Distribution and Association of Lip Print Pattern, Finger Print Pattern and ABO Blood Groups in Relation to Gender and Family Among South Indian Population

Background: Positive identification of living/ deceased using distinct traits is a cornerstone of forensic science. According to Locard’s principle “When two objects come into contact, there is always transfer of material from one to another”. The finger prints, lip prints and blood remains are the evidence for forensic identification. Aim: To study the frequency, distribution and association of ABO blood groups, finger print pattern, lip print pattern among gender and also the inherent nature of patterns among family members. Materials and Methods: A total of 200 Subjects (100 males, 100 females) within the age range of 17- 30 years and 20 families were randomly selected and aggregated as groups. Lip prints and finger prints were collected using dusting technique with fine carbon powder. ABO blood group was determined by slide agglutination method. Results: Ulnar loop - Males - Vertical, Radial loop - Males - Branching, Whorl - Females - Branching, Arch - Males - Intersecting; Females - Vertical. O positive, B positive - Loop - Branching, A positive - Loop - Vertical and Intersecting, AB positive - Whorl - Branching and Intersecting. Among 20 families, 14 families showed 100 percent pattern resemblance. High percentage of similar pattern was observed between father to daughter (70%) in finger prints and mother to son in lip prints (71%). Conclusion: Correlating the uniqueness of these physical evidence helps in personal identification as it can narrow the search & to substantiate the facts where there is little evidence

Effects of “Green Removing ”Processing Technology of the Fresh Fruit of Schisandra chinensis after Harvested on the Quality of Medicinal Material / 中国药房

OBJECTIVE：To stud y the effects of “green removing ”processing technology of fresh fruit of Schisandra chinensis after harvested on the quality of medicinal material ，and to provide new ideas for the scientific and rational processing of Chinese medicinal material. METHODS ：Fifteen fresh fruits of S. chinensis were used as samples ，with 3 samples in each group. The sample were processed preliminarily by 5 methods，such as drying at 50 ℃，drying in the sun ，drying at 50 ℃ after“green removing”processing with microwave ，drying at 50 ℃ after“green removing ”processing with blanching ，drying at 50 ℃ after “green removing ”processing with steaming. HPLC fingerprints of 15 batches of dried S. chinensis products were established and similarity evaluation was conducted according to Similarity Evaluation System of TCM Chromatographic Fingerprints （2012 edition）. Cluster analysis was used to evaluate the similarity of dried S. chinensis products with different processing methods. At the same time ，HPLC method was adopted to determine the content changes of seven lignans in dried products ，such as schisandrol A ， schisandrol B ，schisantherin A ，schisantherin B ，schizandrin A ，schisandrin B and schisandrin C. RESULTS ：A total of 7 common peaks were obtained in the fingerprints of 15 batches of dried S. chinensis products. Except that the similarity between the chromatograms of dried samples in the sun and the control fingerprint was relatively low ，the similarities of chromatograms of dried products by other processing methods were greater than 0.900. Cluster analysis showed that 6 samples dried at 50 ℃ after“green removing”processing with microwave and dried at 50 ℃ after“green removing ”processing with blanching were grouped into the first category ；3 samples dried at 50 ℃ after“green removing”processing with steaming were grouped into the second category ；6 samples dried at 50 ℃ and dried in sun were grouped into the third category. The content determination results showed that there was no significant difference in the total content of seven lignans in the samples dried at 50 ℃ and dried in the sun （P＞0.05）. The total contents of seven lignans in the samples dried at 50 ℃ after“green moving ” processing with microwave ，blanching and steaming were significantly higher than those dried at 50 ℃ and dried in sun （P＜0.01）. CONCLUSIONS：The quality of S. chinensis samples dried after “green moving ”processing with microwave and blanching is better than those directly dried in sun and dried in oven.

Study of Fingerprint Patterns among Young Adults and its Relation with Their Gender & Blood Groups

Fingerprint patterns are genotypically determined and remain unchanged from birth till death so they are used as effective means of establishing identity of an individual & study of finger prints as method of identification is known as Dactylography or Dactyloscopy. Aim- was to study fingerprint patterns among young adults & its relation with their gender & blood groups. Methods: Study includes 100 young adults of both genders selected randomly.Fingerprints were taken by pressing fingertips of subjects on stamp pad & then transferring the impressions on paper. Subjects were instructed to mention their Blood group, Age & sex on the same paper. Results: Subjects were having 69%,25%,6% of Loops, Whorls & Arches respectively.Males had 68%,26%,6% & females had 69%,24%,7% of Loops,Whorls & Arches respectively.Arches were absent in Rh -ve blood group & were maximum in blood group B+ i-e 50.8% followed by blood groups O+ve,AB+ve & A+ve with 34.4%, 13.1% & 1.7% respectively.Whorls were absent in B-ve Subjects & were maximum in B+vesubjects with 34.8% followed by O+ve, A+ve,AB+ve,O-ve,AB-ve with 28%,22.4%,10.8%, 2.4%,1.6% respectively.Loops were maximum in B+ve i-e 36.6% followed by A+ve,O+ve,AB+ve,O-ve,B-ve, AB-ve with 26.6%,24.5%,6.5%,3.5%, 1.4%,0.9% respectively. Conclusion: Loops are the most common & arches are the least common fingerprint pattern found in the population & Rh- blood groups lack Arches. whorls were absent in B-ve blood group & loops were minimum in AB-ve blood group.Whorls, loops & Arches all types were maximum in B+ve blood group.

Comparison of Bacteria ERIC-PCR Fingerprints of Index Fingers and Contactants / 法医学杂志

Objective To explore the bacteria relevance between index fingers and contactant' surfaces (mobile phone touch screen and desktop of personal office table). Methods Bacteria were collected from the index fingers, mobile phone touch screen and desktop of personal office table of 10 volunteers. Enterobacterial repetitive intergenic consensus(ERIC)-PCR fingerprint was established by PCR amplifi-cation technique of metagenome. Results There were 7 volunteers' ERIC-PCR fingerprints of index fin-gers matched that took from the mobile phone touch screens, and different from each other. There were 3 volunteers' ERIC-PCR fingerprints of index fingers matched that took from desk top of personal office table, and other 7 volunteers' ERIC-PCR fingerprints did not match perfectly with that took from desk top of personal office table,but had at least one similar band for both. Conclusion The bacteria on index finger shows individual specificity, which on mobile phone touching screen and personal desktop may be a new biological sample of forensic identification.

An Efficient Human Identification through MultiModal Biometric System

ABSTRACT Human identification is essential for proper functioning of society. Human identification through multimodal biometrics is becoming an emerging trend, and one of the reasons is to improve recognition accuracy. Unimodal biometric systems are affected by various problemssuch as noisy sensor data,non-universality, lack of individuality, lack of invariant representation and susceptibility to circumvention.A unimodal system has limited accuracy. Hence, Multimodal biometric systems by combining more than one biometric feature in different levels are proposed in order to enhance the performance of the system. A supervisor module combines the different opinions or decisions delivered by each subsystem and then make a final decision. In this paper, a multimodal biometrics authentication is proposed by combining face, iris and finger features. Biometric features are extracted by Local Derivative Ternary Pattern (LDTP) in Contourlet domain and an extensive evaluation of LDTP is done using Support Vector Machine and Nearest Neighborhood Classifier. The experimental evaluations are performed on a public dataset demonstrating the accuracy of the proposed system compared with the existing systems. It is observed that, the combination of face, fingerprint and iris gives better performance in terms of accuracy, False Acceptance Rate, False Rejection Rate with minimum computation time.

Pharmacognostic and HPTLC Fingerprint Profile of the Root of Aristolochia indica Linn. and Quantification of the Marker Compound.

Introduction: Root of Aristolochia indica Linn. has long been used as an oxytoxic agent to aid women in child birth and as abortifacient in Indian folk medicine. It is also one of the ingredients in some traditional Ayurveda medicinal preparations. Aims: The present work has been designed to delineate the pharmacognostic profile of the root of Aristolochia indica Linn and the High-performance thin-layer chromatographic (HPTLC) identification of the active compound and its quantitative estimation in the herbal sample. Materials and Methods: Macroscopic, microscopic evaluation, powder analysis, fluorescence standards of the root of Aristolochia indica Linn and its HPTLC fingerprint profile. Results: Pharmacognostic profile of the root investigated revealed the transverse section possessing somewhat circular outline with tissue organization as outer thin walled cork layers, narrow cortex, and inner cortical cells with groups of stone cells. Secondary xylem tissues were fissured to form narrow strips, wide medullary rays with greater quantities of parenchyma, ray cells with rich deposition of starch. Vessels were solitary and occluded with tyloses and starch grains with 'Maltese cross' were the characteristic features of the taxon. HPTLC method was developed for the estimation of the marker constituent, Aristolochic Acid I (AAI) in dried root sample. Chloroform: Methanol (6:2v/v), was used as mobile phase to separate the analyte. The Rf value for Aristolochic Acid I (C17H11NO7) was found to be 0.53. Calibration plot was established showing the dependence of response on the amount chromatographed. Linearity was found to be in the concentration range of 100 to 500ng/spot for AAI with the correlation coefficient value r=0.998. The result showed that the content of marker compound (AAI) in dried root of Aristochia india Linn was 0.082%. Conclusions: The results of the present study suggest that, the documented morphological descriptors, delineated anatomical markers and developed HPTLC methods are complementary characteristics, which could be effectively used for the identification and authentication of the root of Aristolochia indica Linn.

Anti-inflammatory activity and qualitative analysis of different extracts of Maytenus obscura (A. Rich.) Cuf. by high performance thin layer chromatography method

<p><b>OBJECTIVE</b>To perform aqueous ethanol soluble fraction (AESF) and dichloromethane extract of aerial parts of Maytenus obscura (A. Rich.) Cuf. using high performance thin layer chromatography (HPTLC) and to test anti-inflammatory activity of these extracts.</p><p><b>METHODS</b>HPTLC studies were carried out using CAMAG HPTLC system equipped with Linomat IV applicator, TLC scanner 3, Reprostar 3, CAMAG ADC 2 and WIN CATS-4 software were used. The anti-inflammatory activity was tested by injecting different groups of rats (6 each) with formalin in hind paw and measuring the edema volume before and 1 h later formalin injection. Control group received saline i.p. The extracts treatment was injected i.p. in doses of 100 and 200 mg/kg 1 h before formalin administration. Indomethacin (30 mg/kg) was used as standard.</p><p><b>RESULTS</b>The results of preliminary phytochemical studies confirmed the presence of protein, lipid, carbohydrate, phenol, flavonoid, saponin, triterpenoid, alkaloid and anthraquinone in both extracts. Chromatography was performed on glass-backed silica gel 60 F254 HPTLC plates with the green solvents toluene: ethyacetate: glacial acetic acid (5:3:0.2, v/v/v) as mobile phase. HPTLC finger printing of AESF revealed major eight peaks with Rf values in the range of 0.28 to 0.80 and the dichloromethane revealed major 11 peaks with Rf values in the range of 0.12 to 0.76. The purity of sample was confirmed by comparing the absorption spectra at start, middle and end position of the band. Treatment of rats (i.p.) with AESF and dichloromethane in doses of 100 and 200 mg/kg inhibited singnificantly (P<0.05, n=6) formalin-induced inflammation by 50%, 55.9%, 45.5%, and 51.4%, respectively.</p><p><b>CONCLUSIONS</b>HPTLC finger printing of AESF and dichloromethane of Maytenus obscura revealed eight major spots for alcoholic extracts and nine major spots for dichloromethane extracts. These HPTLC profiles may be of great usefulness in the quality control of herbal products containing these extracts. The anti-inflammatory activity of both extracts also revealed the medicinal importance of these extracts. The plant can be further explored for the isolation of phytoconstituents having anti-inflammatory activity.</p>

Chromatographic finger print analysis of anti-inflammatory active extract fractions of aerial parts of Tribulus terrestris by HPTLC technique

<p><b>OBJECTIVE</b>To develop HPTLC fingerprint profile of anti-inflammatory active extract fractions of Tribulus terrestris (family Zygophyllaceae).</p><p><b>METHODS</b>The anti-inflammatory activity was tested for the methanol and its fractions (chloroform, ethyl acetate, n-butanol and aqueous) and chloroform extract of Tribulus terrestris (aerial parts) by injecting different groups of rats (6 each) with carrageenan in hind paw and measuring the edema volume before and 1, 2 and 3 h after carrageenan injection. Control group received saline i.p. The extracts treatment was injected i.p. in doses of 200 mg/kg 1 h before carrageenan administration. Indomethacin (30 mg/kg) was used as standard. HPTLC studies were carried out using CAMAG HPTLC system equipped with Linomat IV applicator, TLC scanner 3, Reprostar 3, CAMAG ADC 2 and WIN CATS-4 software for the active fractions of chloroform fraction of methanol extract.</p><p><b>RESULTS</b>The methanol extract showed good antiedematous effect with percentage of inhibition more than 72%, indicating its ability to inhibit the inflammatory mediators. The methanol extract was re-dissolved in 100 mL of distilled water and fractionated with chloroform, ethyl acetate and n-butanol. The four fractions (chloroform, ethyl acetate, n-butanol and aqueous) were subjected to anti-inflammatory activity. Chloroform fraction showed good anti-inflammatory activity at dose of 200 mg/kg. Chloroform fraction was then subjected to normal phase silica gel column chromatography and eluted with petroleum ether-chloroform, chloroform-ethyl acetate mixtures of increasing polarity which produced 15 fractions (F1-F15). Only fractions F1, F2, F4, F5, F7, F9, F11 and F14 were found to be active, hence these were analyzed with HPTLC to develop their finger print profile. These fractions showed different spots with different Rf values.</p><p><b>CONCLUSIONS</b>The different chloroform fractions F1, F2, F4, F5, F7, F9, F11 and F14 revealed 4, 7, 7, 8, 9, 7, 7 and 6 major spots, respectively. The results obtained in this experiment strongly support and validate the traditional uses of this Sudanese medicinal plant.</p>

Chromatographic finger print analysis of anti-inflammatory active extract fractions of aerial parts of Tribulus terrestris by HPTLC technique

Objective:To develop HPTLC fingerprint profile of anti-inflammatory active extract fractions of Tribulus terrestris (family Zygophyllaceae). Methods:The anti-inflammatory activity was tested for the methanol and its fractions (chloroform, ethyl acetate, n-butanol and aqueous) and chloroform extract of Tribulus terrestris (aerial parts) by injecting different groups of rats (6 each) with carrageenan in hind paw and measuring the edema volume before and 1, 2 and 3 h after carrageenan injection. Control group received saline i.p. The extracts treatment was injected i.p. in doses of 200 mg/kg 1 h before carrageenan administration. Indomethacin (30 mg/kg) was used as standard. HPTLC studies were carried out using CAMAG HPTLC system equipped with Linomat IV applicator, TLC scanner 3, Reprostar 3, CAMAG ADC 2 and WIN CATS-4 software for the active fractions of chloroform fraction of methanol extract. Results:The methanol extract showed good antiedematous effect with percentage of inhibition more than 72%, indicating its ability to inhibit the inflammatory mediators. The methanol extract was re-dissolved in 100 mL of distilled water and fractionated with chloroform, ethyl acetate and n-butanol. The four fractions (chloroform, ethyl acetate, n-butanol and aqueous) were subjected to anti-inflammatory activity. Chloroform fraction showed good anti-inflammatory activity at dose of 200 mg/kg. Chloroform fraction was then subjected to normal phase silica gel column chromatography and eluted with petroleum ether-chloroform, chloroform-ethyl acetate mixtures of increasing polarity which produced 15 fractions (F1-F15). Only fractions F1, F2, F4, F5, F7, F9, F11 and F14 were found to be active, hence these were analyzed with HPTLC to develop their finger print profile. These fractions showed different spots with different Rf values. Conclusions:The different chloroform fractions F1, F2, F4, F5, F7, F9, F11 and F14 revealed 4, 7, 7, 8, 9, 7, 7 and 6 major spots, respectively. The results obtained in this experiment strongly support and validate the traditional uses of this Sudanese medicinal plant.

Anti-inflammatory activity and qualitative analysis of different extracts of Maytenus obscura (A. Rich.) Cuf. by high performance thin layer chromatography method

Objective: To perform aqueous ethanol soluble fraction (AESF) and dichloromethane extract of aerial parts of Maytenus obscura (A. Rich.) Cuf. using high performance thin layer chromatography (HPTLC) and to test anti-inflammatory activity of these extracts.Methods:HPTLC studies were carried out using CAMAG HPTLC system equipped with Linomat IV applicator, TLC scanner 3, Reprostar 3, CAMAG ADC 2 and WIN CATS-4 software were used. The anti-inflammatory activity was tested by injecting different groups of rats (6 each) with formalin in hind paw and measuring the edema volume before and 1 h later formalin injection. Control group received saline i.p. The extracts treatment was injected i.p. in doses of 100 and 200 mg/kg 1 h before formalin administration. Indomethacin (30 mg/kg) was used as standard.Results:The results of preliminary phytochemical studies confirmed the presence of protein, lipid, carbohydrate, phenol, flavonoid, saponin, triterpenoid, alkaloid and anthraquinone in both extracts. Chromatography was performed on glass-backed silica gel 60 F254 HPTLC plates with the green solvents toluene: ethyacetate: glacial acetic acid (5:3:0.2, v/v/v) as mobile phase. HPTLC finger printing of AESF revealed major eight peaks with Rf values in the range of 0.28 to 0.80 and the dichloromethane revealed major 11 peaks with Rf values in the range of 0.12 to 0.76. The purity of sample was confirmed by comparing the absorption spectra at start, middle and end position of the band. Treatment of rats (i.p.) with AESF and dichloromethane in doses of 100 and 200 mg/kg inhibited singnificantly (P<0.05, n=6) formalin-induced inflammation by 50%, 55.9%, 45.5%, and 51.4%, respectively.Conclusions:HPTLC finger printing of AESF and dichloromethane of Maytenus obscura revealed eight major spots for alcoholic extracts and nine major spots for dichloromethane extracts. These HPTLC profiles may be of great usefulness in the quality control of herbal products containing these extracts. The anti-inflammatory activity of both extracts also revealed the medicinal importance of these extracts. The plant can be further explored for the isolation of phytoconstituents having anti-inflammatory activity.

Finger print pattern in different blood groups.

Dermatoglyphics is study of pattern of fine ridges on fingers, palms and soles. The term dermatoglyphics was coined by Cummins. The type of finger print is unique and is based on genetic characters of each individual. They develop between 2nd and 3rd months of intra uterine life and remain unchanged in an individual through out life. Finger prints are regarded as the most reliable tool for personal identification. Due to their immence potential particularly in forensic medicine, the study of finger print pattern was carried out in relation to various ABO blood groups. The study was carried out in the Department of Anatomy, NRI medical college, Chinakakani, Guntur. 506 students of known blood group were selected for the study. The finger prints were collected, studied and analyzed statistically. Thumbs presented high frequency of whorls in A+ves. Index and ring fingers were associated with high frequency of whorls in A-ves and AB+ves.

A primary study of the different protein expression profiles between human oral squamous cell carcinoma and normal oral mucosa tissues / 实用口腔医学杂志

Objective: To identify the different protein expression profiles between human oral squamous cell carcinoma (OSCC) and normal oral mucosa tissues, and provide experimental data for further study of the development mechanism of OSCC. Methods: 10 cases of OSCC and paired normal oral mucosa tissues were collected and analyzed through two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Results: (1) The average protein spots of OSCC were 2 325±390, while that of normal oral mucosa tissues were 2 487±281. (2) 29 differential protein spots were found between OSCC and normal oral mucosa. Moreover, these protein spots were all down- regulated in OSCC compared with normal oral mucosa. Among these spots, 3 were identified as fibrin beta, triosephosphate isomerase (TIM) and unknown protein through mass spectrometry and bioinformation. Conclusion: Down-regulation of fibrin beta, Triosephosphate isomerase(TIM) and unknown protein are found in the development of OSCC and the mechanism needs further study.

Pattern of finger-prints in different ABO blood groups.

Finger print (dactylography / dermatoglyphic) is considered as the best tool of identification. This study was carried out in 2000-2001 on 300 students of different ABA blood groups of Medical College, Ajmer with two objectives, viz. (a) To study distribution of finger print pattern among the subjects having different ABO and Rh blood group and (b) Correlate any relation between their characters and blood groups. Male: female ratio was 2.4:1. Majority of the subjects (38.33%) in the study were of blood group A followed by blood group B, A and AB of whom 95.67% were Rh-positive. The general distribution of pattern of finger print showed high frequency (51.87%) of loops whereas whorls were moderate (35.83%) and arches were least (12.30%) in frequency. Almost same order was noticed in both Rh-positive and Rhnegative individuals or A, B, AB and O blood groups. Blood group A showed more loops (Rh +ve 54.26%, Rh -ve 60%) while, blood group AB had more whorls (Rh +ve 43.34%, Rh -ve60%). The study suggests an association between finger print pattern and blood group. The distribution of different pattern of finger prints in individual fingers also showed some peculiarities in relation to blood group. The total finger ridge count (TFRC) was significantly greater in blood group B.

Screening of differential expression proteins from human oral lichen planus and normal oral mucosa by two-dimensional difference gel electrophoresis and mass spectrometry / 实用口腔医学杂志

Objective:To find biomarkers for oral lichen planus by comparing differential expressing proteins. Methods:10 cases of oral lichen planus and normal oral mucosa tissues were collected.Total protein was extracted; differential proteome profiles were established and analyzed by two-dimensional polyacrylamide gel electrophoresis(2D-PAGE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Results:(1)The well-resolved,reproducible 2-DE patterns of oral lichen planus and normal oral mucosa were obtained. The results showed that average protein spots were 1 576?67 and 1 608?73 in oral lichen planus and normal oral mucosa respectively, (2) The 13 differential protein spots were identified by Imaging Master 2D image analysis software between oral lichen planus and normal oral mucosa. There were 7 protein spots in oral lichen planus were higher than those in normal oral mucosa, 6 protein spots in oral lichen planus were lower than those in normal oral mucosa. 10 differential expressing proteins were analyzed by mass spectrometry and bioinformation. 4 of them were well characterized including manganese superoxide dismutase (Mn-SOD), Annexin I, vimentin and unknown proteins. Conclusion:Differential expression proteins might be candidate biomarkers for diagnosis of oral lichen planus;and proteomic technique is valuable for screening the diagnostic biomarkers.

Morphologic Characteristics of Finger and Palm Prints of the Akha and Lahu in Northern Thailand / 체질인류학회지

In this study of the physical anthropological characteristics of the Akha and Lahu in northern Thailand, whose customs are similar to the Koreans, the authors examined the finger and palm prints of the Akha (male 107, female 114) and Lahu (male 92, female 101) using qualitative methods, and compared them with those of various ethnic groups including Koreans. The results were as follows : The whorl types (Akha 57.7%, Lahu 58.1%) were the most common finger prints, followed by ulnar loop types (Akha 39.7%, Lahu 38.7%), arch types (Akha 2.6%, Lahu 2.8%), and radial loop types (Akha 1.7%, Lahu 1.2%). Of the palm print types, 9-7-5 (Akha 32.7%, Lahu 21.3%) and 7-5-5(Akha 25.4%, Lahu 21.3%) were most common, followed by 11-O-7 (Akha 2.0%, Lahu 11.3%), 9-O-5 (Akha 1.5%, Lahu 10.0%), 11-9-7 (Akha 6.0%, Lahu 5.7%), 7-5-4 (Akha 7.0%, Lahu 2.2%). The finger and palm print patterns of the Akha and Lahu did not correspond closely to the patterns of those classified as White, Negro, or Oriental, although they were closest to the patterns of the latter, particularly Chinese (rather than Korean or Japanese).

High-speed Counter-current Chromatography and Finger Print / 中成药

In this article, the author reported the recent development in study on high-speed counter-current chromatography (HSCCC) and finger print, work principle and technology speciality of HSCCC, and noted that the prospect of HSCCC application in separation and analysis of Chinese medicines and herbal medicines is broad as rapid development of the pharmacentical industry of Chinese medicine, even though the application is still in its infancy at present.

Serum Pharmacochemistry Changes of Guan Xin Ⅱ Oral Decoction in Healthy Volunteers / 中国药房

OBJECTIVE:To study the presence of the chemical compositions of Guan XinⅡ(GXEH)oral decoction in human serum.METHODS:The analysis of finger prints of the medicated serum sample obtained from the volunteers treated with GXEH decoction was conducted with3different pretreatment procedures and2different HPLC methods,and which were compared with decoction.RESULTS:The numbers of the discrepant chromatographic peaks detected from the decoction and the medicated serum were respectively less than30and10,danshensu,protocatechuic aldehyde,ligustrazine,paeoniflorin were failed to be traced in the latter.CONCLUSION:The numbers of chemical compositions of the compound preparation that can be traced in serum were limited and the active components that entered into body may be relatively limited in numbers.After oral administration of compound preparation,a part of chemical compositions of which may not presented as its original form in serum.