ABSTRACT
【Objective】 To evaluate the application of monoclonal typing reagents and human anti-A/B antibodies for absorption-elution test in ABO grouping. 【Methods】 The specificity of monoclonal typing reagents and human anti-A/B antibodies with standard A, B, O and AB phenotypes at 4 ℃, room temperature, and 37 ℃ were compared. Affinity was evaluated by the titer, agglutination time and agglutination intensity of the reaction with A1/B cells. 29 samples with ABO discrepancy were tested to evaluate the ability of monoclonal typing reagents and human anti-A/B antibodies to detect weak antigens in absorption-elution test. 【Results】 The specificity and affinity of human anti-A/B antibodies are low, and monoclonal typing reagents have cross reactivity. Human anti-A/B antibodies can detect most weak antigens in absorption-elution test with no cross reactivity. 【Conclusion】 In ABO grouping, the human anti A/B antibody binding absorption-elution test can serve as a supplement method for identifying ABO weak antigens. Accurate results can be obtained with reasonable reagents and corresponding methodology in serological tests,thus ensuring the safety of blood transfusion.
ABSTRACT
【Objective】 To evaluate the performance of absorption and elution test and real time PCR in identifying ABO blood group. 【Methods】 The blood samples with weakened antibody expression serologically were screened and analyzed by absorption and elution test, real time PCR and gene sequencing. The gene sequencing results were used as the gold standard to assess the consistency of the other two methods, and the sensitivity and specificity were compared. 【Results】 Compared with the sequencing results (gold standard), absorption and elution test showed moderate consistency, while real time PCR showed good consistency. Both the sensitivity and specificity of absorption and elution test and real time PCR were statistically different. 【Conclusion】 There was a good agreement between real time PCR and gene sequencing in identifying suspicious ABO blood group with weakened antibody expression serologically. Real time PCR, with a low misjudgment rate, has strong ability to detect weak antigen and is more conducive to identify blood type compared with absorption and elution test.
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【Objective】 To study the effect of intravenous immunoglobulin(IVIG) on the detection of blood transfusion compatibility in patients. 【Methods】 56 patients, submitted to our Hospital from March 1, 2017 to December 31, 2020, were enrolled as the research objects. They had negative unexpected antibody screening, major crossmatch incompatibility with the same blood type donors, and had a history of IVIG infusion. ABO and RhD blood groups typing, unexpected antibodies screening, crossmatch, direct antiglobulin test, indirect antiglobulin test, and acid elution test were all conducted by microcolumn gel method. 【Results】 After IVIG infusion, the initially major crossmatch incompatibility with the same blood type donors turned into compatiblity with O-type donors. Among them, 2 patients had transient discrepancy in ABO forward and reverse blood typing due to the IVIG infusion. IgG anti-A were detected in the red blood cell elution of 37 A-type patients; IgG anti-B in 2 B-type patients; 3 cases of IgG anti-A+ anti-B and 14 cases of solo IgG anti-A in 17 AB-type patients. 3 batches of IVIG preparations were detected randomly, IgG anti-A titer was 32-64, and IgG anti-B titer was 8-16. 【Conclusion】 The discrepancy in ABO forward and reverse blood typing and major crossmatch incompatibility with the same blood type donors may occur after non-O type patients received IVIG, which contains IgG types of anti-A and anti-B. In this situation, it is recommended to prepare major crossmatched O-type washed red blood cells to ensure the safety and effectiveness of clinical blood transfusion.
ABSTRACT
【Objective】 To identify one blood sample with ambiguous antibody screening result, explore the identification strategy of complex antibodies and provide blood transfusion plan. 【Methods】 Blood typing and antibody screening were performed by serological test. The genotyping of Diego blood group was carried out by gene detection. Absorption and elution test were designed to identify the complex antibodies. 【Results】 Serological test showed that the patient′s blood type was B, ccDEE, Jk(a-b+ ), Le(a-b+ ), Fy(a+ b-), Di(a+ b-). The results of genotyping showed that the Diego genotype of the patient was Di (a+ b-). Based on the above results, absorption and elution tests were designed and IgG anti-Dib, IgG anti-Ce, and IgG anti-Jka antibodies were detected in serum of the patient. Combined with the use of immunoglobulin and targeted blood transfusion measures, successful treatment was provided for the patient. 【Conclusion】 For the identification of antibodies against high frequency antigen combined with multiple antibodies, a variety of experimental techniques and methods should be combined, and the experimental scheme should be designed flexibly.
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BACKGROUND: Hemolytic disease of the fetus and newborn (HDFN) is a condition in which immune hemolytic anemia occurs in fetuses or newborns as a result of maternal alloimunized antibodies transfer. Antibody elution test and direct antiglobulin test (DAT) can be performed to diagnose HDFN; maternal originated antibodies cannot be confirmed if DAT is utilized alone. In this study, we analyzed the clinical significance of implementing concurrent DAT and antibody elution test in diagnosing HDFN. METHODS: We retrospectively analyzed the DATs and antibody elution tests that were simultaneously conducted in a period of 11 years, between 2005 and 2015, in newborns that received hemoglobin, reticulocyte, and total bilirubin tests. According to the results of these tests, the number of newborns diagnosed with HDFN was measured. Furthermore, the sensitivity and specificity of DAT and antibody elution test were compared. RESULTS: Among 325 newborns, the results of DATs and antibody elution tests were both negative in 208 (64.0%), negative and positive, respectively, in 80 (24.6%), positive and negative in 10 (3.1%), both positive in 27 (8.3%). When this was compared to the clinical diagnosis of HDFN, more sensitive and specific diagnoses were possible when implementing DAT and antibody elution test together (sensitivity of 76.9% for antibody elution test and specificity of 90.3% for DAT). Twenty-six (8.0%) newborns suspected for HDFN showed clinically significant hemolytic anemia. CONCLUSION: It is necessary to conduct both DAT and antibody elution test when HDFN is suspected. The severity of hemolysis in HDFN can be indirectly anticipated using an antibody elution test confirming maternal originated alloantibodies.
Subject(s)
Humans , Infant, Newborn , Anemia, Hemolytic , Antibodies , Bilirubin , Coombs Test , Diagnosis , Fetus , Hemolysis , Isoantibodies , Reticulocytes , Retrospective Studies , Sensitivity and SpecificityABSTRACT
Objective To compare the effect of two experiments-Thermal elution test in 56 ℃ and Freezing thawing test in -20℃ for ABO haemolytic disease in neonates.Methods 74 samples of neonatal blood with anticoagulant EDTA-K2 were collected.Di-ana card method was used to compare the difference of the two experiments.Results 66 samples were positive and 8 were negative in 56 ℃.Meanwhile 52 were positive and 22 were negative in -20 ℃.There was significant difference between the two methods (P<0.05).The positive coincidence rate in 56 ℃ was obviously higher than that in -20 ℃.Conclusion There is difference in detec-tion effect between Thermal elution test in 56 ℃ and Freezing thawing test in -20 ℃ for neonatal ABO haemolytic disease.
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We report a case of Ael in a 44-year old woman. The patient s red cells were typed as 0 and her serum had both anti-A and anti-B, but the agglutination strength with Al cell was weaker (2+) than with B cell (4+) in her serum. Additional tests showed that the red cells were not agglutinated by anti-A,B and A antigen on patient s RBC was demonstrated by adsorption-elution test. Her saliva contained H but no A substance, and the ABO genotyping test identified her blood type as AO. We concluded that this was a case of blood type Ael with anti-A. (Korean J Blood Transfusion 10(1): 69 75, 1999)
Subject(s)
Adult , Female , Humans , Agglutination , Blood Transfusion , SalivaABSTRACT
We report a case of Ael in a 44-year old woman. The patient' red cells were typed as O and her serum had both anti-A and anti-B, but the agglutination strength with A1 cell was weaker (2+) than with B cell (4+) in her serum. Additional tests showed that the red cells were not agglutinated by anti-A,B and A antigen on patient' RBC was demonstrated by adsorption-elution test. Her saliva contained H but no A substance, and the ABO genotyping test identified her blood type as AO. We concluded that this was a case of blood type Ael with anti-A.
Subject(s)
Adult , Female , Humans , Agglutination , SalivaABSTRACT
The absorption-elution test using low ionic strength solution (LISS) has been compared with the test using normal saline in MN typing of 258 bloodstain samples stored 1 to 6 years. The accuracy rate was 94.57％ using LISS method. The present study indicated that the LISS method is more sensitive than tests carried out in normal saline.
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This paper reports the elution test of adhering villus on a slide to detect the (?)uman chorion ABO(H)substance of the blood. A preliminary study of sample taking conditions and sample preservation was made for the experiment. The results show that the elution test needs less villi(2-4mg)and is easier to conduct, as compared with other methods. The method is of practical value in identifying the disputed (?)aternity in the early pregnancy. When kept properly in refrigeration, the smaple of chorion cart be kept as long as 3 months.