ABSTRACT
Senna contains anthraquinones, flavonoids, polysaccharide and volatile oil and other chemical substances, which show the effect of diarrhea, antibacterial, hemostatic, regulating immune function and antioxidation. This article reviewed the Senna chemical composition, pharmacological effects, clinical application and new formulations of drug development, in order to provide reference for the research and clinical application of Senna.
ABSTRACT
Objective To investigate the protective effect of ω?6 soybean oil fat emulsion on folium sennae?induced diarrhea in mice. Methods Thirty?six Kunming mice were randomly divided into three groups,including control,diarrhea,ω?6 soybean oil fat emulsion group(12 mice in each group). Besides the mice in control group,other mice were administrated folium sennae by gavage for 15 days to establish the diarrhea model. Then mice in ω?6 soybean oil fat emulsion group received ω?6 soybean oil fat emulsion by intravenously administration at a dose of 15 mL/kg daily since 6th day after intragastric administration of folium sennae for 10 days. Animals in control group and diarrhea group were intravenously adminis?tered with same volume of saline. The body weight ,general state and diarrhea index of the mice in each group were dynamically assessed. Ten days after intravenous injection,mice in every group were sacrificed and tissues were collected. Morphology of intestine mucosa was observed after HE staining. Albumin(ALB)level in plasma was evaluated by biochemical method. Proliferating cell nuclear antigen(PCNA)expression in intestine mucosa were assessed by immunohistochemical method. Results Compared with that in the diarrhea group,the general status,body weight and diarrhea index of mice in ω?6 soybean oil fat emulsion group were improved significantly. Ten days after intravenously administration ,pathological change in intestine mucosa of mice in ω?6 soybean oil fat emulsion group was improved significantly ,ALB level in plasma and PCNA expression in intestine mucosa were significantly increased(P<0.05)compared with that in diarrhea group. Conclusion ω?6 soybean oil fat emulsion has a significant protective effect on the diarrhea of mice induced by folium sennae ,which may be related to the up?regulated expression of PCNA by ω?6 soybean oil fat emulsion.
ABSTRACT
[Objective] To observe the effect of long-term gavage of Folium Sennae (FS) on colic electromyogram and interstitial cells of Cajal (ICC) in rats. [Methods] Forty rats were randomized into normal control group and FS group. The normal control group was fed with conventional forage. FS group was fed with dry forage mixed with FS powder for 3 months, the preliminary dose of FS was 200mg?kg-1?d-1 and then increased progressively by 100mg?kg-1?d-1 to ensure over half of the rats having diarrhea, and the final dose was 2800 mg?kg-1?d-1. The frequency and amplitude of colic slow wave were detected and the changes of myenteric plexus and ICC were observed by zinc iodide -osmic acid staining method. [Results] After long-term gavage of FS, the frequency and amplitude of colic slow wave in rats obviously decreased ( P
ABSTRACT
AIM:To establish the quality standard for Tongbianling Capsule(Folium Sennae,Radix Angelicae sinensis,Herba Cistanches). METHODS: Folium Sennae,Radix Angelicae sinensis of Tongbianling Capsuls were identified by TLC.Cisttanoside and echinacoside were determined by HPLC together.The HPLC system consisted of shim-pack c_(18) column(4.6 mm?150 mm,5 ?m),acotonitrile-methanol-0.2% pure phosphoric water solution(7∶10∶83) mixture as mobile phase,flow rate of 1.0 mL/min,detection wavelength at 334 nm. RESULTS: The TLC method was suitable for the identification of salidroside and ptyrosol with good reproducibility.The quantitative evaluation of cistanoside had the linear range of 0.114 9-2.298 ?g,the average recovery was 98.2%(RSD=1.70%,n=6).The quantitative evaluation of echinacoside had the linear range of 0.147 9-2.958 ?g,the average recovery was 96.8%(RSD=1.53%,n=6). CONCLUSION: The TLC and HPLC method is found to have good stability and reproducibility for Tongbianling Capsule.