nitrogen was increased after the re-infusion of the intestinal juice.Conclusion: The absorption of the glucose,fat and nitrogen in EN and the protein level in serum can be increased after the closed re-infusion of the intestinal juice.
ABSTRACT
Objective To eliminate the interference of CEA-related substances in CEA measurement and increase the specificity of CEA in the detection of malignant digestive diseases.Methods CEA level of peripheral blood and digestive juice(bile,gastric juice) from patients with benign or malignant digestive diseases was measured by ELISA,and semi-dry electrophoretic transfer method of Western blot technique to distinguish CEA and CEA-related substances.Results In malignant diseases,the CEA level of digestive juice was significantly higher than that in the blood,and there was no difference of CEA level in digestive juice and blood in benign diseases.Meanwhile,the CEA level of digestive juice and blood in malignant diseases were significantly higher than that in benign diseases.A specific band(molecular weight about 210?10~3) was detected in all malignant diseases except four cases whose CEA level was too low(less than 5 ?g/L),whereas no one of benign diseases had this specific band no matter how high or low the CEA level was.Conclusion The specificity of CEA detection in malignant digestive diseases can be improved by using digestive juice as sample and combining with Western blot technique.