ABSTRACT
Objective To investigate the epidemiological characteristics of respiratory syncytial virus (RSV) positive children in Jiangsu, and to provide reference for the prevention and treatment of RSV infection. Methods A total of 11 574 children with respiratory tract infection from June 2018 to June 2020 were selected. RSV antigen was detected by direct immunofluorescence assay in all children. Other 6 kinds of respiratory viruses were detected in RSV positive specimens to understand the mixed infection. Results The positive rate of RSV in 11 574 samples was 12.34%(1 428/11 574), and the male to female ratio of RSV positive children was 1.92:1 (915/476). The detection rate of male children (12.79%) was significantly higher than that of female children (10.77%)(χ2=10.951, P2=27.48, P0.05). The positive rate of RSV from November to March was significantly higher than that from other months (χ2=9.451, P<0.05). The highest positive rate was 2 months (16.99%). Of the 1428 RSV-positive cases, 148 were co-infected with at least one other respiratory virus, accounting for 10.36%(148/1 428) of the RSV-positive cases. Among them, 117 cases were double infection and 31 cases were triple infection. RSV combined with PIV3 infection was the most common in 39 cases (26.35%). Conclusion RSV positive children are mainly concentrated in infants under 12 months of age in Jiangsu province, and the incidence is high from November to March. The protection of infants under 12 months of age should be strengthened, especially in hospitalized cases and male cases, which are often mixed with infection..
ABSTRACT
Objective To analyze the drug resistance status of mycobacterium tuberculosis in patients with double immunization of human immunodeficiency virus (HIV) and tuberculosis (TB) by phage bioassay (PhaB),and to optimize the control strategy.Methods One hundred and twelve cases of HIV/TB infected patients.in Chongqing Ninth People's Hospital were treated with PhaB method,and the drug susceptibility testing results were compared with 208 cases of simple pulmonary tuberculosis patients.Results The anti-tuberculosis drug resistance rate of HIV/TB patients was lower than that of simple pulmonary tuberculosis patients.The resistance rates of 5 common anti-tuberculosis drugs in HIV/TB patients were 7.14％ of isoniazid (INH),7.14％ of pyrazinamide (PZA),5.36 ％ of rifampicin(RFP) streptomycin(SM),and 4.46 ％ of ethambutol (EMB),compared with simple pulmonary tuberculosis(resistance rates of RFP were 17.31％,IN H 13.46 ％,PZA 11.54 ％,EMB 10.58 ％,SM 9.62 ％),RFP resistance rate of HIV/TB infected patients was lower(P＜0.05).There was no significant difference between two groups in the other four anti-tuberculosis drug(P＞0.05).The coincidence rate with the absolute concentration method were INH 96.4％,RFP 98.2％,PZA 96.4％,EMB 93.8％ and SM 96.4％,respectively.Conclusion The resistance rate of mycobacterium tuberculosis to RFP in patients with HIV/TB infection in this region is lower than that in patients with common pulmonary tuberculosis,which is related to the good medication compliance of these patients.PhaB has the characteristic of fast,simple,without special equipment,it can be used as a rapid screening of mycobacterium tuberculosis drug resistance method.