ABSTRACT
Objective:To study the effect of methylmercury chloride(MMC) on the apoptosis and the expression of apoptosis-associated proteins in the intestinal epithelium of fetal mice.Methods:The duodenum and colon were taken from E13.5d and E14.5d fetal mice respectively,and cultured with different doses of MMC(0 ?g/L,200?g/L,400?g/L,800?g/L) for 24h.The samples were sliced into the paraffin sections.Some sections were stained with HE to count the numbers of apoptotic bodies(NAB) in the fetal intestinal epithelium with stereological method.The others were stained with the immunohistochemical method to observe the expression of apoptosis-associated proteins Bcl-2,Bax,P53 and c-Fos.Results:①In experimental groups,all the NAB in the intestinal epithelium were higher than that in control groups(P
ABSTRACT
Objective To study the effects and related mechanism of low-dosed methyl-mercury chloride (MMC) on the epithelial apoptosis of fetal mice intestine. Methods Pregnant mice on E12.5 d and E13.5 d were injected with different doses of MMC (0, 1, 2, 4 mg/kg) intraperitoneally. After 48 h, their duodenum and colon were dissected out and sliced into the paraffin sections. Some sections were stained with HE to count the numbers of apoptotic bodies (NAB) with stereological method. The others were stained with the immunohistochemical method to observe the expression of apoptosis-associated proteins Bcl-2, Bax and immediate early gene c-fos. Results ①In all experimental groups, the NAB in the epithelium were higher than that of control groups (P
ABSTRACT
Objective:To study the effect of hydrocortisone (HC) on apoptosis and expression of apoptosis- associated proteins in the intestinal epithelium of fetal mice.Methods:The duodena and colons were taken from E13.5d and E14.5d fetal mice respectively,and cultured with different doses of HC (0 ?g/ml,0.5?g/ml,1.0?g/ml,2.0?g/ml).After 24h,the samples were sliced up into the paraffin sections.Some sections were stained with HE to count the numbers of apoptotic bodies (NAB)in the fetal intestinal epithelium with stereological method.Others were stained with the immunohistochemical method to observe the expression of Bcl-2,Bax,P53 and c-Fos.Results:①In each experimental group,the NAB were higher than that of control group( P
ABSTRACT
Objective To study the spatiotemporal distributions of epithelial proliferation and apoptosis,and the correlation of morphogenesis with them in developing stomach.Methods Serial sections of fetal mice aged 11-15.5 days were made.The morphogenesis of stomach was observed under light microscope.The density of mitotic cells(DMC) and apoptotic bodies(DAB) of epithelium in the developing cardia,pylorus,greater curvature,lesser curvature were measured with stereological method.Results The morphological development of stomach took place on the 11~(th) day of mouse embryo development.The fundus,greater curvature and lesser curvature were observed on the 12~(th) day.The peak value of DMC all appeared before the peak value of DAB.In the epithelium of cardia,no obvious peak value of DMC was observed and the peak value of DAB was on the 11.5~(th),12.5~(th),13.5~(th) day.In the epithelium of pylorus,the peak value of DMC was on the 12.5~(th) day,the peak value of DAB was on the 14~(th) day.In the epithelium of greater curvature,the peak value of DMC was on the 11.5~(th) and 12~(th) day,the DAB value was low.In the epithelium of lesser curvature,the peak value of DMC was on the 11.5~(th) day,the peak value of DAB was on the 12.5~(th) day.Conclusion There was very close spatiotemporal relationship between the cell proliferation,cell apoptosis and the morphological development of stomach.