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Objective:This study aims to analyze the threshold changes in distortion product otoacoustic emissions（DPOAE） and auditory brainstem response（ABR） in adult Otof-/- mice before and after gene therapy, evaluating its effectiveness and exploring methods for assessing hearing recovery post-treatment. Methods:At the age of 4 weeks, adult Otof-/- mice received an inner ear injection of a therapeutic agent containing intein-mediated recombination of the OTOF gene, delivered via dual AAV vectors through the round window membrane（RWM）. Immunofluorescence staining assessed the proportion of inner ear hair cells with restored otoferlin expression and the number of synapses.Statistical analysis was performed to compare the DPOAE and ABR thresholds before and after the treatment. Results:AAV-PHP. eB demonstrates high transduction efficiency in inner ear hair cells. The therapeutic regimen corrected hearing loss in adult Otof-/- mice without impacting auditory function in wild-type mice. The changes in DPOAE and ABR thresholds after gene therapy are significantly correlated at 16 kHz. Post-treatment,a slight increase in DPOAE was observeds,followed by a recovery trend at 2 months post-treatment. Conclusion:Gene therapy significantly restored hearing in adult Otof-/- mice, though the surgical delivery may cause transient hearing damage. Precise and gentle surgical techniques are essential to maximize gene therapy's efficacy.
Subject(s)
Mice , Animals , Otoacoustic Emissions, Spontaneous/physiology , Hearing/physiology , Ear, Inner , Hearing Loss/therapy , Genetic Therapy , Auditory Threshold/physiology , Evoked Potentials, Auditory, Brain Stem/physiology , Membrane ProteinsABSTRACT
@#Introduction: The prevalence of hearing loss ranges from 1 to 3 per 1000 live births and 2-4 per 100 high-risk babies as in low birth weight babies. Hearing screening in all newborns has not been fully implemented at Dr. Soetomo Hospital Surabaya so this research is expected to be the basis for hearing screening in all babies born especially low birth weight babies (LBW). The objective of the study was to predict the effect of low birth weight babies on impaired cochlear function in newborns. Methods: Cross-sectional comparative study was conducted from September 2018 to March 2019 in Dr. Soetomo Hospital. The subjects of this study were infants aged 2-28 days old in the nursery. Distortion Product Otoacoustic Emissions (DPOAE) was used to examineselected babies who fulfilled the selection criteria. Results: Twenty babies (≥2500 gram) and 20 babies (<2500 grams) were selected in this study. The proportion of cochlear dysfunction in infants with birth weights <1500 grams and 1500-2500 grams in Dr. Soetomo Hospital Surabaya was 50% and 35.7%. In multivariate analysis birth weight <1500 and birth weight 1500-2499 had a risk respectively 2.06 (95% Confidence Interval 0.08-48.26 P = 0.653) and 1.20 (95% Confidence Interval 0.07-20.34 P = 0.899) respectively. The proportion of cochlear dysfuntion in babies with birth weight ≥2500 grams is the highest which is 65 %. Conclusion: In this study Low Birth Weight Babies have a risk of 35.7 % of impaired cochlear function We would recommend DPOAE as screening method in all babies at Dr. Soetomo Hospital, Surabaya which is continued by second screening examination within one month on high risk babies and failed first test.
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Abstract Introduction: Previous research has suggested that individuals with different blood groups show varied incidences of noise-induced hearing loss. The reduced otoacoustic emissions amplitudes indicate the higher possibilities of outer hair cell damage for noise exposure. Objective: The objective is to analyze the characteristics of otoacoustic emissions, including the occurrence of spontaneous otoacoustic emission and the amplitudes of distortion product otoacoustic emission at certain frequencies in full term neonates with different ABO blood groups. Methods: A total of 80 selected full-term female neonates who passed the initial newborn hearing screen were enrolled into the study, with equal number of participants in four ABO blood groups (Blood Group A, Blood Group B, Blood Group AB, Blood Group O). Measurements of spontaneous otoacoustic emission and distortion product otoacoustic emission were performed in both ears for all participants. Results: (1) The blood group O participants showed significantly fewer spontaneous otoacoustic emission occurrences than the other three blood groups (A = 70%, B = 80%, AB = 67%, O = 25%, p < 0.05). (2) The blood group O participants showed lower DPOAE amplitudes at 1257 Hz (M = 4.55 dB, SD = 8.36), 1587 Hz (M = 11.60 dB, SD = 6.57), 3174 Hz (M = 7.25 dB, SD = 5.99), 5042 Hz (M = 13.60, SD = 6.70) than participants with the other three blood groups in left ears (p < 0.05). In right ears, the blood group O participants showed reduced amplitudes at 1257 Hz (M = 6.55 dB, SD = 8.36), 1587 Hz (M = 13.60 dB, SD = 6.57), 3174 Hz (M = 7.65 dB, SD = 6.43), 5042 Hz (M = 13.65 dB, SD = 6.50) than participants from non-O blood groups (p < 0.05). Conclusion: Female individuals with blood group O have lower otoacoustic emissions values than individuals with the other three blood groups. We need to further investigate the possible relationships between ABO blood group and cochlear function, including the potential influences of noise damage on cochlear outer hair cells.
Resumo Introdução: Pesquisas anteriores sugeriram que indivíduos de diferentes grupos sanguíneos apresentam incidências distintas de perda auditiva induzida por ruído. As amplitudes reduzidas das emissões otoacústicas indicaram maiores ou menores possibilidades de danos às células ciliadas por exposição a ruídos. Objetivo: Analisar as características das emissões otoacústicas, inclusive a ocorrência de emissões otoacústicas espontâneas e as amplitudes de emissões otoacústicas por produto de distorção em determinadas frequências em neonatos a termo de diferentes grupos sanguíneos do sistema ABO. Método: Foram incluídos 80 neonatos a termo selecionados na triagem auditiva neonatal inicial para participar do estudo, com número igual de participantes de grupos sanguíneos do sistema ABO (grupo sanguíneo A, grupo sanguíneo B, grupo sanguíneo AB e grupo sanguíneo O). As emissões otoacústicas espontâneas e emissões otoacústicas por produto de distorção foram medidas em ambas as orelhas de todos os participantes. Resultados: (1) Os participantes do grupo sanguíneo O apresentaram ocorrências de emissões otoacústicas espontâneas significantemente menores do que os dos outros três grupos sanguíneos (A = 70%, B = 80%, AB = 67%, O = 25%, p < 0,05). (2) Os participantes do grupo sanguíneo O apresentaram amplitudes de emissões otoacústicas por produto de distorção mais baixas a 1257 Hz (M = 4,55 dB, DP = 8,36), 1587 Hz (M = 11,60 dB, DP = 6,57), 3174 Hz (M = 7,25 dB, DP = 5,99), 5042 Hz (M = 13,0, DP = 6,70) do que os participantes dos outros três grupos sanguíneos nas orelhas esquerdas (p < 0,05). Nas orelhas direitas, os participantes do grupo sanguíneo O apresentaram amplitudes reduzidas em 1257 Hz (M = 6,55 dB, DP = 8,36), 1587 Hz (M = 13,60 dB, DP = 6,57), 3174 Hz (M = 7,65 dB, DP = 6,43), 5042 Hz (M = 13,65 dB, DP = 6,50) em comparação aos participantes de grupos sanguíneos não O (p < 0,05). Conclusão: Os indivíduos do sexo feminino do grupo sanguíneo O apresentaram valores menores de emissões otoacústicas do que os indivíduos dos outros três grupos sanguíneos. É necessário continuar a investigar as possíveis relações entre o grupo sanguíneo ABO e a função coclear, inclusive as possíveis influências do dano por ruídos às células ciliadas externas da cóclea.
Subject(s)
Humans , Female , Infant, Newborn , Otoacoustic Emissions, Spontaneous , Blood Group Antigens , Hair Cells, Auditory, Outer , Term Birth , Hearing Loss, Noise-Induced , NoiseABSTRACT
Objective To study and analyze the diagnostic value of auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE) in infants with secretory otitis media.Methods From June 2016 to June 2018,65 infants (120 ears) with secretory otitis media who were treated at the First People's Hospital of Taizhou were selected.All the 120 ears were diagnosed by ABR wave test and DPOAE test machine.The results of different diagnostic methods were observed and compared.Results Among 65 cases (120 ears),60 ears (50.00%) with mildly abnormality,43 ears (35.83%) with moderate abnormality and 17 ears (14.17%) with normal ABR wave Ⅴresponse threshold.There was no statistically significant difference in Ⅰ-Ⅴwave interval between the mild abnormal group and the normal group ( P ＞0.05 ), but Ⅰ -Ⅴ wave interval in the moderate abnormal group [( 4.27 ± 0.27)ms in moderate abnormal group ] was significantly shorter than that in the normal group [(4.75 ±0.31) ms] and the mild abnormal group [(4.73 ±0.21)ms],the differences were statistically significant (t＝5.949,9.722,all P＜0.05).TheⅤresponse threshold of bone -guided ABR wave was normal in 108 ears(90.00%,108／120).TheⅤlatency of bone guided wave was (8.16 ±0.22) ms,and abnormal in 12 ears (10.00%,12／120).With the increase of the gas conduction reaction threshold ,the bone conduction ABR reaction threshold also increased ,but it was not as obvious as the air conduction.The wave Ⅴ latency in the normal BRT group was significantly lower than that in the abnormal group ( compared with mild abnormal group , t ＝17.400,P ＜0.05;compared with moderate abnormal group,t＝130.015,P＜0.05).DPOAE test failed 86 ears (71.67%,86／120),passed through 34 ears (28.33%,34／120).Conclusion The latency of ABR wave I is sensitive to the diagnosis of secretory otitis media in infants.The combination of DPOAE and ABR is helpful to the early diagnosis of the disease.ABR and DPOAE tests have high value and are worthy of popularizing in clinic.
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Objective@#To study and analyze the diagnostic value of auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE) in infants with secretory otitis media.@*Methods@#From June 2016 to June 2018, 65 infants (120 ears) with secretory otitis media who were treated at the First People's Hospital of Taizhou were selected.All the 120 ears were diagnosed by ABR wave test and DPOAE test machine.The results of different diagnostic methods were observed and compared.@*Results@#Among 65 cases (120 ears), 60 ears (50.00%) with mildly abnormality, 43 ears (35.83%) with moderate abnormality and 17 ears (14.17%) with normal ABR wave Ⅴ response threshold.There was no statistically significant difference in Ⅰ-Ⅴ wave interval between the mild abnormal group and the normal group (P>0.05), but Ⅰ-Ⅴ wave interval in the moderate abnormal group[(4.27±0.27)ms in moderate abnormal group] was significantly shorter than that in the normal group[(4.75±0.31)ms] and the mild abnormal group[(4.73±0.21)ms], the differences were statistically significant(t=5.949, 9.722, all P<0.05). The Ⅴ response threshold of bone-guided ABR wave was normal in 108 ears(90.00%, 108/120). The Ⅴ latency of bone guided wave was (8.16±0.22)ms, and abnormal in 12 ears (10.00%, 12/120). With the increase of the gas conduction reaction threshold, the bone conduction ABR reaction threshold also increased, but it was not as obvious as the air conduction.The wave Ⅴ latency in the normal BRT group was significantly lower than that in the abnormal group (compared with mild abnormal group, t=17.400, P<0.05; compared with moderate abnormal group, t=130.015, P<0.05). DPOAE test failed 86 ears (71.67%, 86/120), passed through 34 ears (28.33%, 34/120).@*Conclusion@#The latency of ABR wave I is sensitive to the diagnosis of secretory otitis media in infants.The combination of DPOAE and ABR is helpful to the early diagnosis of the disease.ABR and DPOAE tests have high value and are worthy of popularizing in clinic.
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Background: Newborn hearing screening is conducted to identify suspected hearing loss and not to confirm the presence/absence of hearing loss or define features of the loss. Speech and hearing are interrelated, i.e., a problem with one could mean a problem with the other as speech and language is acquired normally through auditory system.Methods: A descriptive study conducted in the Department of Paediatrics, Dr. S. N. Medical College, Jodhpur, from June 2016 to December 2017. 5000 neonates were screened using otoacoustic emissions (OAE) in 2 stages at birth during 3rd to 7th day and 15-30 days respectively, followed by BERA at 3 months of age.Results: 1.4 infants per thousand infants had hearing loss. Presence of high-risk factors was seen to be associated be associated with hearing loss more than normal infants on screening with distortion product otoacoustic emissions (DPOAE) tests. However, on testing with BERA no such association was seen.Conclusions: 1.4 per 1000 infants had hearing loss. This study has shown that two stage distortion product otoacoustic emissions (DPOAE) hearing screening followed by british educational research association '(BERA) to confirm the hearing deficit, can be successfully implemented as new born hearing screening method in a hospital set-up, for early detection of hearing impaired, on a large scale, to achieve the high-quality standard of screening programs in a resource limited and developing nation like India.
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PURPOSE: Early detection and intervention of hearing impairment is believed to improve speech and language development and behavior of children. The aim of this preliminary study was to determine the prevalence of hearing impairments, and to identify the association of risk factors relating to refer response in high risk neonates who were screened using distortion product otoacoustic emissions (DPOAE). METHODS: The subjects included 871 neonates who were admitted to the neonatal intensive care unit of the Pediatric Department in Soonchunhyang University Bucheon Hospital from May, 2001 to December, 2004. They were screened using DPOAE. Based on DPOAE, we divided the neonates in two groups:'Pass' and 'Refer'. The differences in risk factors between the pass group and the refer group were analyzed. RESULTS: The incidence of the refer group was 12.1 percent(106 out of 871). The bilateral refer rate was 5.4 percent(47 out of 871). And the unilateral refer rate was 6.7 percent(59 out of 871). Gender, birth place, family history of hearing loss, small/large for gestational age, obstetrical factor, hyperbilirubinemia and use of gentamicin were not statistically related to the refer rate. Statistically related to refer rate were birth weight, resuscitated neonates, Apgar score, craniofacial anomaly, mechanical ventilator application, sepsis, using of vancomycin(P or = 60 dB) in this study was 2 percent(18 out of 871). CONCLUSION: This study showed a higher prevalence of hearing impairment in high-risk neonates. Thus neonatal hearing screening should be carried out in high-risk neonates.
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Child , Humans , Infant, Newborn , Apgar Score , Birth Weight , Gentamicins , Gestational Age , Hearing Loss , Hearing , Hyperbilirubinemia , Incidence , Intensive Care, Neonatal , Language Development , Mass Screening , Prevalence , Residence Characteristics , Risk Factors , Sepsis , Ventilators, MechanicalABSTRACT
BACKGROUND AND OBJECTIVES: The clinical utility of otoacoustic emissions (OAEs) has been well established in childhood hearing screenings. As middle ear negative pressure prevails in normal children, and since all OAEs must be transmitted through the middle ear before they can be measured in the ear canal, OAEs can be affected by middle ear negative pressure. The purpose of this study is to elucidate the effect of middle ear negative pressure on the detectability of OAEs. MATERIAL AND METHODS: TEOAEs were recorded for 36 ears using click before & after tonsillectomy and, or adenoidectomy (T and, or A) operations. Before & after T and or A operations, DPOAEs were generated at six geometric mean frequencies between 1-6 kHz for two different groups, Group I (40 ears) and Group II (36 ears). Group I: 3(f2/f1) ratios (1.21, 1.22 and 1.23) were used at the fixed stimulus level (70-70 dB SPL). Group II:3 stimulus levels were used (70-70 dB SPL, 60-70 dB SPL and 30-40 dB SPL). RESULTS: The negative middle ear pressure attenuated low frequency emission more than high frequency emission. CONCLUSION: Negative middle ear pressure may increase false alarm. So pneumatic otoscopy and aural immittance testing should be utilized to predict the feasibility of applying OAEs in childhood hearing screening test.
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Child , Humans , Adenoidectomy , Ear , Ear Canal , Ear, Middle , Hearing , Mass Screening , Otoscopy , TonsillectomyABSTRACT
Distortion product otoacoustic emissions(DPOAEs) have emerged as a promising clinical tool for measuring hearing status. This investigation examined the influence of four variable (test/retest reliability, test environment, gender and number of measurement points of resolution/octave) on the DPOAE response when obtained using a commercially available system, Otodynamics ILO92. Twenty normal hearing adults(10 males and 10 females) served as participants in this study. DPOAE measurements were obtained over three test sessions(baseline, 1 week retest, and a 10 minute retest following the one week measurement). Results showed that: 1) correlations were high across the three test sessions;2) test environment considerably affected the DPOAE measures below 4kHz;3) DPOAE amplitudes were significantly greater in females than males at 3 and 6kHz;and 4) DPOAE amplitudes were not affected significantly by the number of resolution points employed.
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Female , Humans , Male , HearingABSTRACT
BACKGROUND: Distortion product otoacoustic emissions(DPOAE) are otoacoustic emissions evoked by two pure tones. They are proposed as a frequency specific test of the mechanical properties of the cochlea. OBJECTIVE: The purpose of the this study were to investigate of basic properties of DPOAE in normal hearing subjects and correlations between DPOAE amplitudes and conventional pure tone auditory thresholds in patients with sensorineural hearing losses. MATERIAL AND METHODS: The amplitude and growth functions of distortion product otoacoustic emissions(DPOAE) at 2f1-f2, elicited by two primary tones f1 and f2 with a constant frequency ratio f2/f1=1.22 and varing geometric mean values 0.5, 1, 2, 4, and 8kHz, were measured for 20 normal subjects and 50 patients with sensorineural hearing losses. RESULTS: In normal hearing subjects 1) The average DP audiogram peaked at two frequency ranges, 1 kHz and 8 kHz. 2) In DPOAE amplitudes, inter-subject variability was relatively high, but inter-aural dispersion were remarkably small(correlation coefficent=0.72). In patients with sensorineural hearing loss 3) DPOAE amplitudes were correlated significantly with pure tone auditory thresholds in that higher auditoty thresholds showd lower amplitudes(correlation coefficenct=-0.65 at 1kHz 70dB SPL stimulus, -0.66 at 4kHz 70dB SPL stimulus). 4) Based on the presence or absence of DPOAE, the screening limit values of 50dB HL showed that sensitivity and specificity were 96%, 75% at 1kHz, 97%, 81% at 4kHz with 70 dB SPL stimulus.
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Humans , Auditory Threshold , Cochlea , Ear , Hearing , Hearing Loss, Sensorineural , Mass Screening , Sensitivity and SpecificityABSTRACT
Objective To assess the dynamic age-related hearing loss of inbred mouse C57 and BALB/c strains via distortion product otoacoustic emissions(DPOAE). Methods DPOAE levels were evaluated in C57 and BALB/c of 20,30,60,90,120,150,180 days old respectively,with the conditions that two pure tones,which stimulated the DPOAE,were at frequencies(f 1,f 2,f 1/f 2=1.22)and levels(L 1=70 dB SPL,L 2=65 dB SPL).Results DPOAE levels of C57 and BALB/c gradually decreased with age.From 60 days old,DPOAE levels of the two strains were dramatically decreased at 4.0,6.0,8.0 kHz( P
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Objective To investigate the usage of distortion product otoacoustic emissions(DPOAE) in normal infants hearing screening.Methods One hundred infants(200 ears) without clinical condition of illness were checked in GSI 70 DPOAE screening.The pure tones were 2,3,4 kHz,the OAE screener scored the test result as a pass,when the responses were above the PASS/REFER line and the signal-to-noise was at least 10 dB.Results Ninty-four infants(188 ears) passed hearing screening. Four infants(8 ears) with ceruminous impaction didn′t pass screening;after extracting impacted cerumen from the external auditory meatus,the 4 infants(8 ears) passed screening.Two infants(2 ears) with secretory otitis media didn′t pass screening,but after medicine treatment for 1 week,they passed screening too.Conclusion DPOAE has advantages in infants hearing screening.J Appl Clin Pediatr,2006,21(3):168-169